{"id":2812,"date":"2024-05-30T21:43:40","date_gmt":"2024-05-30T13:43:40","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2812"},"modified":"2024-09-03T17:12:02","modified_gmt":"2024-09-03T09:12:02","slug":"5-reasons-to-automate","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/5-reasons-to-automate\/","title":{"rendered":"\u81ea\u52d5\u5316 NGS \u5de5\u4f5c\u6d41\u7a0b\u7684 5 \u500b\u7406\u7531"},"content":{"rendered":"\n

\u8207\u7b2c\u4e00\u4ee3\u65b9\u6cd5\u76f8\u6bd4\uff0c\u65b0\u4e00\u4ee3\u5b9a\u5e8f (NGS) \u5177\u6709\u66f4\u5feb\u7684\u901f\u5ea6\u3001\u66f4\u4f4e\u7684\u6210\u672c\u548c\u66f4\u9ad8\u7684\u901a\u91cf\uff0c\u5df2\u5c07\u751f\u7269\u5b78\u7814\u7a76\u63a8\u5165\u4e86\u4e00\u500b\u65b0\u6642\u4ee3\u3002 \u5168\u57fa\u56e0\u7d44\u5b9a\u5e8f\u3001\u8868\u89c0\u57fa\u56e0\u7d44\u5b78\u3001\u5b8f\u57fa\u56e0\u7d44\u5b78\u548c\u5fae\u751f\u7269\u7d44\u5b78\u53ea\u662f\u7576\u4eca\u773e\u591a NGS \u61c9\u7528\u4e2d\u7684\u4e00\u5c0f\u90e8\u5206\uff0c\u5b83\u5011\u4fc3\u9032\u4e86\u6d41\u884c\u75c5\u5b78\u3001\u764c\u75c7\u8a3a\u65b7\u548c\u85e5\u7269\u9818\u57df\u7684\u9032\u6b65\u3002<\/p>\n\n\n\n

\"\u81ea\u52a8\u5316<\/figure>\n\n\n\n

\u8207\u7b2c\u4e00\u4ee3\u65b9\u6cd5\u76f8\u6bd4\uff0c\u6b21\u4e16\u4ee3\u5b9a\u5e8f (NGS) \u5177\u6709\u66f4\u5feb\u7684\u901f\u5ea6\u3001\u66f4\u4f4e\u7684\u6210\u672c\u548c\u66f4\u9ad8\u7684\u901a\u91cf\uff0c\u5df2\u5c07\u751f\u7269\u5b78\u7814\u7a76\u63a8\u5165\u4e86\u4e00\u500b\u65b0\u6642\u4ee3\u3002 \u5168\u57fa\u56e0\u7d44\u5b9a\u5e8f\u3001\u8868\u89c0\u57fa\u56e0\u7d44\u5b78\u3001\u5b8f\u57fa\u56e0\u7d44\u5b78\u548c\u5fae\u751f\u7269\u7d44\u5b78\u53ea\u662f\u7576\u4eca\u773e\u591aNGS \u61c9\u7528\u7684\u4e00\u5c0f\u90e8\u5206\uff0c\u5b83\u5011\u4fc3\u9032\u4e86\u6d41\u884c\u75c5\u5b78\u3001\u764c\u75c7\u8a3a\u65b7\u548c\u85e5\u7269\u767c\u73fe\u7684\u9032\u6b65\uff0c\u4e26\u6975\u5927\u5730\u63d0\u9ad8\u4e86\u6211\u5011\u5c0d\u751f\u7269\u9ad4\u5982\u4f55\u5229\u7528\u907a\u50b3\u8a0a\u606f\u5728\u74b0\u5883\u4e2d\u751f\u5b58\u548c\u7e41\u6b96\u7684\u7406\u89e3\u3002 \u4e0d\u65b7\u8b8a\u5316\u7684\u74b0\u5883\u3002<\/p>\n\n\n\n

\u5e02\u5834\u4e0a\u6709\u5404\u7a2e\u5404\u6a23\u7684\u6e2c\u5e8f\u5100\u5668\u548c\u670d\u52d9\u63d0\u4f9b\u5546\uff0cNGS \u6b63\u8fc5\u901f\u88ab\u5404\u7a2e\u898f\u6a21\u548c\u4e0d\u540c\u9810\u7b97\u7684\u5be6\u9a57\u5ba4\u6240\u63a5\u53d7\u3002 \u96d6\u7136\u5b9a\u5e8f\u548c\u6578\u64da\u5206\u6790\u901a\u5e38\u662f\u81ea\u52d5\u5316\u7684\uff0c\u4f46\u4e0a\u6e38 NGS \u6b65\u9a5f\uff0c\u5373\u6a23\u54c1\u8655\u7406\u548c\u6587\u5eab\u88fd\u5099\u901a\u5e38\u662f\u624b\u52d5\u9032\u884c\u7684\u3002 \u5e78\u904b\u7684\u662f\uff0c\u900f\u904e\u4e00\u4e9b\u52aa\u529b\u548c\u512a\u5316\uff0c\u9019\u4e9b\u6b65\u9a5f\u53ef\u4ee5\u5728\u79fb\u6db2\u6a5f\u5668\u4eba\u4e0a\u8f15\u9b06\u81ea\u52d5\u5316\uff0c\u70ba\u4f7f\u7528\u8005\u7bc0\u7701\u4e86\u8a31\u591a\u6f5b\u5728\u7684\u9ebb\u7169\u3002 \u5728\u9019\u88e1\uff0c\u6211\u5011\u4f86\u770b\u770b\u81ea\u52d5\u5316 NGS \u5de5\u4f5c\u6d41\u7a0b\u7684 5 \u5927\u7406\u7531\u3002<\/p>\n\n\n\n

    \n
  1. \u53ef\u64f4\u5c55\u6027<\/strong><\/li>\n<\/ol>\n\n\n\n

    \u5982\u679c\u60a8\u66fe\u7d93\u5617\u8a66\u904e\u624b\u52d5\u8655\u7406\u5927\u91cf NGS \u7d50\u5408\u6a23\u672c\uff0c\u90a3\u9ebc\u64f4\u5927\u898f\u6a21\u7684\u6f5b\u529b\u61c9\u8a72\u6703\u8b93\u60a8\u5c0d\u81ea\u52d5\u5316\u611f\u5230\u8208\u596e\u3002 \u6a5f\u5668\u4eba\u7684\u5177\u9ad4\u529f\u80fd\u53d6\u6c7a\u65bc\u578b\u865f\uff0c\u4f46\u5728\u5927\u591a\u6578\u60c5\u6cc1\u4e0b\uff0c\u60a8\u5c07\u80fd\u5920\u540c\u6642\u8655\u7406\u591a\u9054 96 \u500b\u6a23\u672c\uff0c\u5f9e\u800c\u986f\u8457\u6e1b\u5c11\u624b\u52d5\u8655\u7406\u76f8\u540c\u6578\u91cf\u7684\u6a23\u672c\u6240\u9700\u7684\u6642\u9593\u3002<\/p>\n\n\n\n

    \u81ea\u52d5\u5316\u9084\u70ba\u60a8\u7701\u53bb\u4e86\u6279\u91cf\u8655\u7406\u6a23\u54c1\u7684\u53ef\u6015\u9ebb\u7169\uff0c\u7531\u65bc\u96e2\u5fc3\u6a5f\u5c3a\u5bf8\u3001\u6a23\u54c1\u964d\u89e3\u98a8\u96aa\uff08\u4f8b\u5982\uff0c\u4f7f\u7528 RNA \u6642\uff09\u4ee5\u53ca\u6700\u5927\u6578\u91cf\u7684\u9650\u5236\uff0c\u9019\u5728\u624b\u52d5\u8a2d\u5b9a\u4e2d\u901a\u5e38\u662f\u5fc5\u8981\u7684\u3002 \u4eba\u5011\u5728\u4efb\u4f55\u6642\u5019\u90fd\u611f\u89ba\u53ef\u4ee5\u8f15\u9b06\u8655\u7406\u7684\u6a23\u672c\u3002 \u6bd4\u907f\u514d\u9ebb\u7169\u66f4\u91cd\u8981\u7684\u662f\uff0c\u66f4\u5c11\u7684\u6279\u6b21\u610f\u5473\u8457\u66f4\u5c11\u6216\u6c92\u6709\u6279\u6b21\u9593\u7684\u5dee\u7570\u3002<\/p>\n\n\n\n

    2\u3001\u5275\u65b0<\/strong><\/p>\n\n\n\n

    \u900f\u904e\u81ea\u52d5\u5316\u64f4\u5927 NGS \u5be6\u9a57\u898f\u6a21\u5c07\u4e0d\u53ef\u907f\u514d\u5730\u8b93\u60a8\u80fd\u5920\u89e3\u6c7a\u624b\u52d5\u8a2d\u5b9a\u5e7e\u4e4e\u7121\u6cd5\u89e3\u6c7a\u7684\u65b0\u7814\u7a76\u554f\u984c\u3002 \u4ee5\u4e0b\u662f\u4e00\u7cfb\u5217 NGS \u61c9\u7528\u7684\u5e7e\u500b\u7bc4\u4f8b\uff1a<\/p>\n\n\n\n

    \u85e5\u7269\u767c\u73fe\uff1a\u7be9\u9078\u5927\u91cf\u65b0\u578b\u5316\u5408\u7269\uff0c\u4ee5\u5c0b\u627e\u75be\u75c5\u6750\u6599\uff08\u4f8b\u5982\u7d30\u80de\u7cfb\uff09\u4e2d\u6240\u9700\u7684\u8f49\u9304\u6216\u8868\u89c0\u907a\u50b3\u5b78\u8b8a\u5316\uff0c\u800c\u4e0d\u662f\u8a66\u5716\u6311\u9078\u53ef\u80fd\u5177\u6709\u6cbb\u7642\u76f8\u95dc\u4f5c\u7528\u7684\u5316\u5408\u7269\u3002<\/p>\n\n\n\n

    \u5fae\u751f\u7269\u7d44\u5b78\uff1a\u5206\u6790\u8db3\u5920\u5927\u7684\u6a23\u672c\u96c6\uff0c\u4ee5\u7372\u5f97\u60a8\u9078\u64c7\u7684\u7269\u7a2e\u4e2d\u300c\u6b63\u5e38\u300d\u5fae\u751f\u7269\u5340\u7cfb\u7684\u5716\u7247\u3002 \u5982\u679c\u60a8\u4e00\u6b21\u53ea\u80fd\u8655\u7406 12 \u6216 24 \u500b\u6a23\u672c\uff0c\u9019\u5c07\u662f\u4e00\u9805\u4e0d\u53ef\u80fd\u7684\u4efb\u52d9\u3002<\/p>\n\n\n\n

    \u690d\u7269\u80b2\u7a2e\uff1a\u5927\u898f\u6a21\u9451\u5b9a\u8fb2\u85dd\u91cd\u8981\u57fa\u56e0\u548c\u907a\u50b3\u6a19\u8a18\uff0c\u4ee5\u5e6b\u52a9\u57fa\u56e0\u7d44\u9078\u64c7\u548c\u57fa\u56e0\u5de5\u7a0b\u958b\u767c\u5177\u6709\u9ad8\u5ea6\u7406\u60f3\u6027\u72c0\u7684\u65b0\u54c1\u7a2e\u3002<\/p>\n\n\n\n

    \u75be\u75c5\u7814\u7a76\u8207\u8a3a\u65b7\uff1a\u7be9\u9078\u5927\u91cf\u81e8\u5e8a\u6a23\u672c\u4ee5\u767c\u73fe\u65b0\u7684\u75be\u75c5\u76f8\u95dc\u57fa\u56e0\u6216\u751f\u7269\u6a19\u8a18\u3002<\/p>\n\n\n\n

    \u74b0\u5883\u76e3\u6e2c\uff1a\u900f\u904e\u5c0d\u5176\u7121\u810a\u690e\u52d5\u7269\u5c45\u6c11\u9032\u884c\u5b9a\u6027\u548c\u5b9a\u91cf\u5e8f\u5217\u5206\u6790\u4f86\u8a55\u4f30\u751f\u614b\u7cfb\u7d71\u7684\u5065\u5eb7\u72c0\u6cc1\u3002<\/p>\n\n\n\n

    \u9664\u4e86\u589e\u52a0\u60a8\u81ea\u5df1\u7684\u7814\u7a76\u5275\u65b0\u4e4b\u5916\uff0c\u66f4\u9ad8\u7684\u901a\u91cf\u9084\u61c9\u8a72\u4fc3\u9032\u8207\u5176\u4ed6\u5be6\u9a57\u5ba4\u52a0\u5f37\u5408\u4f5c\uff0c\u70ba\u65b0\u5c08\u6848\u548c\u767c\u73fe\u6253\u958b\u5927\u9580\uff0c\u5426\u5247\u9019\u4e9b\u5c08\u6848\u548c\u767c\u73fe\u5c07\u975e\u5e38\u5177\u6709\u6311\u6230\u6027\uff08\u5982\u679c\u53ef\u80fd\u7684\u8a71\uff09\u3002<\/p>\n\n\n\n

      \n
    1. \u4e00\u81f4\u7684\u6578\u64da\u8cea\u91cf<\/li>\n<\/ol>\n\n\n\n

      \u5728\u6574\u500bNGS\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff0c\u6a23\u672c\u8655\u7406\u3001\u6587\u5eab\u5efa\u7f6e\u4ee5\u53caPCR\u64f4\u589e\u548c\u6587\u5eab\u6a19\u6e96\u5316\u7b49\u4e0b\u6e38\u8655\u7406\u6b65\u9a5f\u7279\u5225\u5bb9\u6613\u51fa\u73fe\u4eba\u70ba\u932f\u8aa4\u3002 \u8a72\u5354\u8b70\u5f88\u5197\u9577\uff0c\u6709\u8a31\u591a\u79fb\u6db2\u6b65\u9a5f\u548c\u4e0d\u65b7\u8b8a\u5316\u7684\u8a66\u5291\u9ad4\u7a4d\uff0c\u901a\u5e38\u9700\u8981\u6cbf\u9014\u5c07\u6a23\u54c1\u8f49\u79fb\u5230\u65b0\u7ba1\u4e2d\u3002 \u4e0d\u6b63\u78ba\u7684\u6a19\u7c64\u3001\u6c61\u67d3\u3001\u79fb\u6db2\u932f\u8aa4\u6216\u8207\u65b9\u6848\u7684\u6700\u8f15\u5fae\u504f\u5dee\u90fd\u6703\u56b4\u91cd\u5f71\u97ff\u7d50\u679c\u6578\u64da\u3002<\/p>\n\n\n\n

      \u5373\u4f7f\u4eba\u70ba\u932f\u8aa4\u5b8c\u5168\u4e0d\u53ef\u907f\u514d\uff0c\u6279\u6b21\u4e4b\u9593\u7684\u5dee\u7570\u4ee5\u53ca\u5de5\u4f5c\u4eba\u54e1\u4e4b\u9593\u6a23\u54c1\u8655\u7406\u7684\u5dee\u7570\u4e5f\u662f\u4e0d\u53ef\u907f\u514d\u7684\u3002 \u5982\u679c\u53ef\u80fd\u7684\u8a71\uff0c\u76e3\u63a7 NGS \u5de5\u4f5c\u6d41\u7a0b\u4e2d\u7684\u9019\u4e9b\u932f\u8aa4\u548c\u8b8a\u66f4\u4e5f\u662f\u4e0d\u5207\u5be6\u969b\u7684\u3002 \u81ea\u52d5\u5316\u6a23\u54c1\u548c\u6587\u5eab\u88fd\u5099\u7de9\u89e3\u4e86\u9019\u500b\u554f\u984c\uff0c\u63d0\u4f9b\u66f4\u597d\u7684\u904e\u7a0b\u63a7\u5236\u3001\u6e96\u78ba\u9ad8\u6548\u7684\u5de5\u4f5c\u6d41\u7a0b\u4ee5\u53ca\u7368\u7acb\u65bc\u4eba\u54e1\u8b8a\u52d5\u7684\u66f4\u597d\u7684\u904b\u884c\u91cd\u8907\u6027\uff0c\u5f9e\u800c\u7121\u8ad6\u60a8\u67091 \u500b\u9084\u662f1,000 \u500b\u6a23\u54c1\uff0c\u90fd\u53ef\u4ee5\u63d0\u4f9b\u53ef\u9760\u6027\u548c\u4e00\u81f4\u7684\u6578\u64da\u54c1\u8cea\u3002<\/p>\n\n\n\n

        \n
      1. \u589e\u52a0\u96e2\u958b\u6642\u9593<\/strong><\/li>\n<\/ol>\n\n\n\n

        \u9019\u4e26\u4e0d\u5b8c\u5168\u662f\u9ed1\u767d\u5206\u660e\u7684\uff0c\u56e0\u70ba\u60a8\u9700\u8981\u5728\u555f\u52d5\u968e\u6bb5\u6295\u5165\u4e00\u4e9b\u6642\u9593\u548c\u7cbe\u529b\uff0c\u4ee5\u78ba\u4fdd\u60a8\u7684\u79fb\u6db2\u6a5f\u5668\u4eba\u80fd\u5920\u57f7\u884c\u56b4\u683c\u7684\u5354\u8b70\u3002\u5982\u679c\u5e78\u904b\u7684\u8a71\uff0c\u60a8\u4e5f\u8a31\u80fd\u5920\u627e\u5230\u4e00\u500b \u5f9e\u5176\u4f9b\u61c9\u5546\u6216\u60a8\u9078\u64c7\u7684\u6838\u9178\u63d0\u53d6\u548c\u6587\u5eab\u88fd\u5099\u8a66\u5291\u76d2\u7684\u4f9b\u61c9\u5546\u8655\u7372\u53d6\u9069\u5408\u60a8\u6a5f\u5668\u4eba\u7684\u8173\u672c\uff0c\u5176\u4e2d\u8a31\u591a\u4f9b\u61c9\u5546\u8207\u81ea\u52d5\u5316\u4f9b\u61c9\u5546\u5408\u4f5c\u958b\u767c\u81ea\u52d5\u5316\u5c31\u7dd2\u5354\u8b70\u3002<\/p>\n\n\n\n

        \u7121\u8ad6\u5982\u4f55\uff0c\u4e00\u65e6\u60a8\u7684\u81ea\u52d5\u5316\u8a2d\u5b9a\u555f\u52d5\u4e26\u904b\u884c\uff0c\u60a8\u5c31\u53ef\u4ee5\u4eab\u53d7\u5c07\u5bf6\u8cb4\u7684\u5be6\u9a57\u5ba4\u6642\u9593\u82b1\u5728\u898f\u5283\u65b0\u7684\u95dc\u9375\u5be6\u9a57\u4e0a\uff0c\u800c\u4e0d\u662f\u91cd\u8907\u7684\u6a23\u54c1\u548c\u6587\u5eab\u6e96\u5099\u3002 \u81ea\u52d5\u5316\u9084\u53ef\u4ee5\u6e1b\u5c11\u5c0d\u54e1\u5de5\u9032\u884c\u591a\u7a2e\u4e0d\u540c\u65b9\u6848\u57f9\u8a13\u6240\u9700\u7684\u5de5\u4f5c\u91cf\uff0c\u5f9e\u800c\u91cb\u653e\u66f4\u591a\u8cc7\u6e90\uff0c\u4e26\u907f\u514d\u54e1\u5de5\u56e0\u904e\u5ea6\u79fb\u6db2\u800c\u9020\u6210\u7684\u91cd\u8907\u6027\u52de\u640d\u3002<\/p>\n\n\n\n

          \n
        1. \u964d\u4f4e\u6210\u672c<\/strong><\/li>\n<\/ol>\n\n\n\n

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