{"id":2826,"date":"2024-05-30T22:40:43","date_gmt":"2024-05-30T14:40:43","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2826"},"modified":"2024-09-03T14:12:52","modified_gmt":"2024-09-03T06:12:52","slug":"opentrons-covid-19","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/opentrons-covid-19\/","title":{"rendered":"\u4f7f\u7528 Opentrons \u9032\u884c COVID-19 \u6aa2\u6e2c"},"content":{"rendered":"\n

Opentrons \u7684 COVID-19 \u6aa2\u6e2c\u7cfb\u7d71\u53ef\u4ee5\u5e6b\u52a9\u904b\u884c\u516c\u5171\u885b\u751f\u76e3\u6e2c\u8a08\u756b\u7684\u5be6\u9a57\u5ba4\u7acb\u5373\u64f4\u5927\u898f\u6a21\uff0c\u6bcf\u5929\u7be9\u6aa2 2,400 \u6b21\u6aa2\u6e2c\u3002 Opentrons \u7684\u4f7f\u547d\u662f\u900f\u904e\u66f4\u5ee3\u6cdb\u5730\u4f7f\u7528\u5be6\u9a57\u5ba4\u6a5f\u5668\u4eba\u4f86\u52a0\u901f\u751f\u547d\u79d1\u5b78\u7684\u767c\u5c55\u3002\u4e16\u754c\u73fe\u5728\u6bd4\u4ee5\u5f80\u4efb\u4f55\u6642\u5019\u90fd\u66f4\u9700\u8981\u9019\u7a2e\u80fd\u529b\u3002\u5927\u898f\u6a21\u7684 COVID-19 \u6aa2\u6e2c\u662f\u9650\u5236\u6b7b\u4ea1\u7387\u7684\u6700\u4f73\u65b9\u6cd5\u4e4b\u4e00\u3002\u5927\u898f\u6a21\u6aa2\u6e2c\u53ef\u4ee5\u5feb\u901f\u767c\u73fe\u548c\u9694\u96e2\u611f\u67d3\uff0c\u9650\u5236\u75c5\u6bd2\u7684\u50b3\u64ad\u4e26\u4fdd\u8b77\u8106\u5f31\u65cf\u7fa4\u3002\u70ba\u4e86\u6b63\u78ba\u8abf\u67e5\u4eba\u7fa4\uff0c\u9700\u8981\u8655\u7406\u6578\u767e\u842c\u500b COVID-19 \u6aa2\u6e2c\u8a66\u5291\u76d2\u3002\u4e16\u754c\u5404\u5730\u7684\u7d44\u7e54\u90fd\u5728\u52aa\u529b\u76e1\u5feb\u63d0\u9ad8\u5176\u80fd\u529b\u3002<\/p>\n\n\n\n

\u9019\u5c31\u662f Opentrons \u70ba\u61c9\u5c0d\u9019\u7a2e\u6311\u6230\u800c\u5efa\u7acb\u7684\u3002<\/p>\n\n\n\n

\"COVID-19<\/figure>\n\n\n\n

Opentrons \u7684 COVID-19 \u6aa2\u6e2c\u7cfb\u7d71\u662f\u904b\u884c COVID-19 \u516c\u5171\u885b\u751f\u76e3\u6e2c\u8a08\u756b\u7684\u5be6\u9a57\u5ba4\u7684\u6700\u4f73\u89e3\u6c7a\u65b9\u6848\uff0c\u9019\u4e9b\u5be6\u9a57\u5ba4\u9700\u8981\u7acb\u5373\u64f4\u5c55\u5230\u81ea\u52d5\u5316\u64cd\u4f5c\u3002 Opentrons \u53ef\u4ee5\u5728\u4e0b\u8a02\u55ae\u5f8c\u7684\u5e7e\u5929\u5167\u5b89\u88dd\u6bcf\u5929\u81ea\u52d5\u9032\u884c\u591a\u9054 2,400 \u6b21\u6e2c\u8a66\u7684\u7cfb\u7d71\u3002\u6211\u5011\u76ee\u524d\u6b63\u5728\u52a0\u5dde\u5e15\u6d1b\u963f\u723e\u8a17\u7684\u958b\u653e\u91ab\u5b78\u7814\u7a76\u6240\u90e8\u7f72\u9019\u7a2e\u76e3\u6e2c\u80fd\u529b\uff0c\u5f88\u5feb\u5c31\u6703\u6709\u66f4\u591a\u76e3\u6e2c\u8a08\u756b\u3002<\/p>\n\n\n\n

\u6211\u5011\u53ef\u4ee5\u8207\u4efb\u4f55\u8a66\u5291\u4f9b\u61c9\u5546\u5408\u4f5c\uff0c\u751a\u81f3\u63d0\u4f9b\u6700\u4f73\u548c\u6700\u5be6\u60e0\u7684\u9078\u64c7\u5efa\u8b70\u3002\u5982\u679c\u60a8\u7684\u5be6\u9a57\u5ba4\u9700\u8981\u64f4\u5927 COVID-19 \u76e3\u6e2c\u696d\u52d9\uff0c\u8acb\u900f\u904e covid-19@opentrons.com \u8207\u6211\u5011\u806f\u7d61\u3002<\/p>\n\n\n\n

Opentrons \u548c\u958b\u653e\u91ab\u5b78\u7814\u7a76\u6240\u6e34\u671b\u8207\u7576\u5730\u548c\u806f\u90a6\u6a5f\u69cb\u5408\u4f5c\uff0c\u78ba\u5b9a\u8a72\u7cfb\u7d71\u662f\u5426\u53ef\u5728\u7dca\u6025\u4f7f\u7528\u6388\u6b0a (EUA) \u4e0b\u7528\u65bc\u81e8\u5e8a\u8a3a\u65b7\uff0c\u4e26\u63d0\u4f9b\u6e2c\u8a66\u983b\u5bec\u4ee5\u6eff\u8db3\u65e5\u76ca\u589e\u9577\u7684\u6975\u9ad8\u9700\u6c42\u3002<\/p>\n\n\n\n

\u4ee5\u4e0b\u662f\u6709\u95dc\u6211\u5011\u958b\u767c\u7684\u7cfb\u7d71\u4ee5\u53ca\u5982\u4f55\u5c07\u5176\u90e8\u7f72\u7528\u65bc\u5927\u898f\u6a21 COVID-19 \u6e2c\u8a66\u7684\u8a73\u7d30\u8cc7\u8a0a\u3002<\/p>\n\n\n\n

\u6e2c\u8a66\u904e\u7a0b\u8207\u8a31\u591a\u50b3\u67d3\u75c5\u6aa2\u6e2c\u4e00\u6a23\uff0c\u6aa2\u6e2c COVID-19 \u75c5\u6bd2\u7684\u6aa2\u6e2c\u63a1\u7528\u5b9a\u91cf\u805a\u5408\u9176\u9023\u9396\u53cd\u61c9 (qPCR) \u6280\u8853\u3002\u5c0d\u65bc\u50cf COVID-19 \u9019\u6a23\u7684\u51a0\u72c0\u75c5\u6bd2\uff0c\u8a72\u6aa2\u6e2c\u5be6\u969b\u4e0a\u662f\u4e00\u7a2e\u9006\u8f49\u9304\u9176 qPCR (RT-qPCR)\uff0c\u56e0\u70ba\u5b83\u6e2c\u91cf\u7684\u662f RNA \u800c\u4e0d\u662f DNA\u3002 RT-qPCR \u80fd\u5920\u6aa2\u6e2c\u5f9e\u60a3\u8005\u6a23\u672c\u4e2d\u7d14\u5316\u7684 RNA \u4e2d\u76ee\u6a19 COVID-19 \u5e8f\u5217\u7684\u5b58\u5728\u548c\u6c34\u5e73\uff0c\u5f9e\u800c\u544a\u8a34\u60a8\u67d0\u4eba\u662f\u5426\u88ab\u611f\u67d3\uff0c\u5982\u679c\u88ab\u611f\u67d3\uff0c\u5927\u7d04\u611f\u67d3\u4e86\u591a\u9577\u6642\u9593\u3002<\/p>\n\n\n\n

\u7b2c\u4e00\u6b65\u662f\u6536\u96c6\u6a23\u672c\u3002\u6536\u96c6\u767c\u751f\u5728\u91ab\u751f\u4e0b\u4ee4\u9032\u884c\u6e2c\u8a66\u5f8c\u3002\u91ab\u9662\u3001\u5be6\u9a57\u5ba4\u548c\u516c\u5171\u885b\u751f\u6a5f\u69cb\u64e6\u62ed\u60a3\u8005\u7684\u9f3b\u5b50\u6216\u5634\u5df4\uff0c\u4e26\u7acb\u5373\u5c07\u6e2c\u8a66\u62ed\u5b50\u653e\u5165\u8a66\u7ba1\u4e2d\u3002\u7136\u5f8c\uff0c\u9019\u4e9b\u60a3\u8005\u6a23\u672c\u88ab\u5b89\u5168\u5730\u5e36\u5230\u6aa2\u6e2c\u5be6\u9a57\u5ba4\u3002<\/p>\n\n\n\n

\u4e00\u65e6\u6536\u96c6\u7ba1\u5230\u9054\u5be6\u9a57\u5ba4\uff0c\u6709\u4e09\u500b\u81ea\u52d5\u5316\u6b65\u9a5f\uff1a<\/p>\n\n\n\n

1.\u6a23\u672c\u63a5\u7a2e2.RNA \u8403\u53d63.qPCR \u8a2d\u7f6e<\/p>\n\n\n\n

\u6bcf\u500b\u6b65\u9a5f\u90fd\u6709\u6307\u5b9a\u7684 OT-2 \u5de5\u4f5c\u7ad9\uff08\u6aa2\u6e2c\u9664\u5916\uff0c\u6aa2\u6e2c\u5728\u5be6\u9a57\u5ba4\u63d0\u4f9b\u7684 qPCR \u5100\u5668\u4e0a\u9032\u884c\uff09\u3002\u4ee5\u4e0b\u662f\u5982\u4f55\u5728 Opentrons \u4e0a\u5be6\u73fe\u6d41\u7a0b\u81ea\u52d5\u5316\uff1a<\/p>\n\n\n\n

\u7ad9\u9ede A \u2013 \u6a23\u672c\u92ea\u677f\uff1a\u5c07 96 \u500b\u88dd\u6709\u61f8\u6d6e\u5728\u5931\u6d3b\u7de9\u885d\u6db2\u4e2d\u7684\u60a3\u8005\u6a23\u672c\u7684\u6536\u96c6\u7ba1\u653e\u5728\u7b2c\u4e00\u500b Opentrons \u6a5f\u5668\u4eba\u4e0a\uff0c\u91cd\u65b0\u683c\u5f0f\u5316\u4ee5\u63d0\u53d6 RNA\u3002\u6a5f\u5668\u4eba\u5c07\u6a23\u672c\u548c\u967d\u6027\u5c0d\u7167\u5f9e\u7ba1\u4e2d\u79fb\u6db2\u5230\u6df1\u5b54\u677f\u4e2d\u3002<\/p>\n\n\n\n

\u7ad9\u9ede A \u914d\u7f6e\uff1a9,050 \u7f8e\u5143<\/p>\n\n\n\n

p20 + p300 \u55ae\u901a\u9053\u79fb\u6db2\u7ba1\u6eab\u5ea6\u6a21\u7d44\uff08\u4e3b\u52d5\u51b7\u537b\u5c0d\u7167RNA\uff094x \u7ba1\u67b696 \u6df1\u5b54\u8403\u53d6\u677f1x 20ul \u67b6+ 1x 200ul \u6ffe\u82af\u5438\u982d\u67b6\u7ad9\u9edeA \u7684OT-2 \u7532\u677f\u4f48\u5c40\u7ad9\u9edeA \u7684OT-2 \u7532\u677f\u4f48\u5c40\u7ad9\u9edeA \u61c9\u8a72\u4f4d\u65bc\u67d0\u7a2e\u300c\u9032\u6a23\u300d\u5ba4\u3002\u6b64\u7db2\u7ad9\u7684\u4e3b\u8981\u74f6\u9838\u901a\u5e38\u4e0d\u662f\u5be6\u969b\u7684\u91cd\u65b0\u683c\u5f0f\u5316\uff0c\u800c\u662f\u70ba\u6a5f\u5668\u4eba\u8cbc\u4e0a\u6a19\u7c64\u3001\u8a18\u9304\u3001\u958b\u84cb\u548c\u5c07\u6536\u96c6\u7ba1\u88dd\u5165\u67b6\u5b50\u3002\u6700\u597d\u5c07\u6a23\u54c1\u9032\u6a23\u5ba4\u8207\u9032\u884c\u6a23\u54c1\u8655\u7406\u7684\u4efb\u4f55\u7a7a\u9593\u5206\u958b\uff0c\u4ee5\u9632\u6b62\u6c61\u67d3\u3002\u4e00\u65e6\u6a23\u672c\u548c\u967d\u6027\u5c0d\u7167 RNA \u653e\u5165\u6df1\u5b54\u677f\uff0c\u5373\u53ef\u79fb\u81f3 B \u7ad9\u3002<\/p>\n\n\n\n

B \u7ad9 \u2013 RNA \u8403\u53d6\uff1a96 \u500b\u6df1\u5b54\u677f\uff08\u5305\u542b\u6a23\u672c\uff09\u79fb\u81f3\u7b2c\u4e8c\u500b\u6a5f\u5668\u4eba\uff0c\u4f7f\u7528\u78c1\u6027\u5fae\u73e0\u9032\u884c RNA \u8403\u53d6\u3002\u9019\u662f\u81ea\u52d5\u5316\u904e\u7a0b\u7684\u4e3b\u8981\u90e8\u5206\uff0c\u56e0\u70ba\u9700\u8981\u591a\u6b21\u6e05\u6d17\u6b65\u9a5f\u624d\u80fd\u5b8c\u5168\u7d14\u5316 RNA\u3002<\/p>\n\n\n\n

B \u7ad9\u914d\u7f6e\uff1a7,750 \u7f8e\u5143<\/p>\n\n\n\n

p300 8 \u901a\u9053\u79fb\u6db2\u5668\u78c1\u6027\u6a21\u7d4496 \u6df1\u5b54\u8403\u53d6\u677f96 \u5b54\u6d17\u812b\u677f5 \u500b 200ul \u6ffe\u5634\u67b62 \u500b 12 \u901a\u9053\u8a66\u5291\u5bb9\u56682 \u500b 1 \u901a\u9053\u6db2\u9ad4\u5ee2\u68c4\u7269\u5bb9\u5668<\/p>\n\n\n\n

\u9084\u61c9\u8a72\u6709\u4e00\u500b\u5c08\u9580\u7684\u63d0\u53d6\u5ba4\uff0c\u7528\u65bc\u5b89\u7f6e\u6240\u6709 B \u7ad9\u63d0\u53d6\u6a5f\u5668\u4eba\u3002\u5982\u679c\u541e\u5410\u91cf\u9700\u8981\uff0c\u9019\u500b\u623f\u9593\u9084\u53ef\u4ee5\u5bb9\u7d0d\u4e00\u500b\u8a66\u5291\u586b\u5145\u7ad9\uff0c\u70ba B \u7ad9\u6a5f\u5668\u4eba\u6e96\u5099\u78c1\u6027\u5fae\u73e0\u548c\u7de9\u885d\u6db2\uff0c\u4ee5\u9032\u884c RNA \u8403\u53d6\u3002\u5982\u60a8\u5728\u4e0a\u9762\u7684\u7532\u677f\u5716\u4e2d\u6240\u898b\uff0c\u6b64\u904e\u7a0b\u9700\u8981\u5927\u91cf\u8a66\u5291\u548c\u5438\u982d\u3002<\/p>\n\n\n\n

C \u7ad9 - \u6aa2\u6e2c\u8a2d\u5b9a\uff1a\u5f9e\u6a23\u672c\u4e2d\u5206\u96e2\u51fa\u7684 RNA\uff08\u6d17\u812b\uff09\u96a8\u5f8c\u88ab\u79fb\u81f3\u7b2c\u4e09\u500b\u623f\u9593\uff0c\u8a72\u623f\u9593\u5bb9\u7d0d qPCR \u5100\u5668\uff0c\u4e26\u8a2d\u8a08\u70ba\u76e1\u53ef\u80fd\u201c\u4e7e\u6de8\u201d\uff0c\u4ee5\u6700\u5927\u9650\u5ea6\u5730\u6e1b\u5c11\u6c61\u67d3\u3002\u5c07\u88dd\u6709\u7d14\u6a23\u672c RNA \u7684\u677f\u5b50\u653e\u5728 Opentrons \u6a5f\u5668\u4eba\u4e0a\uff0c\u70ba RT-qPCR \u6aa2\u6e2c\u505a\u6e96\u5099\u3002<\/p>\n\n\n\n

C \u7ad9 OT-2 \u914d\u7f6e\uff1a7,500 \u7f8e\u5143<\/p>\n\n\n\n

p20 \u55ae\u901a\u9053Opentrons \u6eab\u5ea6\u6a21\u7d4496 \u5b54\u6d17\u812b\u677f96 \u5b54 PCR \u677f3x \u67b6 20ul \u904e\u6ffe\u5438\u982d<\/p>\n\n\n\n

RT-qPCR \u6240\u9700\u7684\u8a66\u5291\u5206\u5225\u653e\u7f6e\u5728\u6eab\u5ea6\u6a21\u7d44\u4e0a\u9032\u884c\u4e3b\u52d5\u51b7\u537b\u3002\u9019\u4e9b\u8a66\u5291\u88ab\u7d44\u5408\u6210\u6bcd\u6db2\u6df7\u5408\u7269\uff0c\u7136\u5f8c\u88ab\u5206\u914d\u5230\u677f\u4e2d\u4e26\u8207\u7d14\u5316\u7684 RNA \u7d50\u5408\u3002\u7d93\u904e\u6b64\u904e\u7a0b\u5f8c\uff0c\u677f\u5c31\u53ef\u4ee5\u653e\u5165 qPCR \u6a5f\u5668\u4e2d\u4e26\u5206\u6790\u662f\u5426\u6709 COVID-19\u3002<\/p>\n\n\n\n

\u541e\u5410\u91cf\u6211\u5011\u958b\u767c\u4e86\u4e00\u500b\u7531 8 \u500b\u6a5f\u5668\u4eba\u7d44\u6210\u7684\u7cfb\u7d71\uff082 \u500b A \u7ad9\u30014 \u500b B \u7ad9\u30012 \u500b C \u7ad9\uff09\uff0c\u53ef\u4ee5\u6bcf 12 \u5c0f\u6642\u81ea\u52d5\u6e96\u5099 12 \u500b 96 \u5b54\u677f\uff0c\u6216\u6bcf\u5929\u5927\u7d04 2,400 \u6b21\u6e2c\u8a66\u3002<\/p>\n\n\n\n

8 \u500b\u6a5f\u5668\u4eba\u7cfb\u7d71\u7684\u6d41\u7a0b\u6642\u9593\u5b89\u6392\u5982\u4e0b\uff1a<\/p>\n\n\n\n

\u8a72\u7cfb\u7d71\u52a0\u4e0a\u5099\u4ef6\u548c\u5be6\u9a57\u5ba4\u5b89\u88dd\u8cbb\u7528\u70ba 100,000 \u7f8e\u5143\uff0c\u53ef\u7528\u65bc\u76e3\u6e2c\u9805\u76ee\u3002\u6211\u5011\u53ef\u4ee5\u5728 3 \u6708\u6bcf\u9031\u5b89\u88dd 1 \u6b21\uff0c\u7136\u5f8c\u5728 4 \u6708\u6bcf\u9031\u5b89\u88dd 4 \u6b21\u3002\u5982\u679c\u6211\u5011\u5be6\u73fe\u9019\u4e00\u76ee\u6a19\uff0c\u6211\u5011\u9810\u8a08\u5728 6 \u6708\u5e95\u4e4b\u524d\u5728 Opentrons \u7cfb\u7d71\u4e0a\u81ea\u52d5\u5b8c\u6210 100 \u842c\u6b21\u6e2c\u8a66\u3002<\/p>\n\n\n\n

\u6211\u5011\u76ee\u524d\u6b63\u5728\u52aa\u529b\u5728\u52a0\u5dde\u5e15\u6d1b\u963f\u723e\u8a17\u7684\u958b\u653e\u91ab\u5b78\u7814\u7a76\u6240\u5b89\u88dd\u7b2c\u4e00\u500b\u9ad8\u901a\u91cf\u7cfb\u7d71\uff0c\u4e26\u6e34\u671b\u4f7f\u7528\u6211\u5011\u7684\u6a5f\u5668\u4eba\u4f86\u5e6b\u52a9\u5be6\u9a57\u5ba4\u5b8c\u6210\u9019\u500b\u95dc\u9375\u9805\u76ee\u3002<\/p>\n\n\n\n

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