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\"\u9762\u5411\u751f\u547d\u79d1\u5b66\u7814\u7a76\u4eba\u5458\u7684<\/figure>\n\n\n\n

\u5168\u7403 Slack \u983b\u9053<\/p>\n\n\n\n

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\u5f48\u51fa\u5f0f\u5075\u6e2c\u5be6\u9a57\u5ba4\u85cd\u5716<\/p>\n\n\n\n

\u5275\u65b0\u57fa\u56e0\u7d44\u5b78\u7814\u7a76\u6240 (IGI) \u63d0\u4f9b\u4e86\u6709\u95dc\u5982\u4f55\u5efa\u7acb CLIA \u8a8d\u8b49\u7684 SARS-CoV-2 \u6aa2\u6e2c\u5be6\u9a57\u5ba4\u7684\u8a73\u76e1\u6307\u5357\u3002 IGI \u662f\u52a0\u5dde\u5927\u5b78\u67cf\u514b\u840a\u5206\u6821\u548c\u52a0\u5dde\u5927\u5b78\u820a\u91d1\u5c71\u5206\u6821\u7684\u7814\u7a76\u4eba\u54e1\u5718\u968a\uff0c\u4ed6\u5011\u7d44\u5efa\u4e86\u4e00\u652f\u591a\u5143\u5316\u3001\u8de8\u5b78\u79d1\u7684\u5718\u968a\uff0c\u665d\u591c\u4e0d\u505c\u5730\u5de5\u4f5c\u4e86 3 \u9031\uff0c\u4ee5\u64f4\u5927\u7063\u5340\u7684\u51a0\u72c0\u75c5\u6bd2\u6aa2\u6e2c\u898f\u6a21\u3002\u4ed6\u5011\u7684\u6307\u5357\u5305\u62ec\u5f9e\u7279\u5b9a\u81ea\u52d5\u5316\u5de5\u4f5c\u6d41\u7a0b\u548c\u5354\u8b70\u5230\u4eba\u54e1\u57f9\u8a13\u548c\u5408\u898f\u6307\u5357\u7b49\u5404\u65b9\u9762\u7684\u63d0\u793a\u3002<\/p>\n\n\n\n

\u5b78\u8853\u5408\u4f5c\u8a3a\u65b7\u5be6\u9a57\u5ba4\u7684\u6a19\u6e96\u4f5c\u696d\u7a0b\u5e8f (SOP)<\/p>\n\n\n\n

\u514b\u91cc\u514b COVID19 \u806f\u76df\u63d0\u4f9b\u4e86\u6709\u95dc\u4ed6\u5011\u5982\u4f55\u5728\u5b78\u8853\u74b0\u5883\u4e2d\u8207\u5916\u90e8\u5408\u4f5c\u5925\u4f34\u4e00\u8d77\u5275\u5efa\u8a3a\u65b7\u5be6\u9a57\u5ba4\u7684\u9010\u6b65\u6307\u5357\u3002\u514b\u91cc\u514b COVID19 \u806f\u76df\u8207\u502b\u6566\u5927\u5b78\u5b78\u9662\u91ab\u9662\u3001\u5065\u5eb7\u670d\u52d9\u5be6\u9a57\u5ba4 (HSL)\u3001\u764c\u75c7\u7814\u7a76\u6240\u548c\u5f17\u6717\u897f\u65af\u514b\u91cc\u514b\u7814\u7a76\u6240\u5408\u4f5c\uff0c\u5728\u77ed\u77ed\u5169\u9031\u591a\u7684\u6642\u9593\u5167\u5275\u5efa\u4e86\u9019\u9805\u6e2c\u8a66\u8a2d\u5b9a\u3002\u4ed6\u5011\u7684\u53ef\u4e0b\u8f09\u6307\u5357\u6db5\u84cb\u4e86\u5f9e\u5be6\u9a57\u5ba4\u5b89\u5168\u8cc7\u8a0a\u548c 2 \u985e\u6a23\u672c\u6ec5\u6d3b\u6307\u5357\u5230 RNA \u8403\u53d6\u65b9\u6848\u548c\u88fd\u4f5c\u81ea\u88fd\u7de9\u885d\u6db2\u7684\u6240\u6709\u5167\u5bb9\u3002<\/p>\n\n\n\n

\u81e8\u5e8a\u7814\u7a76\u5b9a\u5e8f\u6e2c\u8a66\u5e73\u53f0<\/p>\n\n\n\n

Broad Institute \u958b\u653e\u4e86\u6709\u95dc\u5176\u91dd\u5c0d SARS-CoV-2 \u7684\u81e8\u5e8a\u7814\u7a76\u5b9a\u5e8f\u5e73\u53f0 (CRSP) \u7684\u5b58\u53d6\u6b0a\u9650\u3002 Broad Institute \u662f\u9ebb\u7701\u7406\u5de5\u5b78\u9662 (MIT) \u548c\u54c8\u4f5b\u5927\u5b78\u7814\u7a76\u4eba\u54e1\u4e4b\u9593\u7684\u5408\u4f5c\uff0c\u5b83\u4f7f\u7528\u4ed6\u5011\u7684\u5e73\u53f0\u904b\u884c\u5373\u6642\u9006\u8f49\u9304\u9176 (RT)-PCR \u8a3a\u65b7\u6aa2\u6e2c\u3002\u5c0d\u65bc\u4efb\u4f55\u5c0b\u627e\u6e2c\u8a66\u8a2d\u65bd\u7684\u7814\u7a76\u4eba\u54e1\uff0cBroad Institute \u90fd\u958b\u653e\u4e86\u4ed6\u5011\u7684\u8a2d\u65bd\u3002\u4ed6\u5011\u7684\u8aaa\u660e\u5305\u62ec\u6a19\u672c\u6536\u96c6\u548c\u904b\u8f38\u6307\u5357\uff0c\u4ee5\u53ca\u91dd\u5c0d\u60a3\u8005\u548c\u63d0\u4f9b\u8005\u7684\u8a3a\u65b7\u6e2c\u8a66\u548c\u6aa2\u6e2c\u60c5\u6cc1\u8aaa\u660e\u66f8\u7684\u5e38\u898b\u554f\u984c\u89e3\u7b54\u3002<\/p>\n\n\n\n

\u57fa\u56e0\u7d44\u5de5\u7a0b\u5de5\u5177<\/p>\n\n\n\n

Synthego \u70ba\u57fa\u56e0\u7d44\u5b78\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u4e86\u4e00\u7cfb\u5217\u5de5\u5177\uff0c\u4ee5\u63d0\u9ad8 COVID-19 \u8a3a\u65b7\u548c\u6cbb\u7642\u7684\u901f\u5ea6\u548c\u6709\u6548\u6027\u3002\u9019\u4e9b\u5de5\u5177\u5305\u62ec\u7528\u65bc RNA \u6536\u96c6\u7684\u5ba2\u88fd\u5316 Cas13 \u6307\u5357\u548c\u91dd\u5c0d\u8207 SARS-CoV-2 \u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684 330 \u500b\u4eba\u985e\u57fa\u56e0\u7684\u591a\u9663\u5217 CRISPR sgRNA \u57fa\u56e0\u6572\u9664\u6587\u5eab\u3002\u4ed6\u5011\u7684\u7db2\u7ad9\u4e0a\u6709\u6240\u6709\u9019\u4e9b\u5de5\u5177\u7684\u93c8\u63a5\uff0c\u4ee5\u53ca\u4ed6\u5011\u7528\u65bc\u5275\u5efa\u9019\u4e9b\u5de5\u5177\u7684\u5df2\u767c\u5e03\u6e2c\u8a66\u6578\u64da\u3002<\/p>\n\n\n\n

SwabSeq \u6e2c\u8a66\u6982\u5ff5\u9a57\u8b49<\/p>\n\n\n\n

Octant \u7684 SwabSeq NGS \u8a3a\u65b7\u5de5\u4f5c\u6d41\u7a0b\u6982\u5ff5\u9a57\u8b49\u3002 Octant \u662f\u4e00\u5bb6\u751f\u7269\u6280\u8853\u516c\u53f8\uff0c\u4f7f\u7528\u5c08\u6709\u6280\u8853\u7d50\u5408\u5408\u6210\u751f\u7269\u5b78\u3001\u57fa\u56e0\u7d44\u5de5\u7a0b\u3001\u4e0b\u4e00\u4ee3\u5b9a\u5e8f\u548c\u8a08\u7b97\u5de5\u5177\uff0c\u5275\u5efa\u4e86\u4e00\u7a2e\u5354\u8b70\u4f86\u64f4\u5927\u57fa\u65bc COVID19 \u5e8f\u5217\u7684\u6e2c\u8a66\uff0c\u8a72\u5354\u8b70\u7c21\u55ae\u3001\u4fbf\u5b9c\u4e14\u9748\u654f\u3002 \u5b83\u88ab\u7a31\u70ba SwabSeq\uff0c\u4ed6\u5011\u7684\u5be6\u9a57\u5ba4\u7b46\u8a18\u6587\u4ef6\u5305\u62ec\u5f9e\u5354\u8b70\u548c\u6e2c\u8a66\u7a0b\u5f0f\u78bc\u5230\u8cc7\u6599\u5716\u8868\u548c\u5167\u90e8\u7b46\u8a18\u672c\u7684\u6240\u6709\u5167\u5bb9\u3002<\/p>\n\n\n\n

\u5927\u898f\u6a21\u5e73\u884c\u8a3a\u65b7\u6e2c\u5b9a\u6982\u5ff5\u9a57\u8b49<\/p>\n\n\n\n

\u8cd3\u5dde\u5dde\u7acb\u5927\u5b78\u5408\u6210\u751f\u7269\u5b78\u7814\u7a76\u54e1 Howard Salis \u7684\u5927\u898f\u6a21\u5e73\u884c NGS \u8a3a\u65b7\u6e2c\u5b9a\u6982\u5ff5\u9a57\u8b49\u3002 Salis \u767c\u8868\u4e86\u4e00\u4efd\u9810\u5370\u672c\uff0c\u8a73\u7d30\u4ecb\u7d39\u4e86\u5927\u898f\u6a21\u5e73\u884c\u8a3a\u65b7\u6e2c\u5b9a(MPDA) \u5982\u4f55\u5229\u7528\u6b21\u4e16\u4ee3\u5b9a\u5e8f\u5728\u4e00\u6b21\u6e2c\u5b9a\u4e2d\u540c\u6642\u9032\u884c\u6578\u5343\u6b21\u75c5\u6bd2RNA \u6e2c\u91cf\uff0c\u4e26\u5728\u6bcf\u500b\u5de5\u4f5c\u6d41\u7a0b\u4e2d\u8655\u7406\u591a\u905419,200 \u500b\u60a3\u8005\u6a23\u672c\u3002\u4ed6\u7684\u9810\u5370\u672c\u8a73\u7d30\u4ecb\u7d39\u4e86\u4ed6\u7684\u5de5\u4f5c\u6d41\u7a0b\uff0c\u7d50\u5408\u4e86\u75c5\u6bd2RNA \u63d0\u53d6\u3001\u4f7f\u7528\u5177\u6709\u7368\u7279\u689d\u78bc\u7684\u60a3\u8005\u7279\u7570\u6027\u5f15\u5b50\u7684\u9006\u8f49\u9304\u9176(RT) \u4ee5\u53ca\u55ae\u6c60\u805a\u5408\u9176\u93c8\u53cd\u61c9(PCR)\uff0c\u7136\u5f8c\u9032\u884c\u4e0b\u4e00\u4ee3\u6e2c\u5e8f(NGS)\u3002<\/p>\n\n\n\n

\u884c\u52d5\u6e2c\u8a66\u5be6\u9a57\u5ba4<\/p>\n\n\n\n

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