{"id":2900,"date":"2024-06-13T20:17:52","date_gmt":"2024-06-13T12:17:52","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=2900"},"modified":"2024-09-03T11:16:43","modified_gmt":"2024-09-03T03:16:43","slug":"rhsyzdhtsrxhyjssj","status":"publish","type":"knowledge2","link":"https:\/\/zh-tw.opentrons.com.cn\/knowledge2\/rhsyzdhtsrxhyjssj\/","title":{"rendered":"\u5982\u4f55\u4f7f\u7528\u81ea\u52d5\u5316\u684c\u4e0a\u578b\u71b1\u5faa\u74b0\u5100\u7bc0\u7701\u5be6\u9a57\u5ba4\u6642\u9593"},"content":{"rendered":"<!-- wp:paragraph -->\n<p><strong>PCR \u6280\u8853\u7684\u7701\u6642\u6f14\u9032<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5728\u6db2\u9ad4\u8655\u7406\u5668\u7684\u5e73\u53f0\u4e0a\u4f7f\u7528\u548c\u4e0d\u5e73\u53f0\u4e0a\u4f7f\u7528\u81ea\u52d5\u71b1\u5faa\u74b0\u5100\u7684\u5340\u5225\u5728\u65bc\u56de\u6536\u7684\u6642\u9593\u3002\u5982\u679c\u5be6\u9a57\u5ba4\u88e1\u6709\u516d\u500b\u984d\u5916\u7684\u5c0f\u6642\uff0c\u4f60\u6703\u600e\u9ebc\u505a\uff1f<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u8207\u751f\u547d\u672c\u8eab\u4e00\u6a23\uff0c\u73fe\u4ee3\u751f\u7269\u6280\u8853\u662f\u57fa\u65bc\u7cbe\u78ba\u8907\u88fd DNA \u7684\u80fd\u529b\u3002\u751f\u7269\u5b78\u5bb6\u52ab\u6301\u4e86\u7d30\u80de\u4e2d\u5167\u5efa\u7684\u6838\u9178\u8907\u88fd\u6a5f\u5236\uff0c\u5275\u5efa\u4e86\u805a\u5408\u9176\u934a\u5f0f\u53cd\u61c9 (PCR)\u2014\u2014\u9019\u662f\u73fe\u4ee3\u5206\u5b50\u751f\u7269\u5b78\u7684\u57fa\u77f3\u65b9\u6cd5\uff0c\u5141\u8a31\u5b83\u5011\u81ea\u5df1\u8907\u88fd DNA\u3002 1983 \u5e74\uff0c\u8afe\u8c9d\u723e\u734e\u5f97\u4e3b\u51f1\u88e1\u00b7\u7a46\u5229\u65af (Kary Mullis) \u8868\u660e\uff0c\u60a8\u53ef\u4ee5\u900f\u904e\u7cbe\u78ba\u7684\u6eab\u5ea6\u5e8f\u5217\u4f86\u5354\u8abf\u6a23\u672c\uff0c\u5f9e\u800c\u64cd\u7e31\u76ee\u6a19 DNA \u5e8f\u5217\u7684\u8907\u88fd\u3002\u7576\u6642\uff0c\u9032\u884c PCR \u5be6\u9a57\u610f\u5473\u8457\u79d1\u5b78\u5bb6\u9700\u8981\u82b1\u8cbb\u6578\u5c0f\u6642\u624b\u52d5\u5c07\u8a66\u7ba1\u79fb\u5165\u548c\u79fb\u51fa\u4e0d\u540c\u6eab\u5ea6\u7684\u6c34\u6d74\uff0c\u4ee5\u5be6\u73fe\u9019\u4e9b\u6eab\u5ea6\u5faa\u74b0\u3002 1988 \u5e74\uff0cCeltis \u548c Perkin-Elmer \u63a8\u51fa\u4e86\u4e00\u7a2e\u540d\u70ba\u71b1\u5faa\u74b0\u5100\u7684\u8a2d\u5099\uff0c\u7528\u65bc\u81ea\u52d5\u57f7\u884c PCR \u5be6\u9a57\u6240\u9700\u7684\u7cbe\u78ba\u6eab\u5ea6\u8b8a\u5316\u3002\u5b83\u6bd4\u6c34\u6d74\u6cd5\u66f4\u5feb\u3001\u66f4\u6709\u6548\u7387\uff0c\u4f46\u6210\u672c\u8d85\u904e 10 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-->\n\n<!-- wp:paragraph -->\n<p>\u5982\u4eca\uff0cPCR \u7684\u7701\u6642\u5275\u65b0\u4f3c\u4e4e\u505c\u6eef\u4e0d\u524d\u3002\u5118\u7ba1\u5df2\u7d93\u5275\u5efa\u4e86\u65b0\u578b PCR\uff08\u5982\u5b9a\u91cf\u548c\u6578\u4f4d PCR\uff09\uff0c\u4f46\u81ea 1990 \u5e74\u4ee3\u4e2d\u671f\u4ee5\u4f86\uff0c\u5728\u7701\u6642\u65b9\u9762\u4e26\u6c92\u6709\u53d6\u5f97\u91cd\u5927\u9032\u5c55\u3002\u5927\u591a\u6578\u751f\u7269\u5b78\u5bb6\u4ecd\u5728\u4f7f\u7528\u8207 20 \u5e74\u524d\u767c\u660e\u7684\u71b1\u5faa\u74b0\u5100\u6280\u8853\u4e0a\u985e\u4f3c\u7684\u71b1\u5faa\u74b0\u5100\uff0c\u4e26\u4e14\u6bcf\u9031\u4ecd\u8981\u82b1\u8cbb\u6578\u5c0f\u6642\u624b\u52d5\u6e96\u5099\u53cd\u61c9\u3002 Opentrons \u71b1\u5faa\u74b0\u5100\u4ee3\u8868\u4e86 PCR \u4f7f\u7528\u8005\u5728\u7701\u6642\u65b9\u9762\u7684\u4e0b\u4e00\u500b\u91cd\u5927\u98db\u8e8d\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u70ba\u4ec0\u9ebc\u7bc0\u7701\u6642\u9593\u5982\u6b64\u91cd\u8981<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u6a5f\u5668\u4eba\u8b93\u60a8\u53ef\u4ee5\u4e26\u884c\u5de5\u4f5c\uff0c\u4ee5\u66f4\u5feb\u5b8c\u6210\u66f4\u591a\u5de5\u4f5c\u2014\u2014\u5c24\u5176\u662f\u914d\u5099\u71b1\u5faa\u74b0\u5100\u7684\u6a5f\u5668\u4eba\uff0c\u5b83\u5011\u53ef\u4ee5\u8655\u7406\u66f4\u9577\u7684\u5de5\u4f5c\u6d41\u7a0b\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7b2c\u4e00\u7a2e\u7bc0\u7701\u6642\u9593\u7684\u65b9\u5f0f\u662f\u57fa\u672c\u7684\u4e00\u5c0d\u4e00\u4efb\u52d9\u5b8c\u6210\u7684\u597d\u8655\u3002\u5982\u679c\u4e00\u500b\u4eba\u53ef\u4ee5\u5728\u4e00\u5c0f\u6642\u5167\u6e96\u5099 100 \u500b\u6a23\u672c\uff0c\u4e26\u5728\u4e00\u5c0f\u6642\u5167\u56de\u8986 10 \u5c01\u96fb\u5b50\u90f5\u4ef6\uff0c\u90a3\u9ebc\u4ed6\u5011\u9700\u8981 2 \u5c0f\u6642\u4f86\u5b8c\u6210\u9019\u5169\u9805\u4efb\u52d9\u3002\u4f46\u5982\u679c\u4ed6\u5011\u8b93\u6a5f\u5668\u4eba\u9032\u884c\u6a23\u54c1\u6e96\u5099\uff0c\u4ed6\u5011\u53ef\u4ee5\u5728 1 \u5c0f\u6642\u5167\u5b8c\u6210\u9019\u5169\u9805\u4efb\u52d9\u2014\u2014\u9019\u662f\u4e00\u7a2e\u76f8\u7576\u7c21\u55ae\u7684\u63d0\u9ad8\u751f\u7522\u7387\u7684\u65b9\u6cd5\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u4f46\u662f\uff0c\u7576\u60a8\u8003\u616e\u66f4\u9577\u7684\u6642\u9593\u6bb5\u6642\uff0c\u7bc0\u7701\u7684\u6642\u9593\u6703\u500d\u589e\u3002\u5047\u8a2d\u60a8\u6709 600 \u500b\u6a23\u672c\u9700\u8981\u6e96\u5099\uff0c\u4ee5\u6bcf\u5c0f\u6642 100 \u500b\u6a23\u672c\u7684\u76f8\u540c\u901f\u5ea6\u9032\u884c\u6a5f\u5668\u4eba\u904b\u884c\u3002\u9084\u6709\u4e00\u500b\u516d\u5c0f\u6642\u7684\u6703\u8b70\uff0c\u60a8\u6709\u6a5f\u6703\u51fa\u5e2d\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u60a8\u53ef\u4ee5\u81ea\u52d5\u6e96\u5099\u6a23\u54c1\u4e26\u53c3\u52a0\u6703\u8b70\u55ce\uff1f<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7406\u8ad6\u4e0a\uff0c\u7576\u7136\u53ef\u4ee5\uff1a6 \u5c0f\u6642 x 100 \u500b\u6a23\u672c\/\u5c0f\u6642 = 600 \u500b\u6a23\u672c\u3002\u4f46\u662f\u8ab0\u4f86\u88dd\u8f09\u548c\u5378\u8f09\u6a5f\u5668\u4eba\uff1f\u8ab0\u4f86\u5728\u904b\u884c\u4e4b\u9593\u8a2d\u5b9a\u81ea\u52d5\u5316\uff1f\u4f60\u53ef\u4ee5\u81ea\u52d5\u5316\u6240\u6709 6 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\/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><strong>\u684c\u4e0a\u578b\u71b1\u5faa\u74b0\u5100\u5982\u4f55\u70ba\u60a8\u7bc0\u7701\u5927\u91cf\u6642\u9593<\/strong><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5982\u679c\u60a8\u7684\u5be6\u9a57\u5ba4\u5df2\u7d93\u5be6\u73fe\u4e86\u5f9e\u57fa\u56e0\u5206\u578b\u548c\u5b9a\u5e8f\u5230\u514b\u9686\u548c\u57fa\u56e0\u7de8\u8f2f\u7b49\u5de5\u4f5c\u6d41\u7a0b\u7684\u81ea\u52d5\u5316\uff0c\u90a3\u9ebc\u5728\u6db2\u9ad4\u8655\u7406\u6a5f\u5668\u4eba\u4e0a\u6dfb\u52a0\u4e00\u53f0\u684c\u4e0a\u578b\u71b1\u5faa\u74b0\u5100\u53ef\u4ee5\u5c07\u60a8\u7684\u7a7a\u9592\u6642\u9593\u589e\u52a0 3 \u500d\u3002\u5145\u5206\u5229\u7528\u6574\u5408\u71b1\u5faa\u74b0\u5100\u7684\u5354\u8b70\u975e\u5e38\u8907\u96dc\uff0c\u6d89\u53ca\u5b75\u80b2\u6216 PCR \u6b65\u9a5f\u4e4b\u524d\u548c\u4e4b\u5f8c\u7684\u591a\u500b\u5b50\u5354\u8b70\u3002\u8b93\u6211\u5011\u4ee5\u6b21\u4e16\u4ee3\u5b9a\u5e8f (NGS) \u6587\u5eab\u88fd\u5099\u5354\u8b70\u70ba\u4f8b\u3002\u900f\u904e\u50c5\u81ea\u52d5\u5316\u5de5\u4f5c\u6d41\u7a0b\u4e2d\u7684\u79fb\u6db2\u548c\u78c1\u73e0\u8655\u7406\u6b65\u9a5f\uff0c\u60a8\u53ef\u4ee5\u6e1b\u8f15\u7d04 2\/3 \u7684\u5de5\u4f5c\u91cf\u3002\u9019\u5c31\u662f\u9175\u7d20\u88fd\u5099\uff08\u788e\u7247\u5316 + a-tailing \u7b49\uff09\u3001\u6e05\u7406\u3001\u7d22\u5f15\u548c\u6c60\u5316\u6b65\u9a5f\uff0c\u9019\u4e9b\u6b65\u9a5f\u53ef\u4ee5\u7531\u5e36\u6709\u78c1\u6027\u6a21\u7d44\u7684 OT-2 \u6a5f\u5668\u4eba\u5b8c\u6210\u3002\u5982\u679c\u6a5f\u5668\u4eba\u7684\u7532\u677f\u4e0a\u6c92\u6709\u71b1\u5faa\u74b0\u5100\uff0c\u90a3\u9ebc\u4e00\u500b\u4eba\uff08\u6216\u53e6\u4e00\u500b\u66f4\u6602\u8cb4\u7684\u6a5f\u5668\u4eba\uff09\u5fc5\u9808\u89aa\u81ea\u5c07\u6e96\u5099\u597d\u7684\u53cd\u61c9\u7269\u79fb\u5165\u71b1\u5faa\u74b0\u5100\u3002\u64f4\u589e\u5b8c\u6210\u5f8c\uff0c\u4ed6\u5011\u9084\u9700\u8981\u89aa\u81ea\u53d6\u56de\u6a23\u672c\u2014\u2014\u5c07\u5b83\u5011\u5f9e\u71b1\u5faa\u74b0\u5100\u4e0a\u53d6\u4e0b\uff0c\u7136\u5f8c\u653e\u56de\u6a5f\u5668\u4eba\u4e0a\uff0c\u9032\u884c\u6700\u5f8c\u7684\u78c1\u6027\u548c\u79fb\u6db2\u6b65\u9a5f\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p><\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u539f\u6587\u7db2\u5740\uff1a<strong><a href=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2023\/11\/How-To-Save-Time-In-The-Lab-With-An-Automated -On-Deck-Thermocycler.pdf\" target=\"_blank\" 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