{"id":2911,"date":"2024-06-14T22:00:22","date_gmt":"2024-06-14T14:00:22","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=2911"},"modified":"2024-09-03T11:14:49","modified_gmt":"2024-09-03T03:14:49","slug":"ot-2hbsthp-1xbgyhqspdzdcd","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/ot-2hbsthp-1xbgyhqspdzdcd\/","title":{"rendered":"OT-2\u6d77\u5831\u4e0aTHP-1\u7d30\u80de\u904e\u6c27\u5316\u6c2b\u6c34\u5e73\u7684\u81ea\u52d5\u6e2c\u5b9a"},"content":{"rendered":"\n
\u6e2c\u5b9aOT-2\u4e0aTHP-1\u7d30\u80de\u4e2d\u904e\u6c27\u5316\u6c2b\u6c34\u5e73\u7684\u81ea\u52d5\u5316\u5206\u6790<\/p>\n\n\n\n
\u74e6\u8607\u9054SNair\u535a\u58eb.1\u91d1\u7d0d\u88e1\u00b7\u6c83\u68ee\u535a\u58eb11.Optrins\u5be6\u9a57\u5ba4\u516c\u53f8\u3002<\/p>\n\n\n\n
\u4ecb\u7d39<\/strong><\/p>\n\n\n\n \u6c99\u6842\u6cb9\u904e\u6c27\u5316\u6c2b\uff08H2O2)\u4e3b\u8981\u8207\u7d30\u80de\u640d\u50b7\u6709\u95dc\uff1b\u7136\u800c\uff0c\u91cd\u65b0\u751f\u6210\u767e\u7f8e\u5206\u7684\u7814\u7a76\u8868\u660e\uff0c\u7d30\u80de\u91cb\u653e\u4f4e\u6c34\u5e73\u7684H2O2\u4f5c\u70ba\u6b63\u5e38\u7d30\u80de\u9593\u7684\u4e00\u90e8\u5206\u9ad8\u983b\u7387\u591a\u8def\u901a\u4fe1\u904e\u6c27\u5316\u6c2b\u7684\u8f49\u904b\u3001\u651d\u53d6\u548c\u91cb\u653e\u7684\u6a5f\u5236\u751f\u7269\u5b78\u6548\u61c9\u76ee\u524d\u9084\u4e0d\u70ba\u4eba\u6240\u77e5\uff0c\u4f46\u5c0d\u764c\u75c7\u3001\u5e79\u7d30\u80de\u6709\u91cd\u8981\u610f\u7fa9\u7d30\u80de\u548c\u8001\u5316\u3002\u96d9\u806f\u9ad4\u7d05\u8272\u8a66\u5291\u662f\u975e\u87a2\u5149\u7684\u57fa\u65bc10-\u4e59\u91af3,7\u4e8c\u7fa5\u57fa\u82ef\u6c27\u60e1\u55ea\u7684\u6c27\u5316\uff0c\u6e2c\u5b9a\u539f\u7406\u7c21\u55ae\u5b83\u662f\u7531\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08HRP\uff09\u50ac\u5316\u7684\uff0c\u5728H2O2\u7522\u751f\u4e00\u500b\u7d05\u8272\u87a2\u5149\u7522\u7269\uff0c\u9593\u82ef\u4e8c\u915a\uff0c\u5316\u5b78\u8a08\u91cf\u6bd4\u70ba1\uff1a1\u3002\u6a39\u8102\u6700\u7d42\u7522\u7269\u5177\u6709\u8f03\u9ad8\u7684\u6d88\u5149\u4fc2\u6578\u9019\u662f\u4e00\u7a2e\u8d85\u9748\u654f\u7684\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u53ea\u670910\u76ae\u6469\u723e\u7684H2O2\u5728\u4e00\u500b100 \u03bcL\u7684\u6372\u4e2d\uff1b1\u00d710\u20135U\/mL\u7684HRP\u3002\u9019\u4e9b\u8b80\u6578\u53ef\u4ee5\u7528\u5206\u5149\u5149\u5ea6\u6cd5\u6e2c\u91cf\uff0c\u4e5f\u53ef\u4ee5\u7528\u87a2\u5149\u8b80\u6578\u6cd5\u6e2c\u91cf\u4f7f\u7528\u9176\u6a19\u5100\uff0c\u6fc0\u767c530 nm\uff0c\u767c\u5c04\u6ce2590 nm\u3002<\/p>\n\n\n\n \u6750\u6599\u8207\u65b9\u6cd5<\/strong><\/p>\n\n\n\n OT-2\uff0cP300\u55aeGEN2\u79fb\u6db2\u7ba1\uff0cP20\u55aeGEN2\u79fb\u6db2\u7ba1\u6eab\u5ea6\u6a21\u7d44\u8a2d\u5b9a\u70ba23\u00b0C\uff0c\u5728\u5354\u8b70\u7d50\u675f\u6642\u8a2d\u5b9a\u70ba37\u00b0C\u3002 THP-1\u5de8\u566c\u7d30\u80de\u6a23\u7d30\u80de\uff08ATCC\uff0cManassas\uff0cVA\uff0cUSA\uff09(No\u3002TIB-202)\u7121\u915a\u7d05RPMI 1640\u57f9\u990a\u57fa\uff08\u71b1\u8cbb\u96ea\u79d1\u5b7811835030\uff09\u4f5b\u6ce212-\u8089\u8373\u853b\u917813-\u918b\u9178\u916f\uff08 PMA\uff09\uff08Sigma Aldrich P1585-1MG\uff09\u80ce\u725b\u8840\u6e05\uff08\u80ce\u725b\u8840\u6e05\uff09\u672a\u71b1\u6ec5\u6d3b\uff08ATCC\uff0cCat No. 30-2020\uff09Amplex\u7d05\u8272\u6aa2\u6e2c\u8a66\u5291\u76d2(Cat No.A22188)\uff0c\u5305\u62eca) 5X\u53cd\u61c9\u7de9\u885d\u6db2b) HRP c) DMSO d) Amplex\u7d05\u8272\u8a66\u5291e) 3% H2O2a)\u8996\u4e2d\u5fc396\u904e\u6ffe\u5668\u5c16\u7aef\u67b6200\u03bcL b) 96\u904e\u6ffe\u5668\u5c16\u7aef\u67b620\u03bcL c)\u8996\u4e2d\u5fc310\u7ba1\u67b6\u8207\u7375\u9df94 x 50 mL\uff0c6 x15mL\u9310\u5f62d)\u8996\u4e2d\u5fc324\u7ba1\u67b6\u8207\u57c3\u5f6d\u591a\u592b1.5 mL\u5b89\u5168\u9396\u6263\u5e3d\uff08\u5169\uff09<\/p>\n\n\n\n