{"id":3073,"date":"2024-06-29T11:09:23","date_gmt":"2024-06-29T03:09:23","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=3073"},"modified":"2024-09-04T18:00:23","modified_gmt":"2024-09-04T10:00:23","slug":"shiyong-ot-2-zdhyypt","status":"publish","type":"knowledge2","link":"https:\/\/opentrons.com.cn\/zh-TW\/knowledge2\/shiyong-ot-2-zdhyypt\/","title":{"rendered":"\u4f7f\u7528 OT-2 \u81ea\u52d5\u5316\u79fb\u6db2\u5e73\u53f0\u9032\u884c\u9ad8\u6548\u80fd\u6838\u9178\u8403\u53d6"},"content":{"rendered":"<!-- wp:paragraph -->\n<p>\u61c9\u7528\u6982\u8ff0\u96a8\u8457\u4e0d\u65b7\u9032\u6b65\u7684\u6280\u8853\u63a8\u52d5\uff0c\u73fe\u4ee3\u5206\u5b50\u751f\u7269\u5b78\u6301\u7e8c\u53d6\u5f97\u842c\u773e\u77da\u76ee\u7684 \u7a81\u7834\u548c\u767c\u5c55\uff0c\u5305\u62ec\u66f4\u5927\u898f\u6a21\u7684\u7814\u7a76\u3001\u66f4\u5f37\u5927\u7684\u6cbb\u7642\u65b9\u6cd5\u4ee5\u53ca\u5168\u65b0 \u7684\u7814\u7a76\u554f\u984c\u3002\u7136\u800c\uff0c\u5118\u7ba1\u6838\u9178\u8403\u53d6\u9019\u9805\u6280\u8853\u662f\u5206\u5b50\u751f\u7269\u5b78\u5f97\u4ee5\u9032 \u6b65\u7684\u4e00\u9805\u91cd\u8981\u4e14\u5bc6\u4e0d\u53ef\u5206\u7684\u5be6\u9a57\u74b0\u7bc0\uff0c\u79d1\u7814\u4eba\u54e1\u4ecd\u5728\u6cbf\u7528\u8457\u5e7e \u5341\u5e74\u524d\u5197\u9577\u800c\u7e41\u7463\u7684\u7a0b\u5e8f\u9032\u884c\u6838\u9178\u63d0\u53d6\u3002\u5728\u9019\u6a23\u7684\u56f0\u5c40\u4e4b\u4e0b\uff0c\u81ea \u52d5\u5316\u89e3\u6c7a\u65b9\u6848\u61c9\u904b\u800c\u751f\u3002\u73fe\u4ee3\u81ea\u52d5\u5316\u900f\u904e\u5f15\u5165\u5177\u5099\u7a69\u5b9a\u6027\u548c\u9ad8\u6548 \u904b\u4f5c\u512a\u52e2\u7684\u6a5f\u5668\u8a2d\u5099\u5b8c\u6210\u6a19\u6e96\u5316\u6d41\u7a0b\u9ad8\u6548\u81ea\u52d5\u904b\u884c\uff0c\u81ea\u52d5\u5316\u6838\u9178 \u8403\u53d6\u5c07\u4f7f\u751f\u7269\u5be6\u9a57\u5ba4\u820a\u6709\u7684\u5de5\u4f5c\u65b9\u5f0f\u8fce\u4f86\u73fe\u4ee3\u5316\u3002\u70ba\u4e86\u771f\u6b63\u610f\u7fa9 \u4e0a\u5e6b\u52a9\u79d1\u7814\u4eba\u54e1\u9054\u5230\u9ad8\u6548\u7814\u767c\u6216\u751f\u7522\u7684\u76ee\u7684\uff0c\u81ea\u52d5\u5316\u7cfb\u7d71\u9700\u8981\u9054 \u5230\u751a\u81f3\u8d85\u8d8a\u9ad8\u6280\u80fd\u6280\u8853\u4eba\u54e1\u7684\u8868\u73fe\uff0c\u70ba\u4e0b\u6e38\u5de5\u4f5c\u6d41\u7a0b\u63d0\u4f9b\u9ad8\u54c1\u8cea\u7684\u6a23\u672c\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u672c\u7814\u7a76\u65e8\u5728\u6e2c\u8a66Opentrons OT-2\u6838\u9178\u5de5\u4f5c\u7ad9\u4f5c\u70ba\u6709\u6548\u81ea\u52d5\u5316 \u78c1\u73e0\u57fa\u56e0\u8403\u53d6\u5de5\u5177\u7684\u6027\u80fd\u3002\u7814\u7a76\u4e2d\u4f7f\u7528\u4e86\u5e7e\u7a2e\u7814\u7a76\u4eba\u54e1\u548c\u81e8\u5e8a\u91ab \u751f\u5e38\u7528\u7684\u5e38\u898b\u6a21\u677f\uff1a\u4eba\u985e\u553e\u6db2\u3001\u4eba\u985e\u53e3\u8154\u62ed\u5b50\u3001\u7d30\u83cc\u57f9\u990a\u548c RNA \u75c5\u6bd2\u3002\u540c\u6642\uff0c\u4f7f\u7528\u4e86\u696d\u754c\u5e38\u898b\u7684\u4e3b\u6d41\u8a66\u5291\u54c1\u724c\u3002\u900f\u904e\u7522 \u91cf\u3001CV \u503c\u548cqPCR \u6578\u64da\u9032\u884c\u6548\u80fd\u8a55\u4f30\uff0c\u5be6\u9a57\u6578\u64da\u5c07\u8207\u9ad8\u6280\u80fd\u6280\u8853\u4eba\u54e1\u7684\u624b\u52d5\u64cd\u4f5c\u7d50\u679c\u9032\u884c\u6bd4\u8f03\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7d93\u904e\u7814\u7a76\u6e2c\u8a66\uff0cOT-2 \u8868\u73fe\u512a\u7570\u3002\u4f7f\u7528OT-2 \u9032\u884c\u63d0\u53d6\u7684\u5be6\u9a57\u8b8a\u7570 \u6027\u964d\u4f4e\uff0c\u540c\u6642\u5fa9\u73fe\u7387\u63d0\u9ad8\uff0c\u4e26\u4e14\u80fd\u5728\u8f03\u4f4e\u7684\u6210\u672c\u548c\u66f4\u5feb\u7684\u5468\u8f49\u6642 \u9593\u4e0b\u63d0\u4f9b\u4e86\u985e\u4f3c\u7684\u7522\u91cf\u3002\u91cd\u8981\u7684\u662f\uff0c\u9019\u4e9b\u6548\u80fd\u6539\u9032\u548c\u6210\u672c\u7bc0\u7701\u53ef \u4ee5\u96a8\u8457\u541e\u5410\u91cf\u7684\u589e\u52a0\u800c\u63d0\u5347\u3002\u56e0\u6b64\uff0c\u8a72\u6e2c\u8a66\u8868\u660e\uff0c\u4f7f\u7528 OT-2 \u9032 \u884c\u6838\u9178\u8403\u53d6\u81ea\u52d5\u5316\u53ef\u4ee5\u4f7f\u5be6\u9a57\u6d41\u7a0b\u9ad8\u6548\u81ea\u52d5\u5316\uff0c\u8b93\u81ea\u52d5\u5316\u6838\u9178\u8403\u53d6\u80fd\u5920\u6eff\u8db3\u9ad8\u7aef\u5206\u5b50\u751f\u7269\u5b78\u7684\u9ad8\u6027\u80fd\u6a19\u6e96\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7814\u7a76\u6750\u6599\u8207\u65b9\u6cd5\u4eba\u985e\u553e\u6db2\uff1a \u5f9e\u533f\u540d\u6350\u8d08\u8005\u6536\u96c6\u65b0\u9bae\u553e\u6db2\u4e26\u5132\u5b58\u572815 mL \u9310\u5f62\u7ba1\u4e2d\u76f4\u81f3\u4f7f\u7528\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u53e3\u8154\u62ed\u5b50\uff1a\u4f7f\u752820 mm break point (Zymo@) \u53e3\u8154\u63a1\u96c6\u62ed\u5b50\u5c0d\u533f\u540d \u5fd7\u9858\u8005\u9032\u884c\u63a1\u6a23\uff0c\u4e26\u5132\u5b58\u65bc\u57281 mL Zymo DNA\/RNA \u4fdd\u8b77\u6db2\u4e2d\uff0c\u5ba4\u6eab\u4fdd\u5b58\u904b\u8f38\uff0c<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7d30\u83cc\u57f9\u990a\uff1a \u4f7f\u7528 One shot\u00b0TOP10 \u88fd\u5099\u611f\u53d7\u614b\u5927\u8178\u687f\u83cc (Thermo Fisher Scientific*) \u572837\u00b0\u2103,225 rpm \u632f\u76ea\u689d\u4ef6\u4e0b\u5728\u6eb6\u83cc\u6db2\u4e2d\u57f9\u990a\u904e \u591c\u3002\u4f7f\u7528\u524d\uff0c\u6bcf\u500b\u6a23\u54c1\u4ee52000 rpm \u96e2\u5fc31.5 \u5206\u9418\u3002\u79fb\u9664\u4e0a\u6e05\u6db2\uff0c\u6c89\u6fb1\u7269\u88ab\u91cd\u65b0\u61f8\u6d6e\u65bc\u7b49\u9ad4\u7a4d\u7684\u51b7\u78f7\u9178\u9e7d\u7de9\u885d\u6db2\u4e2d\u3002\u6b64\u5916\uff0c\u5c0d\u65bc\u4e73\u9178\u687f\u83cc\u6a23\u54c1\uff0c\u6bcf\u500b\u6a23\u54c1\u4f7f\u7528\u7d041\u00d7107\u62f7\u8c9d\/\u03bcL\u7684 Lactobacillus plantarum(ATCC\u00b08014MINIPACKTM)\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u75c5\u6bd2\uff1a\u7531\u65bc2020\u5e743\u6708COVID-19 \u5168\u7403\u5927\u6d41\u884c\u958b\u59cb(1),\u75c5\u6bd2 RNA \u8a66\u5291\u76d2\u4e2d\u4f7f\u7528\u4e86\u7684\u5408\u6210 SARS-COV-2 RNA Control 2(Twist Biosci- ences), \u6bcf\u500b\u6a23\u54c110\u62f7\u8c9d\/\u03bcL\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5167\u90e8\u5c0d\u7167\uff1a\u4f9d\u7167 Panpradist\u3001Nuttada \u7b49\u4eba(2)\u4e2d\u6240\u8ff0\u4f7f\u7528\u548c\u5275\u5efa\u5408 \u6210\u9f3b\u6a23\u57fa\u8cea(SNM)\u3002 SNM \u7684\u914d\u65b9\u70ba\uff1a110 mM NaCl\u30011%w\/v\u4f86\u81ea\u8c6c\u80c3\u985e\u578bI\u7684\u9ecf\u86cb\u767d(Sigma M2378-100G)\u548c10 \u03bcg\/mL w\/v \u4eba\u985e\u57fa\u56e0\u7d44DNA(Coriell NA12878), \u6df7\u5408\u9ad4\u7a4d\u70baTE\/SNM\u768490%\u3002\u6bcf\u500b\u75c5\u6bd2\u6a23\u672c\u4f7f\u752850 pg\/\u03bcL\u7684 SNM\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u8a66\u5291\u76d2\uff1a\u672c\u7814\u7a76\u4e2d\u9078\u64c7\u4e86\u4f86\u81ea\u4e0d\u540c\u54c1\u724c\u7684 DNA\u3001RNA \u548c\u75c5\u6bd2 RNA\u8403\u53d6\u8a66\u5291\u76d2(\u53c3\u898b\u88681)\u3002\u7e3d\u5171\u6aa2\u9a57\u4e867\u500b\u8a66\u5291\u54c1\u724c\u768413\u7a2e\u8a66\u5291\u76d2\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3074,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-122.png\" alt=\"\u672c\u7814\u7a76\u6240\u68c0\u9a8c\u7684\u63d0\u53d6\u8bd5\u5242\" class=\"wp-image-3074\"><figcaption class=\"wp-element-caption\"><strong>\u88681:\u672c\u7814\u7a76\u6240\u6aa2\u9a57\u7684\u8403\u53d6\u8a66\u5291\u3002 <\/strong>\u5728\u4e03\u500b\u77e5\u540d\u8a66\u5291\u54c1\u724c\u4e2d\uff0c\u6709\u5169\u7a2e\u200b\u200bDNA\u8403\u53d6\u8a66\u5291\u76d2\u3001\u56db\u7a2eRNA\u8403\u53d6\u8a66\u5291\u76d2\u548c\u4e03\u7a2e\u75c5\u6bd2RNA\u8403\u53d6\u8a66\u5291\u76d2\uff0c\u5747\u53ef\u5728OT-2\u4e0a\u81ea\u52d5\u5316\u64cd\u4f5c\u3002<\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u8403\u53d6\uff1a \u5728\u81ea\u52d5\u5316\u6aa2\u6e2c\u4e4b\u524d\uff0c\u6211\u5011\u5c0d\u6bcf\u500b DNA \u548c RNA \u8403\u53d6\u8a66\u5291\u53ca \u6a21\u677f\u9032\u884c\u4e86\u624b\u52d5\u6e2c\u8a66\uff0c\u4ee5\u53d6\u5f97\u624b\u52d5\u6e2c\u8a66\u57fa\u7dda\u6fc3\u5ea6\u3002\u6bcf\u500b\u6a21\u677f\u5728\u6bcf \u7a2e\u8a66\u5291\u4e2d\u9032\u884c7\u6b21\u91cd\u8907\u6e2c\u8a66\uff0c\u4e26\u914d\u50991 \u500b\u9670\u6027\u5c0d\u7167\u6a23\u672c\u3002\u672c\u6b21\u7814 \u7a76\u5728 Opentrons \u5e73\u53f0\u4e0a\u9032\u884c\u4e86\u81ea\u52d5\u5316\u6838\u9178\u8403\u53d6\u3002\u5c0d\u65bc DNA \u548c RNA \u8403\u53d6\u8a66\u5291\uff0c\u6bcf\u500b\u6a23\u672c\u9032\u884c\u4e867 \u6b21\u8403\u53d6\u3002\u5c0d\u65bc\u75c5\u6bd2RNA \u8403\u53d6\u8a66 \u5291\u76d2\uff0c\u5305\u62ec\u4e8622 \u500b\u5177\u6709\u76f8\u540c\u7684 SARS-CoV-2 \u548c SNM \u6a21\u677f\u7684\u6a23\u672c \u672c\u3002\u6bcf\u500b OT-2 \u904b\u884c\u4e2d\u90fd\u5305\u62ec1\u500b\u9670\u6027\u5c0d\u7167\u6a23\u672c(nuclease-free H2O) \u3002 OT-2 \u7684\u8a2d\u5099\u7d44\u88dd\u4e86 GEN2 \u78c1\u73e0\u7d14\u5316\u6a21\u7d44\u3001p300 \u591a\u901a\u9053 \u79fb\u6db2\u7ba1\u548c\u9078\u8cfc\u914d\u7684 GEN2 \u6eab\u63a7\u6a21\u7d44\uff0c\u5c0d\u65bc RNA \u5be6\u9a57\u4f86\u8aaa\uff0c\u70ba\u78ba\u4fdd \u66f4\u597d\u7684\u5be6\u9a57\u6548\u679c\uff0c\u5efa\u8b70\u914d\u5099\u6eab\u63a7\u6a21\u7d44\u3002\u6839\u64da\u4e0d\u540c\u7684\u8a66\u5291\u76d2\uff0c\u9700\u8981 \u4f7f\u7528\u5927\u7d0414\u76d2300 \u03bcL \u5438\u982d\uff0c1 \u500b NEST \u7684\u55ae\u5b54\u5132\u6db2\u5668\uff0c1 \u500bNEST 2 mL\u6df1\u5b54\u677f\uff0c2\u500b NEST12 \u5b54\u5132\u6db2\u69fd\u548c1\u500bPCR \u5b54\u677f\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5f15\u5b50\uff1a\u75be\u75c5\u7ba1\u5236\u8207\u9810\u9632\u4e2d\u5fc3(CDC)\u7684\u5f15\u5b50\u548c\u63a2\u91dd\uff1a<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>2019-nCoV_N1-F(GACCCCAAAATCAGCGAAAT)2019-nCoV_N1-R (TCTGGTTACTGCCAGTTGAATCTG)2019-nCoV_N1-P(FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1)2019-nCoV_N2-F(TTACAAACATTGGCCGCAAA)2019-nCoV_N2- R(GCGCGACATTCCGAAGAA)2019-nCoV_N2-P(FAMACAATTTGCCCCCAGCGCTTCAG-BHQ1)RP-F(AGATTTGGACCTGCGAGCG),RP-R(GAGCGGCTGTCTCCACAAGT)RP-P (FAM-TTCTGACCTGAAGGCTCTGCGCG-BHQ1)<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>CDC \u5f15\u5b50\u548c\u63a2\u91dd\u8a02\u8cfc\u81ea\u5f9eIntegrated DNA Technologies\u516c\u53f8\uff0c\u53c8\u7a31IDT (2019-nCoV CDC EUA\u8a66\u5291\u76d2)\u3002 N1\u3001N2 \u548cRNase P\u4ee5CDC \u5efa\u8b70\u7684\u6fc3\u5ea6\u9032\u884c\u9810\u6df7\u3002 16s \u5f15\u5b50\uff1aForward (CCTATAAGACTGGGATAACTTCGGG)Reverse (CTTTGAGTTTCAACCTTGCGGTCG)16s \u5f15\u5b50\u5f9eIDT \u8a02\u8cfc\uff0c\u65bcIDTE 1XTE \u6eb6\u6db2 pH:8.0(DT) \u4e2d\u91cd\u61f8\uff0c\u7a00\u91cb\u81f310uM \u7684\u5de5\u4f5c\u6fc3\u5ea6\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5b9a\u91cf\u6e2c\u91cf\uff1a DNA \u548c RNA \u6a23\u672c\u7684\u6fc3\u5ea6\u53ef\u4ee5\u4f7f\u7528 Qubit 4.0 FluorometerM(Thermo Fisher)\u9032\u884c\u5b9a\u91cf\u6e2c\u91cf\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u6b64\u5916\uff0c\u6240\u6709\u6a23\u672c\u5747\u5728 PCR max ECO48 \u5be6\u6642 PCR \u7cfb\u7d71\u4e0a\u9032\u884c qPCR \u5b9a \u91cf\u3002 RNA \u6a23\u672c\u4f7f\u7528Luna\u00b0\u901a\u7528\u63a2\u91dd\u4e00\u6b65\u5f0f RT-qPCR \u8a66\u5291\u76d2(NEB) \u9032\u884c \u6e2c\u8a66\uff0c\u6fc3\u5ea6\u7531 Lista \u3001Maria Jose \u7b49\u4eba\u6e2c\u8a66(3)\u3002 \u9032\u884c\u4ee5\u4e0b\u7a0b\u5e8f\uff1a\u5728 55\u2103\u4e0b\u9032\u884c\u9006\u8f49\u930410\u5206\u9418\u3002\u521d\u59cb\u8b8a\u6027\u572895\u2103\u4e0b\u9032\u884c1 \u5206\u9418\uff0c\u7136\u5f8c\u9032 \u884c50\u500b\u5faa\u74b0\uff0c\u5373\u572895\u2103\u4e0b\u8b8a\u602710\u79d2\uff0c\u4e26\u572860\u2103\u4e0b\u9000\u706b30\u79d2\u3002\u4f7f\u7528 Luna Universal qPCR Master Mix (NEB)\u4ee5\u88fd\u9020\u5546\u5efa\u8b70\u7684\u6fc3\u5ea6\u5c0dDNA\u6a23 \u54c1\u9032\u884c\u6e2c\u8a66\u3002\u4f7f\u7528\u4ee5\u4e0b\u7a0b\u5e8f\uff1a\u521d\u59cb\u8b8a\u6027\u572895\u2103\u4e0b\u9032\u884c60\u79d2\uff0c\u96a8\u5f8c\u8b8a\u6027\u548c\u5ef6\u4f38\u5206\u5225\u572895\u2103\u548c60\u2103\u4e0b\u9032\u884c15\u79d2\u548c30\u79d2\uff0c\u6301\u7e8c40\u500b\u5faa\u74b0\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5be6\u9a57\u7d50\u679c\u900f\u904e qPCR \u548c\u87a2\u5149\u8a08\u5206\u6790\u6e2c\u91cf\uff0c OT-2 \u8403\u53d6\u7684\u6838\u9178\u6578\u91cf\u63a5 \u8fd1\uff0c\u6838\u9178\u6a23\u672c\u7684 \u4e00 \u81f4\u6027\u66f4\u9ad8\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>OT-2 \u7d93\u904e\u4e86\u7b26\u5408EUA \u6a19 \u6e96 \u7684\u6027\u80fd\u6e2c\u8a66\uff0c\u53ef\u4ee5\u8207\u591a\u7a2eqPCR \u6838\u63d0\u53d6\u8a66\u5291\u76d2 \u4e00 \u8d77\u4f7f\u7528\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u4f7f\u7528\u4e5d\u7a2e\u5e38\u7528\u7684\u8403\u53d6\u8a66\u5291\u76d2\u8655\u7406\u4eba\u985e\u553e\u6db2\u548c\u690d\u7269\u4e73\u687f\u83cc\u7d30\u83cc\u57f9\u990a \u7269\uff0c\u4e26\u4f7f\u7528\u5169\u7a2e\u5e38\u898b\u7684 qPCR \u6a19\u9776\u9032\u884c qPCR \u5206\u6790\uff1a RNase P \u7528\u65bc \u553e\u6db2\u6a23\u672c\uff0c16s \u7528\u65bc\u7d30\u83cc\u6a23\u672c\u3002\u4f7f\u7528\u516d\u500b\u8a66\u5291\u76d2\u8655\u7406\u5927\u8178\u687f\u83cc\u57f9\u990a\u7269\u4e26\u4f7f\u7528\u87a2\u5149\u5206\u6790\u9032\u884c\u5206\u6790\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7522\u91cf\u900f\u904eqPCR \u4e2d\u7684\u5faa\u74b0\u95be\u503c(Ct) \u548c\u87a2\u5149\u8a08\u4e2d\u6240\u5f97\u7684ng\/\u03bcL\u9032\u884c\u8a55\u4f30\u3002 \u4e00\u81f4\u6027\u5247\u900f\u904eCt\u503c\u7684\u8b8a\u7570\u4fc2\u6578 (CV) \u9032\u884c\u8a55\u4f30\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>Ct\u503c\u53cd\u6620\u4e86\u6a23\u54c1\u8de8\u8d8a\u9ad8\u65bc\u80cc\u666f\u8a0a\u865f\u7684\u95be\u503c\u6240\u9700\u7684\u8907\u88fd\u9031\u671f\u91cf\uff0c\u56e0\u6b64 \u5b83\u5011\u50b3\u9054\u4e86\u6578\u91cf\u7684\u5012\u6578\u6e2c\u91cf\u3002\u5c0d\u65bc\u9019\u4e9b\u76ee\u6a19\uff0c\u5728\u6b64\u985e\u6a23\u54c1\u4e2d\uff0c25-35\u7684 Ct \u503c\u8f03\u70ba\u53ef\u9760\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u900f\u904e\u6240\u6709\u8403\u53d6\u8a66\u5291\u76d2\u9032\u884c\u6aa2\u9a57\u6e2c\u8a66\uff0c\u4f7f\u7528OT-2\u81ea\u52d5\u5316\u7cfb\u7d71\u9032\u884c\u6838\u9178 \u8403\u53d6\u7d14\u5316\u53ef\u4ee5\u5f97\u5230\u5177\u6709\u8f03\u4f4eCV \u548c\u53ef\u6bd4\u8f03\u7522\u91cf\u7684Ct\u503c(\u898b\u57161\u548c\u88682)\u3002\u719f\u7df4\u7684\u6280\u8853\u4eba\u54e1\u548cOT-2\u56de\u5831\u7684\u5e73\u5747Ct\u503c\u70ba25-35\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u624b\u52d5\u8655\u7406\u7684CV \u503c\u70ba1.41-4.8, OT-2 \u7684CV \u503c\u70ba1.2-2.6\u3002\u719f\u7df4\u7684\u6280\u8853 \u4eba\u54e1\u548cOT-2\u63d0\u4f9b\u4e86\u53ef\u6bd4\u8f03\u4e14\u4e00\u81f4\u768424-26 ng\/\u03bcL\u7684\u7522\u91cf\u3002\u9019\u4e9b\u7d50\u679c\u8868\u660e\uff0c OT-2\u80fd\u5920\u5728\u63d0\u9ad8\u4e00\u81f4\u6027\u548c\u91cd\u8907\u6027\u7684\u540c\u6642\u63d0\u4f9b\u9ad8\u7522\u91cf\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3075,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-123.png\" alt=\"\u663e\u793a\u81ea\u52a8\u63d0\u53d6\u7684\u4ea7\u91cf\u53d8\u5f02\u8f83\u5c0f\" class=\"wp-image-3075\"><figcaption class=\"wp-element-caption\"><strong>\u57161:\u986f\u793a\u81ea\u52d5\u8403\u53d6\u7684\u7522\u91cf\u8b8a\u7570\u8f03\u5c0f\uff0c\u4e14\u8403\u53d6\u7d14\u5316\u7522\u91cf\u53ef\u5ab2\u7f8e\u624b<\/strong>&nbsp;&nbsp;<strong>\u52d5\u8655\u7406\u7684\u7d50\u679c\u3002 <\/strong><\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5728\u8207\u65b0\u51a0\u75c5\u6bd2\u76f8\u95dc\u7684\u6e2c\u8a66\u4e2d\uff0c\u4f7f\u7528\u5408\u6210\u7684 SARS-CoV-2 \u6a23\u672c\u548c\u4e03\u7a2e \u5e38\u7528\u7684\u8403\u53d6\u8a66\u5291\u76d2\u9032\u884c\u4e86qPCR\u3002 \u6211\u5011\u4f7f\u7528\u4e86 SNM \u4e2d\u7684\u4eba\u985e\u57fa\u56e0\u7d44 DNA \u4f5c\u70ba\u5167\u90e8\u5c0d\u7167\uff0c\u4f7f\u7528CDC \u7684 SARS-CoV-2N1 \u548c N2 \u5f15\u5b50\u9032\u884c\u5b9a\u91cf\u5206\u6790\uff0c\u540c\u6642\u4f7f\u7528 CDC \u7684 RP \u5f15\u5b50\u4f5c\u70ba\u967d\u6027\u5c0d\u7167\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u9019\u9805\u6e2c\u8a66\u65e8\u5728\u8a55\u4f30\u4f7f\u7528\u4e0d\u540c\u8403\u53d6\u8a66\u5291\u76d2\u5728 OT-2 \u4e0a\u9032\u884c\u65b0\u51a0\u75c5\u6bd2 qPCR \u5206\u6790\u7684\u6027\u80fd\u3002\u900f\u904e\u4f7f\u7528\u5408\u6210\u7684 SARS-CoV-2 \u6a23\u672c\u4ee5\u53ca\u5167\u90e8\u5c0d \u7167\u548c\u967d\u6027\u5c0d\u7167\uff0c\u53ef\u4ee5\u5224\u65b7 OT-2 \u5728\u6aa2\u6e2c\u75c5\u6bd2\u57fa\u56e0\u7d44\u7684\u6e96\u78ba\u6027\u548c\u53ef\u9760\u6027\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>qPCR \u6027\u80fd\u7684\u8a55\u4f30\u662f\u57fa\u65bc PCR \u6aa2\u6e2c\u7dca\u6025\u4f7f\u7528\u6388\u6b0a (EUA) \u6a19\u6e96\u3002 \u70ba\u4e86\u6eff\u8db3 EUA \u6a19\u6e96\uff0c \u4e00\u500b\u6e2c\u8a66\u5fc5\u9808\u9054\u523095%\u7684\u64f4\u589e\u6548\u679c\uff0c\u4e5f\u5c31\u662f\u8aaa\uff0c\u5728qPCR \u4e2d\uff0c95%\u7684\u6a23\u672c\u5fc5\u9808\u7522\u751f\u53ef\u5075\u6e2c\u7684N1\u548cN2\u8a0a\u865f\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5728\u9019\u7a2e\u6027\u80fd\u8a55\u4f30\u4e2d\uff0c\u6211\u5011\u4e5f\u5c0d qPCR Ct \u503c\u9032\u884c\u4e86\u8a55\u4f30\u3002\u5c0d\u65bc\u9019\u4e9b\u76ee\u6a19\uff0c\u5728\u9019\u985e\u6a23\u672c\u4e2d\uff0c\u6a19\u6e96 Ct\u503c\u61c9\u8a72\u572830-40\u4e4b\u9593\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u6839\u64da\u8868\u683c 3 \u7684\u7d50\u679c\uff0cOT-2 \u5e7e\u4e4e\u5728\u6240\u6709\u8a66\u5291\u76d2\u4e0a\u7684\u8868\u73fe\u90fd\u901a\u904e\u4e86 EUA \u6a19\u6e96\u3002\u9664\u4e86\u5169\u500b\u7531\u65bc\u88c2\u89e3\u7de9\u885d\u6db2\u904e\u5ea6\u9ecf\u7a20\u5c0e\u81f4\u59a8\u7919\u4e86\u8403\u53d6\u904e\u7a0b \u7684\u8a66\u5291\u76d2\u53ea\u670991%\u7684\u6e2c\u8a66\u7d50\u679c\u5408\u683c\u3002\u4e26\u4e14\uff0c\u5728\u6240\u6709\u88ab\u6e2c\u8a66\u7684\u8a66\u5291\u4e2d\uff0c \u6a23\u672c\u7d50\u679c\u5747\u9054\u5230\u4e8631-39\u7684\u6a19\u6e96 Ct \u503c\u3002\u9019\u4e9b\u7d50\u679c\u8868\u660e\uff0cOT-2 \u7cfb\u7d71\u53ef\u4ee5\u8207\u5404\u7a2e\u54c1\u724c\u7684\u8a66\u5291\u914d\u5c0d\u4f7f\u7528\uff0c\u4e26\u9054\u5230\u81e8\u5e8a\u6a19\u6e96\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u70ba\u4e86\u6e2c\u8a66OT-2 \u7cfb\u7d71\u7684\u541e\u5410\u91cf\u548c\u901f\u5ea6\uff0c\u6211\u5011\u4f7f\u7528\u4e8613\u7a2e\u71b1\u9580\u8a66\u5291\u76d2 \u9032\u884c\u4e86RNA \u548cDNA \u8403\u53d6\uff0c\u4e26\u8986\u84cb\u4e86\u4e0d\u540c\u9577\u5ea6\u3002\u70ba\u4e86\u5168\u9762\u8a55\u4f30\u4e0d\u540c\u541e \u5410\u91cf\u4e0b\u7684\u901f\u5ea6\uff0c\u6e2c\u8a66\u4e86\u6bcf\u62798\u300124\u300148\u548c96\u500b\u6a23\u672c\u7684\u60c5\u6cc1\u3002\u4ee5\u5f9e\u539f\u59cb\u6a23\u672c\u5230\u7d14\u5316\u6838\u9178\u6240\u9700\u7684\u6642\u9593\u4f86\u8861\u91cf\u5be6\u9a57\u7d50\u679c\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5728\u9019\u4e9b\u8403\u53d6\u8a66\u5291\u65b9\u6848\u4e2d\uff0cOT-2 \u7cfb\u7d71\u5728\u5404\u7a2e\u8a66\u5291\u76d2\u548c\u9577\u5ea6\u4e0b\u5be6\u73fe\u4e86 \u5feb\u901f\u8403\u53d6\u7684\u7d50\u679c\u3002\u5728\u6700\u77ed\u7684\u5354\u8b70\u4e2d\uff0c\u8655\u74068 \u500b\u6a23\u672c\u53ea\u9700\u898125 \u5206 \u9418\uff0c\u5728\u6700\u9577\u7684\u5354\u8b70\u4e2d\uff0c\u8655\u740696\u500b\u6a23\u672c\u4e0d\u52305\u5c0f\u6642(\u57162)\u3002\u5c0d\u65bc8 \u500b\u6a23\u672c\u7684\u8655\u7406\u6642\u9577\uff0c OT-2 \u572813\u7a2e\u8a66\u5291\u76d2\u4e2d\u5e73\u5747\u8655\u7406\u6642\u9593\u70ba52\u5206 \u9418\u3002\u800c\u572896\u500b\u6a23\u672c\u7684\u60c5\u6cc1\u4e0b\uff0c OT-2 \u572813\u7a2e\u8a66\u5291\u76d2\u4e2d\u5e73\u5747\u8655\u7406\u6642\u9593 \u70ba2\u5c0f\u664245\u5206\u9418\u3002\u9019\u4e9b\u7d50\u679c\u8868\u660e\uff0c OT-2\u7cfb\u7d71\u53ef\u4ee5\u5c07\u5176\u9ad8\u6548\u80fd\u8207\u9ad8\u541e\u5410\u91cf\u7d50\u5408\uff0c\u986f\u8457\u6539\u5584\u63d0\u53d6\u5de5\u4f5c\u6d41\u7a0b\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3076,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-124.png\" alt=\"\u4e0d\u540c\u63d0\u53d6\u8bd5\u5242\u76d2\u6570\u636e\" class=\"wp-image-3076\"><figcaption class=\"wp-element-caption\">\u88682:\u9019\u500b\u8868\u683c\u5c55\u793a\u4e86\u4f7f\u7528\u624b\u52d5\u548c\u81ea\u52d5\u8655\u7406 \u65b9\u6cd5\u9032\u884c\u7684\u4e0d\u540c\u8403\u53d6\u8a66\u5291\u76d2\u5728\u7522\u7387\u548c\u7cbe\u78ba \u6027\u65b9\u9762\u7684\u53ef\u6bd4\u6027\u3002\u8868\u683c\u5de6\u5074\u986f\u793a\u4e86\u5e73\u5747\u8b8a\u5316 \u7570\u4fc2\u6578 (CV) \u548c\u5faa\u74b0\u95be\u503c (Ct)\u3002<\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":3077,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-125.png\" alt=\"\u5c55\u793a\u4e86OT-2 \u81ea\u52a8\u5316\u7684\u5e7f\u6cdb\u9002\u7528\u6027\" class=\"wp-image-3077\"><figcaption class=\"wp-element-caption\">\u88683:\u9019\u500b\u8868\u683c\u8aaa\u660e\u4e86\u4f7f\u7528SARS-CoV-2 \u75c5\u6bd2\u6e2c\u8a66\u7684\u54c1\u7ba1\u898f\u7bc4\uff0c\u5c55\u793a\u4e86OT-2 \u81ea\u52d5\u5316\u7684\u5ee3\u6cdb\u9069\u7528\u6027\u3002\u4f7f\u75287\u500b\u4e0d\u540c\u54c1\u724c\u76847\u7a2e\u75c5\u6bd2\u8a66\u5291\u76d2\u5728OT-2 \u4e0a\u9032\u884c\u81ea\u52d5\u5316\u8655\u7406\u5f8c\uff0c\u6bcf\u7a2e\u8a66\u5291\u76d2 (n=22) \u4e2d\u6709\u8d85\u904e96%\u7684\u6a23\u672c\u5408\u683c\u3002<\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":3078,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-126.png\" alt=\"\u5c55\u793a\u4e86OT-2 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