{"id":3050,"date":"2024-06-28T11:47:40","date_gmt":"2024-06-28T03:47:40","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=3050"},"modified":"2024-09-02T18:30:33","modified_gmt":"2024-09-02T10:30:33","slug":"shiyong-opentrons-ot-2-jxzdhlc","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/zh-TW\/news\/shiyong-opentrons-ot-2-jxzdhlc\/","title":{"rendered":"\u4f7f\u7528Opentrons OT-2\u642d\u914d\u71b1\u632f\u76ea\u5100\u9032\u884c\u4e09\u7a2eELISA\u81ea\u52d5\u5316\u6d41\u7a0b"},"content":{"rendered":"<!-- wp:paragraph -->\n<p>\u4f5c\u8005Boren Lin,PhD,Kinnari Watson,PhD<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u61c9\u7528\u6982\u8ff0\u9175\u7d20\u514d\u75ab\u5206\u6790 (EIA) \u6216\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u6e2c\u5b9a (ELISA) \u662f\u6db2\u9ad4\u6a23\u672c\u4e2d\u6aa2\u6e2c\u76ee\u6a19\u5206\u5b50(\u4f8b\u5982\u6297\u539f)\u7684\u5e38\u7528\u5206\u6790\u5de5\u5177\uff0c\u901a\u5e38\u7528\u65bc\u514d\u75ab\u8a3a\u65b7 \u6216\u7814\u7a76\u3002\u672c\u6e2c\u5b9a\u4e00\u822c\u63a1\u752896 \u5b54\u677f\u683c\u5f0f\uff0c\u4e14\u5de5\u4f5c\u6d41\u7a0b\u901a\u5e38\u5305\u62ec \u8a66\u5291\u8f49\u79fb\u548c\u6df7\u5408\uff0c\u4ee5\u53ca\u4e00\u4e9b\u5728\u6046\u5b9a\u6eab\u5ea6\u4e0b\u7684\u5b75\u80b2\u548c\u6d17\u6ecc\u6b65\u9a5f\u3002\u9019 \u4e9b\u6b65\u9a5f\u5747\u8981\u6c42\u6a23\u672c\u8655\u7406\u9ad8\u5ea6\u4e00\u81f4\u6027\u3002\u70ba\u4e86\u5be6\u73fe\u81ea\u52d5\u5316\uff0c\u6211\u5011\u4f7f\u7528OT-2 \u81ea\u52d5\u5316\u79fb\u6db2\u5de5\u4f5c\u7ad9\u642d\u914d\u71b1\u632f\u76ea\u5100\u7814\u767c\u4e26\u6e2c\u8a66\u4e86ELISA \u5168\u81ea\u52d5\u5316\u7684\u65b9\u6848\uff0c\u8a72\u65b9\u6848\u4f7f\u7528Takara Bio \u516c\u53f8\u7684\u593e\u5fc3\u6cd5EIA \u8a66\u5291\u76d2\u6aa2\u6e2c\u4eba\u985e\u7e96\u9023\u86cb\u767d(FN),Tecan \u7684\u7af6\u722d\u6cd5ELISA \u8a66\u5291\u76d2\u6aa2\u6e2c\u553e\u6db2\u4e2d\u7684\u76ae\u8cea\u9187\uff0c\u4ee5\u53caCell Sciences \u7684\u53e6\u4e00\u7a2e\u7af6\u722d\u6cd5ELISA \u8a66\u5291\u76d2\u7528\u65bc\u5b9a\u91cf\u8840\u6e05\u6a23\u672c\u4e2d\u7684\u4e2d\u548cSARS-CoV-2\u6297\u9ad4\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u95dc\u9375\u767c\u73fe\u914d\u50998\u901a\u9053\u79fb\u6db2\u7ba1\u548c\u71b1\u632f\u76ea\u5100\u7684 OT-2 \u79fb\u6db2\u5de5\u4f5c\u7ad9\u5177\u5099\u9ad8\u7cbe\u5ea6\u81ea\u52d5\u5316\u5b8c\u6210 ELISA\u7684\u80fd\u529b\u3002\u5728\u66f4\u9ad8\u7684\u541e\u5410\u91cf\u8a2d\u5b9a\u4e2d\uff0c\u8a72\u5354\u5b9a\u53ef\u4ee5\u7528\u6700\u5c11\u7684\u64cd\u4f5c\u6642\u9593\u8655\u7406\u591a\u905496\u500b\u6a23\u672c\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u61c9\u7528\u4ecb\u7d39\u9175\u7d20\u514d\u75ab\u5206\u6790 (EIAs) \u6216\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u6e2c\u5b9a (ELISAs) \u662f\u4e00\u7a2e\u900f\u904e \u9ad8\u5ea6\u7279\u7570\u7684\u6297\u9ad4-\u6297\u539f\u7684\u76f8\u4e92\u4f5c\u7528\u5c0d\u6db2\u9ad4\u6a23\u672c\u7684\u76ee\u6a19\u5206\u5b50(\u4f8b\u5982 \u6297\u539f)\u9032\u884c\u6aa2\u6e2c\u548c\u5b9a\u91cf\u7684\u6280\u8853\u3002\u9019\u7a2e\u6e2c\u5b9a\u901a\u5e38\u572896\u5b54\u6216384\u5b54 \u677f\u4e0a\u9032\u884c\uff0c\u6211\u5011\u628a\u6297\u539f\u56fa\u5b9a\u5728\u5b54\u677f\u4e0a\uff0c\u53ef\u4ee5\u900f\u904e\u76f4\u63a5\u5c07\u6297\u539f\u9023\u63a5 \u5230\u56fa\u9ad4\u8868\u9762\u6216\u4f7f\u7528\u9810\u5857\u5728\u56fa\u9ad4\u8868\u9762\u7684\u6355\u7372\u6297\u9ad4\u4f86\u5be6\u73fe\u3002\u900f\u904e\u9019\u500b\u904e\u7a0b\uff0c\u5b83\u80fd\u5920\u5c07\u6297\u539f\u8207\u6a23\u672c\u4e2d\u5176\u4ed6\u975e\u6a19\u9776\u5206\u5b50\u5206\u958b\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u593e\u5fc3\u6cd5 ELISA\u662f\u6b64\u6e2c\u5b9a\u65b9\u6cd5\u4e2d\u6700\u5e38\u7528\u7684\u5f62\u5f0f\u3002\u5e38\u898f\u6b65\u9a5f\u5982\u4e0b\uff1a1\u3001\u5982\u679c\u4f9b\u61c9\u5546\u672a\u63d0\u4f9b\u9810\u5857\u8a72\u6297\u9ad4\u7684\u5b54\u677f\uff0c\u6211\u5011\u5247\u9700\u8981\u5c07\u6355\u7372\u6297\u9ad4(\u5373\u8207\u76ee\u6a19\u7269\u7279\u7570\u7d50\u5408\u7684\u6297\u9ad4)\u901a\u904e\u88ab\u52d5\u5438\u9644\u7684\u65b9\u5f0f\u56fa\u5b9a\u5728\u5b54\u677f\u4e0a(\u4f8b\u5982\uff0c8\u5b54\u621612\u5b54\u689d\u7d0b\u7d44\u88dd\u5728\u820796\u5b54\u5fae\u5b54\u677f\u8b80\u53d6\u5668\u76f8\u5bb9\u7684\u6846\u67b6\u4e0a)\u3002 2.\u5c07\u5f85\u5206\u6790\u7684\u6a23\u672c\u52a0\u5165\u5857\u6709\u6297\u9ad4\u7684\u677f\u4e0a\uff0c\u4f7f\u76ee\u6a19\u6297\u539f\u88ab\u6355\u7372\u3002 3.\u5c07\u56fa\u5b9a\u7684\u76ee\u6a19\u6297\u539f\u66b4\u9732\u7d66\u8207\u9175\u7d20(\u4f8b\u5982\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\u6216HRP)\u5076\u806f\u7684\u6aa2\u6e2c\u6297\u9ad4\u3002 4.\u52a0\u5165\u9175\u7d20\u7684\u5e95\u7269\uff0c\u4ee5\u7522\u751f\u53ef\u4ee5\u6e2c\u91cf\u548c\u5b9a\u91cf\u7684\u8a0a\u865f\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u4eba\u985e\u7e96\u7dad\u9023\u63a5\u86cb\u767d(FN)EIA\u8a66\u5291\u76d2 (Takara Bio,\u7f8e\u570b\u52a0\u5229\u798f\u5c3c \u4e9e\u5dde\u8056\u8377\u897f)\u662f\u4e00\u7a2e\u9ad4\u5916\u5b9a\u91cf\u6e2c\u5b9a\u8840\u6e05\u3001\u5c3f\u6db2\u3001\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2 \u548c\u5176\u4ed6\u751f\u7269\u6db2\u4e2d\u53ef\u6eb6\u6027\u4eba\u6e90 FN \u7684\u8a66\u5291\u76d2\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u8db3\u5920\u7684\u8a66\u5291\u548c\u6750\u6599\uff0c\u53ef\u7528\u65bc\u5728 OT-2 \u5e73\u53f0\u4e0a\u9032\u884c EIA\u5354\u8b70\u7684\u8a2d\u8a08\u548c\u9a57\u8b49\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>FN \u5ee3\u6cdb\u5206\u4f48\u5728\u7d30\u80de\u8868\u9762\u3001\u7d30\u80de\u5916\u57fa\u8cea\u548c\u8840\u6f3f\u4e2d\uff0c\u53c3\u8207\u7d30\u80de\u57fa\u8cea\u9ecf \u9644\u3001\u7d30\u80de\u9077\u79fb\u3001\u7d30\u80de\u5f62\u614b\u7684\u8abf\u63a7\u7b49\u7d30\u80de\u529f\u80fd\u3002 Takara \u516c\u53f8 \u7684 FN EIA\u8a66\u5291\u76d2\u63a1\u7528\u5169\u7a2e\u6297\u4eba\u6e90 FN \u7684\u9f20\u55ae\u682a\u6297\u9ad4\uff0c\u5f62\u6210\u6297\u9ad4- \u6297\u539f-\u6297\u9ad4\u593e\u5fc3\u8907\u5408\u7269\uff1a\u6355\u7372\u6297\u9ad4\u9810\u5857\u5728\u542b8\u9023\u7ba1\uff0c\u6aa2\u6e2c\u6297\u9ad4\u8207\u904e \u6c27\u5316\u7269\u9176\u7d50\u5408\u3002\u900f\u904e\u5c07\u6b64\u8907\u5408\u7269\u66b4\u9732\u65bc\u904e\u6c27\u5316\u7269\u9176\u7684\u5e95\u7269\u4e2d\uff0c\u53ef \u4ee5\u7522\u751f\u5149\u5ea6\u8a0a\u865f\uff0c\u5176\u5438\u5149\u5ea6\u8207\u6a23\u672c\u4e2d\u76ee\u6a19\u7269(\u5373\u4eba\u6e90 FN) \u7684\u6578\u91cf\u6210\u6b63\u6bd4\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7af6\u722d\u6cd5 ELISA(Enzyme-linked Immunosorbent Assay) \u901a \u904e \u6aa2\u6e2c\u8a0a\u865f\u5e79\u64fe\u4f86\u6e2c\u91cf\u6297\u539f\u7684\u6fc3\u5ea6\u3002\u7c21\u800c\u8a00\u4e4b\uff0c\u76ee\u6a19\u6297\u539f\u8207\u9810\u5148\u5857\u5728 \u5b54\u677f\u4e0a\u7684\u53c3\u7167\u7269(\u6297\u539f)\u7af6\u722d\u8207\u9175\u7d20\u5076\u806f\u6297\u9ad4\u7d50\u5408\u3002\u6a23\u672c\u4e2d\u76ee\u6a19\u6297 \u539f\u7684\u6fc3\u5ea6\u8d8a\u9ad8\uff0c\u53c3\u8003\u6297\u539f\u8207\u6297\u9ad4\u7d50\u5408\u7684\u80fd\u529b\u5c31\u8d8a\u5f31\uff0c\u7522\u751f\u7684\u8a0a\u865f \u5c31\u8d8a\u5f31\u3002 \u4e00\u4e9b\u7af6\u722d\u6cd5 ELISA \u8a66\u5291\u76d2\uff0c\u5982\u6b21\u672c\u7814\u7a76\u4e2d\u6e2c\u8a66\u7684 Tecan \u516c\u53f8\u7684 Cortisol Saliva ELISA, \u9176\u4e0d\u662f\u7531\u6297\u9ad4\u651c\u5e36\uff0c\u800c\u662f\u7531\u53c3\u7167 \u7269\u651c\u5e36\uff0c\u53c3\u7167\u7269\u8207\u76ee\u6a19\u6297\u539f\u7af6\u722d\u8207\u6297\u539f-\u6297\u9ad4\u4f5c\u7528\u3002\u6211\u5011\u5728OT-2 \u4e0a\u9032\u884c\u6e2c\u8a66\u53e6\u4e00\u500b\u7684ELISA \u8a66\u5291\u662fCell Sciences \u516c\u53f8(\u7f8e\u570b\u99ac\u85a9\u8af8\u585e\u5dde\u7d10\u4f2f\u91cc\u6e2f)\u7684SARS-CoV-2 \u66ff\u4ee3\u75c5\u6bd2\u4e2d\u548c\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c \u7528\u65bc\u6e2c\u91cfSARS-CoV-2 \u611f\u67d3\u5f8c\u6216\u5c0d\u75ab\u82d7\u63a5\u7a2e\u7522\u751f\u7684\u5faa\u74b0\u4e2d\u548c\u6297\u9ad4\u3002\u4e2d\u548c\u6297\u9ad4\u900f\u904e\u963b\u65b7\u76e4\u72c0\u75c5\u6bd2\u878d\u5408\u86cb\u767d\u7684\u53d7\u9ad4\u7d50\u5408\u57df(recep-tor-binding domain,RBD) \u8207\u8840\u7ba1\u7dca\u5f35\u7d20\u8f49\u63db\u91762 (angioten-sin converting enzyme 2,ACE2) \u7d50\u5408\uff0c\u6291\u5236SARS-CoV- 2 \u9032\u5165\u7d30\u80de\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u9810\u5148\u5857\u62b9\u7684\u75c5\u6bd2\u68d8\u86cb\u767dRBD\u677f\uff0c\u4ee5\u7af6\u722d\u6cd5ELISA \u7684\u5f62\u5f0f\uff0c\u5f15\u5165HRP- \u5076\u806f\u7684ACE2 \u8207\u4e2d\u548c\u6297\u9ad4\u7af6\u722d\u6355\u7372RBD\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u96d6\u7136 ELISA \u7684\u8a2d\u8a08\u65b9\u6cd5\u6709\u5f88\u591a\uff0c\u4f46\u842c\u8b8a\u4e0d\u96e2\u5176\u5b97\uff0c\u4ed6\u5011\u90fd\u9075\u5faa\u76f8\u4f3c\u7684\u5de5\u4f5c\u6d41\u7a0b\u3002 ELISA \u7684\u5e38\u898f\u64cd\u4f5c\u6b65\u9a5f\u5305\u62ec\u8a66\u5291\u79fb\u6db2\u3001\u5b75\u80b2\u4ee5\u53ca\u53cd\u8986\u6d17\u6ecc\uff0c\u9019\u4e9b\u6b65\u9a5f\u53ef\u4ee5\u5728 OT-2 \u4e0a\u8f15\u9b06\u5b8c\u6210\u3002\u5728\u6bcf\u500b\u8a66\u5291\u6b65\u9a5f\u4e4b\u9593\u6703\u9032\u884c\u9023\u7e8c\u7684\u6d17\u6ecc\uff0c\u4ee5\u53bb\u9664\u672a\u7d50\u5408\u7684\u5206\u5b50\u3002\u70ba\u4e86\u9632\u6b62\u5269\u9918\u7684\u6eb6\u6db2\u5e36\u5230\u4e0b\u4e00\u500b\u8a66\u5291\u6b65\u9a5f\u4e2d\uff0c\u628a\u904e\u91cf\u7684\u6db2\u9ad4\u5168\u90e8\u5438\u8d70\u975e\u5e38\u91cd\u8981\u3002 Opentrons\u76848\u901a\u9053\u79fb\u6db2\u7ba1\u53ef\u9032\u884c\u9ad8\u6548\u3001\u7cbe\u6e96\u7684\u79fb\u6db2\u8655\u7406\uff0c\u4ee5\u6eff\u8db3 ELISA \u4e2d\u8a66\u5291\u79fb\u6db2\u548c\u6d17\u6ecc\u7684\u9700\u6c42\u3002\u6b64\u5916\uff0c\u53ef\u4ee5\u900f\u904e\u6dfb\u52a0 Opentrons \u6eab\u63a7\u6a21\u7d44\u6216\u71b1\u632f\u76ea\u5100\uff0c\u4ee5\u63d0\u5347\u81ea\u52d5\u5316\u529f\u80fd\u548c\u6eff\u8db3\u5be6\u9a57\u4e2d\u7684\u6a23\u54c1\u5b75\u80b2\u74b0\u7bc0(\u4f8b\u5982\u8a2d\u5b9a\u70ba37\u2103\u4ee5\u4fc3\u9032\u6297\u539f-\u6297\u9ad4\u76f8\u4e92\u4f5c\u7528)\u6240\u9700\u7684\u6046\u6eab\u529f\u80fd\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u6750\u6599\u8207\u65b9\u6cd5\u57161-3\u986f\u793a\u4e86\u5728 Opentrons OT-2 \u4e0a\u6e2c\u8a66\u7684\u4e09\u7a2e ELISA \u7684\u793a\u610f\u5716\uff0c\u5305\u62ec\u8a66\u9a57\u6b65\u9a5f\u6982\u8ff0\u548c\u7532\u677f\u4f48\u5c40\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3051,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-104.png\" alt=\"\u5728OT-2 \u4e0a\u8fdb\u884c\u7684 Takara \u516c\u53f8\u7684\u4eba\u7c7b FN \u5939\u5fc3\u6cd5\u9176\u8054\u514d\u75ab\u5438\u9644\u68c0\u6d4b\" class=\"wp-image-3051\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":3052,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-105.png\" alt=\"\u5728OT-2\u4e0a\u8fdb\u884c\u7684 Tecan \u76ae\u8d28\u9187\u553e\u6db2\u7ade\u4e89\u6cd5 ELISA \u5b9e\u9a8c\" class=\"wp-image-3052\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":3053,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-106.png\" alt=\"\u5728OT-2\u4e0a\u8fdb\u884c\u7684 Cell Sciences SARS-CoV-2 \u66ff\u4ee3\u75c5\u6bd2\u4e2d\u548c\u5b9e\u9a8c(\u7ade\u4e89\u6cd5 ELISA)\" class=\"wp-image-3053\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u6db2\u9ad4\u8f49\u79fb\u662f\u900f\u904e8\u901a\u9053\u79fb\u6db2\u5668\u9032\u884c\u7684\u3002 ELISA \u5b54\u677f\u4f7f\u7528\u4e86\u4e00\u500b\u5e36\u6709 \u6709\u88c2\u7e2b\u5bc6\u5c01\u7684\u5c01\u819c (BioChromato, \u65e5\u672c\u85e4\u6fa4),\u9019\u6a23\u79fb\u6db2\u5668 \u7684\u5438\u982d\u53ef\u4ee5\u7a7f\u904e\u5c01\u819c\uff0c\u540c\u6642\u5728\u5b75\u80b2\u671f\u9593\u9632\u6b62\u84b8\u767c\u3002\u7136\u5f8c\uff0cELISA \u677f\u88ab\u56fa\u5b9a\u5728\u71b1\u632f\u76ea\u5100\u4e0a\uff0c\u4e26\u6309\u7167\u8aaa\u660e\u66f8\u7684\u8981\u6c42\u63d0\u4f9b\u71b1\u529b\u548c\u652a\u62cc \u4f5c\u7528(\u53c3\u8003\u88681)\u3002\u5728\u5b8c\u6210\u5be6\u9a57\u5f8c\uff0c\u9700\u8981\u7acb\u5373\u624b\u52d5\u5c07 ELISA \u5b54\u677f\u79fb\u81f3\u9176\u6a19\u5100\uff0c\u4e26\u5728450 nm \u6ce2\u9577\u8655\u6e2c\u91cf\u5438\u5149\u5ea6\u503c\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u70ba\u4e86\u6e2c\u8a66 FN EIA \u8a66\u5291\u76d2\uff0c\u9996\u5148\u5c07\u4eba\u985e\u8840\u6f3f\u4e2d\u7684 FN \u6eb6\u89e3\u5728\u53bb\u96e2\u5b50\u6c34\u4e2d\uff0c\u88fd\u5099\u51fa1 mg\/mL \u7684\u5132\u5099\u6eb6\u6db2\uff0c\u7136\u5f8c\u9032\u4e00\u6b65\u7a00\u91cb\u81f3500 ng\/mL \u4e26\u9032\u884c2\u500d\u7684\u9023\u7e8c\u7a00\u91cb\u3002\u800c\u5c0d\u65bc\u76ae\u8cea\u9187 ELISA \u6e2c\u8a66\uff0c\u5247 \u4f7f\u7528\u8a66\u5291\u76d2\u63d0\u4f9b\u7684\u5df2\u77e5\u76ae\u8cea\u9187\u6fc3\u5ea6\u7684\u6a19\u6e96\u54c1\u548c\u63a7\u5236\u6db2\u3002\u5148\u524d\u5df2 \u7d93\u6e2c\u8a66 SARS-CoV-2 \u6297\u9ad4\u967d\u6027\u6216\u9670\u6027\u7684\u8840\u6e05\u6a23\u672c\u5c07\u7528\u65bc\u75c5 \u6bd2\u4e2d\u548c ELISA \u5be6\u9a57\u3002\u6bcf\u500b\u5be6\u9a57\u5728\u4e0d\u540c\u6a23\u672c\u91cf\u4e0b\u7684\u904b\u884c\u6642\u9593\u662f\u53ef\u9810\u4f30\u7684(\u53c3\u8003\u88681)\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u5be6\u9a57\u7d50\u679c\u5c07 FN EIAs \u5728 OT-2 \u4e0a\u8655\u7406\u3002\u53ef\u4ee5\u900f\u904e\u8a55\u4f30\u6700\u7d42\u8b80\u6578\u8207\u76ee\u6a19\u5206\u5b50\u6fc3\u5ea6\u4e4b\u9593\u7684\u76f8\u95dc\u6027\u4ee5\u53ca\u4e0d\u540c\u5be6\u9a57\u4e4b\u9593\u9019\u7a2e\u76f8\u95dc\u6027\u7684\u4e00\u81f4\u6027\u4f86\u8a55\u4f30\u6a23\u672c\u8655\u7406\u54c1\u8cea\u3002\u900f\u904e\u7e6a\u88fd\u5e73\u5747\u5438\u5149\u5ea6 (n=3)\u8207 FN \u6fc3\u5ea6\u4e4b\u9593\u7684\u95dc\u4fc2\u5716\uff0c\u4e26\u8a08\u7b97\u6a19\u6e96\u504f\u5dee\uff0c\u53ef\u4ee5\u78ba\u5b9a\u7dda\u6027\u8ff4\u6b78\u7684\u64ec\u5408\u512a\u5ea6\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u7d50\u679c\u5c55\u73fe\u4e86 OT-2 \u5728\u57f7\u884c\u8a72\u8a66\u5291\u76d2\u7684\u5be6\u9a57\u4e2d\u7684\u6e96\u78ba\u6027\u548c\u7cbe\u5bc6\u5ea6(R\u5e73\u65b9&gt;0.99,CV&lt;10%) (\u57164A), \u548c\u91cd\u8907\u6027(\u57164B)\u3002\u4f7f\u7528\u76ae\u8cea\u9187\u7684\u9023\u7e8c\u7a00\u91cb\u4f86\u6e2c\u8a66OT-2\u4e0a\u7684\u7af6\u722d\u6cd5 ELISA \u3002\u7d50\u679c\u986f\u793a\u6db2\u9ad4\u8655\u7406\u7684\u4e00\u81f4\u6027(n=3,CV&lt;10%),\u5728\u534a\u5c0d\u6578\u5716\u4e0a\u5448\u73fe\u4e86\u8a66\u9a57\u8b80\u6578\u8207\u5c0d\u6578\u8f49\u63db\u5f8c\u7684\u6a23\u54c1\u6fc3\u5ea6\u4e4b\u9593\u7684\u512a\u79c0\u8ca0\u7dda\u6027\u95dc\u806f(R \u5e73\u65b9&gt;0 .99 ) ( \u57165A), \u4e14\u5be6\u9a57\u4e4b\u9593\u5177\u6709\u53ef\u91cd\u8907\u6027(\u57165C)\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3054,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-107.png\" alt=\"\u6bcf\u6b21\u6d4b\u5b9a\u7684\u70ed\u632f\u8361\u4eea\u8bbe\u7f6e\u4ee5\u53ca\u4e0d\u540c\u6837\u672c\u91cf\u7684\u9884\u8ba1\u8fd0\u884c\u65f6\u95f4\" class=\"wp-image-3054\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":3055,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-108.png\" alt=\"\u5b9e\u9a8c\u7ed3\u679c\u6570\u636e\" class=\"wp-image-3055\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u70ba\u4e86\u6aa2\u6e2c\u7af6\u722d\u6cd5 ELISA \u4e2d ACE2-RBD \u7d50\u5408\u7684\u6291\u88fd\u4f5c\u7528\uff0c\u8840\u6e05\u6a23\u672c \u5728 OT-2 \u4e0a\u9032\u884c\u4e86\u4e09\u6b21\u8655\u7406\uff0c\u5728\u9176\u6a19\u5100\u4e0a\u6e2c\u91cf\u5438\u5149\u5ea6\uff0c\u4e26\u7372\u5f97\u4e86\u8b8a\u7570\u4fc2\u6578\u3002\u7d50\u679c\u518d\u6b21\u78ba\u8a8d\u4e86 OT-2 \u6db2\u9ad4\u8655\u7406\u7684\u7cbe\u78ba\u6027 (CV&lt;10%)(\u57166)\u3002\u6839\u64da\u5ee0\u5546\u7684\u8aaa\u660e\u66f8\uff0c\u5be6\u9a57\u662f\u6709\u6548\u7684\uff0c\u56e0\u70ba\u6bcf\u500b\u63a7\u5236\u7d44 \u7684 OD450 \u503c\u90fd\u5728\u6a19\u6e96\u7bc4\u570d\u5167(\u967d\u6027\u5c0d\u7167&lt;0.3,\u9670\u6027\u5c0d\u7167&gt;0.9)\u3002\u6291\u5236\u7387\u7684\u8a08\u7b97\u516c\u5f0f\u5982\u4e0b\uff1a<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3056,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-109.png\" alt=\"\u6291\u5236\u7387\u7684\u8ba1\u7b97\u516c\u5f0f\" class=\"wp-image-3056\"><figcaption class=\"wp-element-caption\">\u900f\u904e\u5c07\u6a23\u672c\u7684\u6291\u5236\u7387\u8207\u967d\u6027\u622a\u65b7\u503c(&gt;=20%)\u6216\u9670\u6027\u622a\u65b7\u503c(&lt;20%)\u9032\u884c\u6bd4\u8f03\uff0c\u4ee5\u78ba\u5b9a\u6bcf\u500b\u6a23\u672c\u4e2d\u662f\u5426\u5b58\u5728\u4e2d\u548c\u6297\u9ad4\u3002<\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u672c\u5be6\u9a57\u6210\u529f\u6aa2\u6e2c\u5230\u5148\u524d\u88ab\u8a3a\u65b7\u70baSARS-CoV-2\u6297\u9ad4\u967d\u6027\u7684\u6a23\u672c\u4e2d\u80fd\u5920\u4e2d\u548c ACE2-RBD \u4ea4\u4e92\u4f5c\u7528\u7684\u6297\u9ad4(\u8868\u683c1)\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3057,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-110.png\" alt=\"\u5728OT-2 \u4e0a\u8fdb\u884c\u7684 Cell Sciences\u516c\u53f8\u7684SARS-CoV-2  \u66ff\u4ee3\u75c5 \u6bd2\u4e2d\u548c\u5b9e\u9a8c\" class=\"wp-image-3057\"><figcaption class=\"wp-element-caption\">\u5716 6 : \u5728OT-2 \u4e0a\u9032\u884c\u7684 Cell Sciences\u516c\u53f8\u7684SARS-CoV-2 \u66ff\u4ee3\u75c5 \u6bd2\u4e2d\u548c\u5be6\u9a57\uff0c\u4ee5\u51fa\u8272\u7684\u7cbe\u78ba\u6027\u6e2c\u91cf\u4e86\u4eba\u9ad4\u8840\u6e05\u6a23\u672c\u4e2d\u5b58\u5728\u7684\u4e2d\u548c\u6297 \u9ad4\u3002\u6839\u64da\u6bcf\u500b\u63a7\u5236\u7d44\u7684 OD450 \u503c\u90fd\u5728\u7bc4\u570d\u5167(\u967d\u6027\u5c0d\u7167&lt;0.3,\u9670\u6027\u5c0d\u7167&gt;0.9),\u8a72\u5be6\u9a57\u662f\u6709\u6548\u7684\u3002<\/figcaption><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:image {\"id\":3058,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/06\/image-111.png\" alt=\"\u4f7f\u7528\u7ecfFDA\u8ba4\u8bc1\u7684ELISA\u65b9\u6cd5\" class=\"wp-image-3058\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u5be6\u9a57\u7e3d\u7d50\u900f\u904e\u4f7f\u7528OT-2 \u9032\u884c\u7af6\u722d\u6027ELISA\u548c\u4e2d\u548c\u6297\u9ad4\u6aa2\u6e2c\uff0c\u6211\u5011\u5f97\u51fa\u7d50\u8ad6\uff1a1\u3001\u5728OT-2 \u4e0a\u9032\u884c\u7684\u7a00\u91cb\u8655\u7406\u548c\u5438\u5149\u5ea6\u6e2c\u91cf\u986f\u793a\u4e86\u9ad8\u5ea6\u4e00\u81f4\u7684\u7d50\u679c\uff0c\u8b49\u5be6\u4e86OT-2 \u6db2\u9ad4\u8655\u7406\u7684\u7cbe\u78ba\u6027\u548c\u53ef\u91cd\u8907\u6027\u3002 2.\u6839\u64da\u5ee0\u5546\u8aaa\u660e\u66f8\u7684\u8981\u6c42\uff0c\u5be6\u9a57\u7684\u63a7\u5236\u7d44\u7684 OD450 \u503c\u90fd\u5728\u6709\u6548\u7bc4\u570d\u5167\uff0c\u9a57\u8b49\u4e86\u5be6\u9a57\u7684\u6709\u6548\u6027\u3002 3.\u5229\u7528\u5be6\u9a57\u6e2c\u91cf\u7684\u6291\u5236\u7387\uff0c\u6210\u529f\u6aa2\u6e2c\u5230\u5148\u524d\u88ab\u8a3a\u65b7\u70baSARS-CoV-2 \u6297\u9ad4\u967d\u6027\u7684\u6a23\u672c\u4e2d\u5b58\u5728\u7684\u4e2d\u548c\u6297\u9ad4\u3002 4.\u8868\u683c2\u7684\u4eba\u9ad4\u8840\u6e05\u6a23\u672c\u78ba\u8a8d\u70ba\u967d\u6027\uff0c\u9032\u4e00\u6b65\u8b49\u5be6\u4e86\u5176\u5148\u524d ELISA\u6aa2\u6e2c\u7684\u7d50\u679c\u3002\u7d9c\u4e0a\u6240\u8ff0\uff0c\u8a72\u5be6\u9a57\u5728\u6aa2\u6e2c\u4e2d\u548c\u6297\u9ad4\u65b9\u9762\u8868\u73fe\u51fa\u512a\u8d8a\u7684\u7cbe\u78ba\u6027\u548c\u53ef\u9760\u6027 \uff0cOT-2 \u7684\u6a23\u54c1\u8655\u7406\u80fd\u529b\u8db3\u4ee5\u8207\u50b3\u7d71\u624b\u52d5\u65b9\u6cd5\u76f8\u5ab2\u7f8e\u3002<\/p>\n<!-- \/wp:paragraph -->","protected":false},"excerpt":{"rendered":"<p>\u4f5c\u8005Boren Lin,PhD,Kinnari Watson,PhD \u61c9\u7528\u6982\u8ff0\u9175\u7d20\u514d\u75ab\u5206\u6790 (EIA) \u6216\u9175\u7d20 [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":3052,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3050","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\u4f7f\u7528Opentrons 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