{"id":3121,"date":"2024-07-01T11:58:25","date_gmt":"2024-07-01T03:58:25","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3121"},"modified":"2024-09-02T18:28:23","modified_gmt":"2024-09-02T10:28:23","slug":"lzyyrnatqfapg","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/zh-TW\/news\/lzyyrnatqfapg\/","title":{"rendered":"\u5169\u7a2e\u7528\u65bc SARS-CoV-2 \u6aa2\u6e2c\u7684\u81ea\u52d5\u5316\u4f4e\u6210\u672c RNA \u8403\u53d6\u65b9\u6848\u7684\u8a55\u4f30"},"content":{"rendered":"\n

\u80cc\u666f<\/h3>\n\n\n\n

\u5df2\u7d93\u8a55\u4f30\u4e86\u5169\u7a2e\u7528\u65bc\u5f9e\u9f3b\u54bd\u62ed\u5b50\u4e2d\u9ad8\u901a\u91cf\u63d0\u53d6 RNA \u4ee5\u6aa2\u6e2c SARS-CoV-2 \u7684\u81ea\u52d5\u5316\u5167\u90e8\u5354\u8b70\u3002<\/p>\n\n\n\n

\u65b9\u6cd5<\/h3>\n\n\n\n

\u5728 10 \u5929\u5167\u6536\u96c6\u4e86 141 \u500b SARS-CoV-2 \u967d\u6027\u6a23\u672c\u3002\u5167\u90e8\u5354\u8b70\u57fa\u65bc\u78c1\u73e0\u63d0\u53d6\uff0c\u8a2d\u8a08\u7528\u65bcOpentrons OT-2\uff08OT-2\u5167\u90e8<\/sub>\uff09\u6db2\u9ad4\u8655\u7406\u6a5f\u5668\u4eba\u6216MagMAX TM<\/sup> Express-96 \u7cfb\u7d71\uff08MM\u5167\u90e8\uff09\u3002\u5169\u7a2e\u5354\u8b70\u5747\u8207\u4f7f\u7528 MagMAX <\/sub>TM<\/sup>\u7cfb\u7d71\uff08MM\u8a66\u5291\u76d2<\/sub>\uff09\u7684\u5546\u696d\u8a66\u5291\u76d2\u4e26\u884c\u6e2c\u8a66\u3002\u6838\u9178\u8403\u53d6\u6548\u7387\u662f\u6839\u64da SARS-CoV-2 DNA \u967d\u6027\u5c0d\u7167\u8a08\u7b97\u7684\u3002<\/p>\n\n\n\n

\u7d50\u679c<\/h3>\n\n\n\n

\u5728\u6aa2\u6e2c\u967d\u6027\u6a23\u672c\u65b9\u9762\uff0c\u5167\u90e8\u65b9\u6848\u548c\u5546\u7528\u8a66\u5291\u76d2\u4e4b\u9593\u6c92\u6709\u767c\u73fe\u986f\u8457\u5dee\u7570\u3002 MM \u8a66\u5291\u76d2<\/sub>\u6548\u7387\u6700\u9ad8\uff0c\u5118\u7ba1\u5167\u90e8 MM \u8a66\u5291\u76d2<\/sub>\u7684\u5e73\u5747 Ct \u503c\u4f4e\u65bc\u5176\u4ed6\u5169\u7a2e\u8a66\u5291\u76d2\u3002\u8207\u5546\u7528\u8a66\u5291\u76d2\u76f8\u6bd4\uff0c\u5167\u90e8\u65b9\u6848\u6bcf\u8403\u53d6 96 \u500b\u6a23\u672c\u53ef\u7bc0\u7701 350 \u6b50\u5143\u81f3 400 \u6b50\u5143\u3002<\/p>\n\n\n\n

\u7d50\u8ad6<\/h3>\n\n\n\n

\u6240\u8ff0\u5354\u8b70\u5229\u7528\u6613\u65bc\u53d6\u5f97\u7684\u8a66\u5291\u548c\u958b\u6e90\u6db2\u9ad4\u8655\u7406\u7cfb\u7d71\uff0c\u9069\u7528\u65bc\u9ad8\u901a\u91cf\u8a2d\u65bd\u4e2d\u7684 SARS-CoV-2 \u6aa2\u6e2c\u3002<\/p>\n\n\n\n

\u5f15\u7528\uff1a<\/strong> L\u00e1zaro-Perona F\u3001Rodriguez-Antol\u00edn C\u3001Alguacil-Guill\u00e9n M\u3001Guti\u00e9rrez-Arroyo A\u3001Mingorance J\u3001Garc\u00eda-Rodriguez J \u7b49\u4eba\u3002 \uff082021\uff09\u8a55\u4f30\u5169\u7a2e\u7528\u65bc SARS-CoV-2 \u6aa2\u6e2c\u7684\u81ea\u52d5\u5316\u4f4e\u6210\u672c RNA \u8403\u53d6\u65b9\u6848\u3002 PLoS ONE 16(2): e0246302\u3002 https:\/\/doi.org\/10.1371\/journal.pone.0246302<\/p>\n\n\n\n

\u7de8\u8f2f\uff1a<\/strong> AM Abd El-Aty\uff0c\u57c3\u53ca\u958b\u7f85\u5927\u5b78<\/p>\n\n\n\n

\u6536\u7a3f\u65e5\u671f\uff1a<\/strong> 2020 \u5e7411 \u67085 \u65e5\uff1b\u63a5\u53d7\u65e5\u671f\uff1a<\/strong> 2021 \u5e741 \u670815 \u65e5\uff1b\u767c\u8868\u65e5\u671f\uff1a<\/strong> 2021 \u5e742 \u670816\u65e5<\/p>\n\n\n\n

\u7248\u6b0a\u6240\u6709\uff1a<\/strong> \u00a9 2021 L\u00e1zaro-Perona \u7b49\u4eba\u3002\u9019\u662f\u4e00\u7bc7\u958b\u653e\u53d6\u7528\u7684\u6587\u7ae0\uff0c\u6839\u64da\u77e5\u8b58\u5171\u4eab\u7f72\u540d\u6388\u6b0a\u689d\u6b3e\u5206\u767c\uff0c\u5141\u8a31\u5728\u4efb\u4f55\u5a92\u9ad4\u4e2d\u4e0d\u53d7\u9650\u5236\u5730\u4f7f\u7528\u3001\u5206\u767c\u548c\u8907\u88fd\uff0c\u524d\u63d0\u662f\u8a3b\u660e\u539f\u4f5c\u8005\u548c\u4f86\u6e90\u3002<\/p>\n\n\n\n

\u8cc7\u6599\u53ef\u7528\u6027\uff1a<\/strong>\u6240\u6709\u76f8\u95dc\u8cc7\u6599\u5747\u5728\u8ad6\u6587\u53ca\u5176\u652f\u63f4\u8cc7\u8a0a\u6a94\u6848\u4e2d\u3002<\/p>\n\n\n\n

\u8cc7\u91d1\uff1a<\/strong>\u4f5c\u8005\u672a\u6536\u5230\u6b64\u9805\u5de5\u4f5c\u7684\u7279\u5b9a\u8cc7\u91d1\u8cc7\u52a9\u3002<\/p>\n\n\n\n

\u7af6\u722d\u5229\u76ca\uff1a<\/strong>\u4f5c\u8005\u5df2\u8072\u660e\u4e0d\u5b58\u5728\u7af6\u722d\u5229\u76ca\u3002<\/p>\n\n\n\n

\u4ecb\u7d39<\/h2>\n\n\n\n

SARS-CoV-2 \u75ab\u60c5\u8981\u6c42\u5927\u898f\u6a21\u4f7f\u7528 qPCR \u6aa2\u6e2c\uff0c\u4ee5\u767c\u73fe\u967d\u6027\u75c5\u4f8b\u4e26\u8ffd\u8e64\u63a5\u89f8\u8005\uff0c\u5f9e\u800c\u963b\u6b62\u793e\u5340\u50b3\u64ad\u3002\u5728 qPCR \u6aa2\u6e2c\u4e4b\u524d\uff0c\u901a\u5e38\u9700\u8981\u5c0d\u81e8\u5e8a\u6a23\u672c\u9032\u884c RNA \u8403\u53d6 [ 1-4 ]\u3002\u8003\u616e\u5230\u6bcf\u5929\u6aa2\u6e2c\u7684\u6a23\u672c\u6578\u91cf\uff0c\u5927\u591a\u6578\u6a5f\u69cb\u7121\u6cd5\u63a1\u7528\u624b\u52d5 RNA \u63d0\u53d6\u65b9\u6cd5\uff0c\u56e0\u6b64\uff0c\u81ea\u52d5\u5316\u7cfb\u7d71\u88ab\u5ee3\u6cdb\u7528\u65bc\u6b64\u4efb\u52d9 [ 5-7 ] \u3002\u81ea\u52d5\u5316\u7cfb\u7d71\u7684\u7f3a\u9ede\u662f\uff0c\u5b83\u986f\u8457\u589e\u52a0\u4e86\u6700\u7d42\u6210\u672c\uff0c\u9019\u53ef\u80fd\u6703\u963b\u7919\u67d0\u4e9b\u5730\u5340\u7684\u5927\u898f\u6a21\u6aa2\u6e2c\u3002\u6b64\u5916\uff0c\u7531\u65bc\u9700\u6c42\u589e\u52a0\uff0c\u8403\u53d6\u8a66\u5291\u7684\u5eab\u5b58\u77ed\u7f3a\u5c0e\u81f4\u8a3a\u65b7\u56b4\u91cd\u5ef6\u9072\u3002<\/p>\n\n\n\n

\u672c\u6587\u4ecb\u7d39\u4e86\u5169\u7a2e\u4f4e\u6210\u672c\u7684\u81ea\u52d5 RNA \u8403\u53d6\u65b9\u6cd5\u3002\u7b2c\u4e00\u7a2e\u65b9\u6cd5\u4f7f\u7528OT-2 \u7cfb\u7d71\uff08Opentrons\uff0c\u7d10\u7d04\u5dde\u7d10\u7d04\u5e02\uff0c\u7f8e\u570b\uff09\uff0c\u9019\u662f\u4e00\u7a2e\u80fd\u5920\u81ea\u52d5\u57f7\u884c\u81ea\u884c\u8a2d\u8a08\u65b9\u6848\u7684\u958b\u6e90\u6db2\u9ad4\u8655\u7406\u6a5f\u5668\u4eba\uff1b\u7b2c\u4e8c\u7a2e\u65b9\u6cd5\u4f7f\u7528\u5feb\u901f\u4e14\u6613\u65bc\u4f7f\u7528\u7684\u6838\u9178\u8403\u53d6\u5100MagMAX TM<\/sup> Express-96 \u7cfb\u7d71\uff08Thermo Fisher Scientific\uff0c\u9ebb\u85a9\u8af8\u585e\u5dde\u6c83\u723e\u745f\u59c6\uff0c\u7f8e\u570b\uff09\u3002\u5f8c\u8005\u53ef\u5728 30 \u5206\u9418\u5167\u63d0\u53d6\u591a\u9054 96 \u500b\u6a23\u672c\uff0c\u4f46\u9700\u8981\u4e8b\u5148\u624b\u52d5\u5206\u914d\u8a66\u5291\u3001\u78c1\u73e0\u548c 96 \u5b54\u677f\u4e2d\u7684\u6a23\u672c\uff0c\u9019\u6703\u589e\u52a0 30 \u5206\u9418\u3002\u4f5c\u70ba\u66ff\u4ee3\u65b9\u6848\uff0cOT-2\u5167\u90e8<\/sub>\u65b9\u6848\u53ef\u4ee5\u5b8c\u5168\u81ea\u52d5\u5316\u5730\u5728 104 \u5206\u9418\u5167\u8655\u7406\u591a\u9054 48 \u500b\u6a23\u672c\u3002<\/p>\n\n\n\n

\u65b9\u6cd5<\/h2>\n\n\n\n

\u6a23\u672c\u63a1\u96c6<\/h3>\n\n\n\n

\u5728\u5341\u5929\u5167\uff0c\u6536\u96c6\u4e86 141 \u4efd\u9023\u7e8c\u7684 SARS-CoV-2 \u967d\u6027\u9f3b\u54bd\u62ed\u5b50\uff0c\u9019\u4e9b\u62ed\u5b50\u5e36\u6709\u75c5\u6bd2\u904b\u8f38\u57f9\u990a\u57fa\uff08\u897f\u73ed\u7259\u5df4\u585e\u9686\u7d0d Deltalab\uff09\uff0c\u4e26\u5132\u5b58\u5728 4\u00b0C \u4e0b\u3002\u8655\u7406\u524d\uff0c\u5c07500 \u03bcL \u75c5\u6bd2\u57f9\u990a\u57fa\u548c500 \u03bcL 4M \u7570\u786b\u6c30\u9178\u80cd(GTC)\uff08\u5fb7\u570b\u5e0c\u723e\u767bQiagen\uff09\u82075 \u03bcg\/mL \u8f09\u9ad4RNA \u6df7\u5408\uff0c\u4f7f\u6a23\u54c1\u5931\u6d3b\uff0c\u7136\u5f8c\u5c07\u6a23\u54c1\u572880\u00b0C \u4e0b\u52a0\u71b12 \u5206\u9418\uff0c\u4e26\u77ed\u66ab\u6e26\u65cb\u6df7\u5408\u3002<\/p>\n\n\n\n

\u8a2d\u5099\u548c\u8a66\u5291<\/h3>\n\n\n\n

\u6838\u9178\u81ea\u52d5\u8403\u53d6\u63a1\u7528\u5169\u7a2e\u7cfb\u7d71\uff1aMagMAX TM<\/sup> Express-96 \u6df1\u5b54\u78c1\u7c89\u8655\u7406\u5668\uff08King Fisher Instrument\uff0cThermo Fisher Scientific\uff0c\u7f8e\u570b\u9ebb\u85a9\u8af8\u585e\u5dde\u6c83\u723e\u745f\u59c6\uff09\u548c\u958b\u653e\u5f0f\u7cfb\u7d71OT-2\uff08 Opentrons\uff0c\u7f8e\u570b\u7d10\u7d04\u5dde\u7d10\u7d04\uff09\uff0c\u914d\u6709GEN1 \u78c1\u6a21\u7d44\uff08Opentrons\uff0c\u7f8e\u570b\u7d10\u7d04\u5dde\u7d10\u7d04\uff09\u548c\u5167\u90e8\u5354\u8b70\u3002\u5169\u7a2e\u7cfb\u7d71\u5747\u4f7f\u7528 MagMAX\u2122 Express 96 \u677f\u548c\u6df1\u5b54\u677f\uff08Thermo Fisher Scientific\uff0c\u7f8e\u570b\u9ebb\u85a9\u8af8\u585e\u5dde\u6c83\u723e\u745f\u59c6\uff09\u3002<\/p>\n\n\n\n

\u6838\u9178\u8403\u53d6\u63a1\u7528\u4e09\u7a2e\u65b9\u6cd5\uff1a1\uff09\u6839\u64da\u88fd\u9020\u5546\u7684\u8aaa\u660e\uff0c\u4f7f\u7528MagMAX TM<\/sup>\u548c\u5546\u7528MagMAX CORE \u6838\u9178\u7d14\u5316\u8a66\u5291\u76d2(MM\u8a66\u5291\u76d2)\uff08Thermo Fisher Scientific\uff0c\u7f8e\u570b\u9ebb\u85a9\u8af8\u585e\u5dde\u6c83\u723e\u745f\u59c6\uff09\uff1b2\uff09OT-2 \u7cfb\u7d71\u63a1\u7528\u901a\u7528\u8a66\u5291\uff08OT-2\u5167\u90e8<\/sub>\uff09\uff0c\u4f8b\u5982\u4e59\u9187\uff08Emsure\u00ae\uff0cMerck KGaA\uff0c\u5fb7\u570b\u9054\u59c6\u65bd\u5854\u7279\uff09\u30012-\u4e19\u9187\uff08Emsure\u00ae\uff0cMerck KGaA\uff0c\u5fb7\u570b\u9054\u59c6\u65bd\u5854\u7279\uff09\u3001\u6d17\u812b\u7de9\u885d\u6db2\uff08Omega BIO-TEK\uff0c\u7f8e\u570b\u55ac\u6cbb\u4e9e\u5dde\u8afe\u514b\u7f85\u65af\uff09\u3001\u7121\u6838\u9178\u9176\u6c34\uff08Ambion TM<\/sup>\uff0cThermo Fisher Scientific\uff0c\u7f8e\u570b\u9ebb\u85a9\u8af8\u585e\u5dde\u6c83\u723e\u745f\u59c6\uff09\u548c\u78c1\u73e0\uff08Mag-Bind\u00ae TotalPure NGS\uff0cOmega Bio-Tek\uff0c\u7f8e\u570b\u55ac\u6cbb\u4e9e\u5dde\u8afe\u514b\u7f85\u65af\uff09\uff1b3\uff09MagMAX TM<\/sup>\u63a1\u7528\u8207\u5546\u7528\u8a66\u5291\u76d2\u76f8\u540c\u7684\u5354\u8b70\uff0c\u4f46\u4f7f\u7528OT-2 \u65b9\u6cd5\u4e2d\u7684\u8a66\u5291\uff08MM\u5167\u90e8<\/sub>\uff09\u3002\u5167\u90e8\u5354\u8b70\u662f Hui He \u7b49\u4eba\u6240\u63cf\u8ff0\u7684\u7a0b\u5e8f\u7684\u4fee\u6539\u7248[8]\u3002\u7c21\u800c\u8a00\u4e4b\uff0c\u5c07\u6ec5\u6d3b\u7684\u547c\u5438\u9053\u6a23\u672c\u4ee51:1 \u7684\u6bd4\u4f8b\u8207\u7570\u4e19\u9187\u6df7\u5408\uff0c\u81f3\u6700\u7d42\u9ad4\u7a4d\u70ba500 \u03bcl\uff08\u5167\u90e8OT-2\uff09\u548c1000 \u03bcL\uff08\u5167\u90e8<\/sub>MM\uff09\uff0c\u52a0\u516540 \u03bcL \u78c1\u73e0\u4e26\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b25 \u5206\u9418\u3002\u63a5\u4e0b\u4f86\uff0c\u7528\u78c1\u9435\u5c07\u78c1\u73e0\u62c9\u5230\u7ba1\u7684\u4e00\u5074\u4e26\u68c4\u53bb\u4e0a\u6e05\u6db2\u3002\u7136\u5f8c\u7528 500 \u03bcL \u65b0\u9bae\u88fd\u5099\u7684 70% \u4e59\u9187\u6d17\u6ecc\u78c1\u73e0\u5169\u6b21\u3002\u7b2c\u4e8c\u6b21\u6d17\u6ecc\u5f8c\uff0c\u68c4\u53bb 70% \u4e59\u9187\u4e26\u5728\u5ba4\u6eab\u4e0b\u98a8\u4e7e\u78c1\u73e0\u3002\u6700\u5f8c\uff0c\u5c07\u73e0\u5b50\u91cd\u65b0\u61f8\u6d6e\u5728 100 \u03bcL \u6d17\u812b\u7de9\u885d\u6db2\u4e2d\u4e26\u518d\u6b21\u7528\u78c1\u9435\u5206\u96e2\u4ee5\u56de\u6536\u6d17\u812b\u7684\u75c5\u6bd2 RNA\uff08\u88681\uff09\u3002<\/p>\n\n\n\n

\"\u7f29\u7565\u56fe\"<\/a><\/figure>\n\n\n\n

\u8868 1.\u6240\u8a55\u4f30\u7684\u4e09\u500b\u65b9\u6848\u4e2d\u6240\u4f7f\u7528\u7684\u6b65\u9a5f\u3001\u8a66\u5291\u548c\u9ad4\u7a4d\uff08\u03bcL\uff09\u3002<\/p>\n\n\n\n

\u5354\u8b70\u8a2d\u8a08\u8207\u9a57\u8b49<\/h3>\n\n\n\n

MM\u8a66\u5291\u76d2<\/sub>\u5354\u8b70\uff1a<\/p>\n\n\n\n

    \n
  1. \u6e96\u5099\u5169\u500b\u6df1\u5b54\u677f\uff0c\u4e00\u500b\u6df1\u5b54\u677f\u6bcf\u5b54\u88dd\u6709 500\u03bcL MagMAX\u2122 CORE Wash Solution 1\uff0c\u53e6\u4e00\u500b\u6df1\u5b54\u677f\u6bcf\u5b54\u88dd\u6709 500\u03bcL MagMAX\u2122 CORE Wash Solution 2\u3002\u6e96\u5099\u4e00\u500b MagMAX\u2122 Express 96 \u5fae\u91cf\u6ef4\u5b9a\u677f\uff0c\u6bcf\u500b\u5b54\u88dd 90\u03bcL MagMAX\u2122 CORE Elution Buffer\u3002<\/li>\n\n\n\n
  2. \u6bcf\u500b\u6a23\u54c1\u6e96\u5099 20\u03bcL MagMAX\u2122 CORE \u78c1\u73e0\u548c 10\u03bcL MagMAX\u2122 CORE \u86cb\u767d\u9176 K \u7684\u6df7\u5408\u7269\uff08\u73e0\u5b50\/PK \u6df7\u5408\u7269\uff09\u3002<\/li>\n\n\n\n
  3. \u70ba\u6bcf\u500b\u6a23\u54c1\u6e96\u5099 300\u03bcL MagMAX\u2122 CORE \u88c2\u89e3\u6db2\u548c 300\u03bcL MagMAX\u2122 CORE \u7d50\u5408\u6eb6\u6db2\u7684\u6df7\u5408\u7269\uff08\u88c2\u89e3\/\u7d50\u5408\u6eb6\u6db2\uff09\u3002<\/li>\n\n\n\n
  4. \u5728\u6df1\u5b54\u677f\u4e2d\u6bcf\u5b54\u5206\u914d 30\u03bcL \u73e0\u5b50\/PK \u6df7\u5408\u7269\u3001700\u03bcL \u88c2\u89e3\/\u7d50\u5408\u6eb6\u6db2\u548c 200\u03bcL \u6a23\u54c1\u3002<\/li>\n\n\n\n
  5. \u5c07\u6240\u6709\u677f\u653e\u5165\u7cfb\u7d71\u4e26\u57f7\u884c\u8173\u672c MagMAX_CORE_KF-96\u3002<\/li>\n<\/ol>\n\n\n\n

    MM\u5167\u90e8<\/sub>\u5354\u5b9a\uff1a<\/p>\n\n\n\n

      \n
    1. \u6e96\u5099\u5169\u500b\u6df1\u5b54\u677f\uff0c\u6bcf\u500b\u5b54\u52a0\u5165 500\u03bcL 70% \u4e59\u9187\u3002\u6e96\u5099\u4e00\u500b MagMAX\u2122 Express 96 \u5fae\u91cf\u6ef4\u5b9a\u677f\uff0c\u6bcf\u500b\u5b54\u52a0\u5165 90\u03bcL \u6d17\u812b\u7de9\u885d\u6db2\uff08Omega BIO-TEK\uff0c\u7f8e\u570b\u55ac\u6cbb\u4e9e\u5dde\u8afe\u514b\u7f85\u65af\uff09\u3002<\/li>\n\n\n\n
    2. \u5728\u6df1\u5b54\u677f\u4e2d\u6bcf\u5b54\u5206\u914d 40 \u03bcL \u78c1\u73e0\uff08Mag-Bind\u00ae TotalPure NGS\uff0cOmega Bio-Tek\uff0c\u7f8e\u570b\u55ac\u6cbb\u4e9e\u5dde\u8afe\u514b\u7f85\u65af\uff09\u3001500 \u03bcL \u7570\u4e19\u9187\u548c 500 \u03bcL \u6a23\u672c\u3002<\/li>\n\n\n\n
    3. \u5c07\u6240\u6709\u677f\u653e\u5165\u7cfb\u7d71\u4e26\u57f7\u884c\u8173\u672c MagMAX_CORE_KF-96\u3002<\/li>\n<\/ol>\n\n\n\n

      OT-2\u5167\u90e8<\/sub>\u5354\u5b9a\uff1a<\/p>\n\n\n\n

        \n
      1. \u5c07 40\u03bcL \u78c1\u73e0\uff08Mag-Bind\u00ae TotalPure NGS\uff0cOmega Bio-Tek\uff0c\u7f8e\u570b\u55ac\u6cbb\u4e9e\u5dde\u8afe\u514b\u7f85\u65af\uff09\u3001250\u03bcL \u7570\u4e19\u9187\u548c 250\u03bcL \u6a23\u672c\u5206\u88dd\u5230\u6df1\u5b54\u677f\u4e2d\u3002\u7528\u79fb\u6db2\u7ba1\u5439\u6253\u4e94\u6b21\u6df7\u52fb\uff0c\u5ba4\u6eab\u5b75\u80b2 5 \u5206\u9418\u3002<\/li>\n\n\n\n
      2. \u555f\u52d5GEN1\u78c1\u6027\u6a21\u7d444\u5206\u9418\u3002<\/li>\n\n\n\n
      3. \u6536\u96c6\u4e0a\u6e05\u6db2\u4e26\u68c4\u53bb\u3002<\/li>\n\n\n\n
      4. \u52a0\u5165500\u03bcL 70%\u4e59\u9187\uff0c\u6536\u96c6\u4e26\u4e1f\u68c4\u3002<\/li>\n\n\n\n
      5. \u52a0\u5165500\u03bcL 70%\u4e59\u9187\uff0c\u6536\u96c6\u4e26\u4e1f\u68c4\u3002<\/li>\n\n\n\n
      6. \u98a8\u4e7e 4 \u5206\u9418\u3002<\/li>\n\n\n\n
      7. \u95dc\u9589GEN1\u78c1\u6027\u6a21\u7d44\u4e26\u52a0\u5165100\u03bcL\u6d17\u812b\u7de9\u885d\u6db2\uff08Omega BIO-TEK\uff0c\u7f8e\u570b\u55ac\u6cbb\u4e9e\u5dde\u8afe\u514b\u7f85\u65af\uff09\u3002<\/li>\n\n\n\n
      8. 30\u79d2\u5f8c\u958b\u555fGEN1\u78c1\u6027\u6a21\u7d44\u3002<\/li>\n\n\n\n
      9. 90\u79d2\u5f8c\u6536\u96c6\u4e0a\u6e05\u6db2\u4e26\u8f49\u79fb\u81f396\u5b54\u5fae\u91cf\u6ef4\u5b9a\u677f\u3002<\/li>\n<\/ol>\n\n\n\n

        \u6a23\u54c1\u8f38\u5165\u91cf\u662f\u81ea\u52d5\u79fb\u6db2\u7cfb\u7d71\u5141\u8a31\u7684\u6700\u5927\u91cf\uff0c\u5176\u4ed6\u8a66\u5291\u7684\u91cf\u4e5f\u4e0d\u540c\uff0c\u56e0\u6b64\u4e09\u7a2e\u65b9\u6cd5\u7684\u8f38\u5165\u91cf\u4e0d\u540c<\/h3>\n\n\n\n

        OT-2\u5167\u90e8\u5354\u5b9a\u662f\u6839\u64da Opentrons \u8aaa\u660e\u7528 Python \u7de8\u5beb\u7684\u3002\u8173\u672c\u5df2\u5b58\u653e\u5728 GitHub \u5132\u5b58\u5eab\u4e2d<\/p>\n\n\n\n

        \u70ba\u4e86\u9a57\u8b49\u5167\u90e8\u6838\u9178\u8403\u53d6\u65b9\u6848\u7684\u6027\u80fd\uff0c\u4f7f\u7528DNA \u967d\u6027\u5c0d\u7167TaqMan 2019-nCoV \u5c0d\u7167\u8a66\u5291\u76d2v1\uff08\u8cfd\u9ed8\u98db\u4e16\u723e\u79d1\u6280\uff0c\u7f8e\u570b\u9ebb\u85a9\u8af8\u585e\u5dde\u6c83\u723e\u745f\u59c6\uff09\u88fd\u4f5c\u4e86\u6a19\u6e96\u3001\u4f4e\u548c\u6975\u4f4e\u75c5\u6bd2\u8f09\u91cf\u7684\u6a21\u64ec\u6a23\u672c\u3002\u5c0d\u7167\u8a66\u5291\u76d2\u7684\u6fc3\u5ea6\u70ba 1 x 10 4<\/sup>\u62f7\u8c9d\/\u03bcL\u3002\u6a19\u6e96\u75c5\u6bd2\u91cf\u6a23\u672c\u7684\u88fd\u5099\u65b9\u6cd5\u662f\u5c07 10 \u03bcL \u967d\u6027\u5c0d\u7167\u8207 490 \u03bcL \u75c5\u6bd2\u904b\u8f38\u57f9\u990a\u57fa\u548c 500 \u03bcL GTC \u6df7\u5408\u3002\u4f4e\u548c\u6975\u4f4e\u75c5\u6bd2\u91cf\u7684\u88fd\u5099\u65b9\u5f0f\u76f8\u540c\uff0c\u4f46\u4f7f\u7528\u967d\u6027\u5c0d\u7167\u7684\u5341\u500d\u9023\u7e8c\u7a00\u91cb\u6db2\u3002\u6240\u6709\u6a21\u64ec\u6a23\u672c\u5747\u88fd\u5099\u4e09\u4efd\uff0c\u4e26\u8207\u5167\u90e8<\/sub>OT-2 \u3001MM\u8a66\u5291\u76d2\u548c<\/sub>\u5167\u90e8<\/sub>MM\u4e26\u884c\u8655\u7406\uff0c\u7136\u5f8c\u4f7f\u7528TaqMan 2019- nCoV \u6aa2\u6e2c\u8a66\u5291\u76d2v1 \u9032\u884cqPCR \u6e2c\u8a66\u3002\u6a19\u6e96\u3001\u4f4e\u548c\u6975\u4f4e\u75c5\u6bd2\u91cf\u7684\u6a21\u64ec\u6a23\u672c\u4e2d\u967d\u6027\u5c0d\u7167\u7684\u6700\u7d42\u6fc3\u5ea6\u5206\u5225\u70ba1 x 10 6<\/sup>\u62f7\u8c9d\/mL\u30011 x 10 5<\/sup>\u62f7\u8c9d\/mL \u548c1 x 10 4<\/sup>\u62f7\u8c9d\/mL\u3002\u6240\u6709\u904b\u884c\u5747\u5305\u62ec\u9670\u6027\u5c0d\u7167\u3002<\/p>\n\n\n\n

        \u900f\u904e\u5c07\u6a21\u64ec\u6a23\u672c\u4e2d\u967d\u6027\u5c0d\u7167\u7684Ct \u503c(Ct ss<\/sub> ) \u8207\u76f4\u63a5\u5f9e\u5eab\u5b58\u88fd\u5099\u7684\u967d\u6027\u5c0d\u7167\u7684Ct \u503c\u9032\u884c\u6bd4\u8f03\u4f86\u8a08\u7b97\u6838\u9178\u63d0\u53d6\u6548\u7387\uff0c\u6821\u6b63\u91cf\u4ee5\u5339\u914d\u7a00\u91cb\u56e0\u5b50\u548c\u6bcf\u500b\u65b9\u6848\u4e2d\u4f7f\u7528\u7684\u521d\u59cb\u6a23\u672c\u91cf(Ct pc<\/sub> ) (R = 2 -\u0394Ct<\/sup> = 2 -(Ctss-Ctpc)<\/sup> )\u3002<\/p>\n\n\n\n

        \u5b9a\u91cfPCR<\/h3>\n\n\n\n

        \u4f7f\u7528\u91dd\u5c0dorf1ab<\/em>\u3001\u523a\u7a81(S)\u3001\u6838\u8863\u6bbc(N) \u548c\u4eba\u985eRNaseP \u57fa\u56e0\u7684TaqMan 2019-nCoV \u6aa2\u6e2c\u8a66\u5291\u76d2v1 \u4ee5\u53ca\u4f5c\u70ba\u967d\u6027\u5c0d\u7167\u7684TaqMan 2019-nCoV \u5c0d\u7167\u8a66\u5291\u76d2v1\uff0c\u4f9d\u7167\u88fd\u9020\u5546\u5efa\u8b70\u7684qPCR \u689d\u4ef6\u5c0dSARS-CoV-2 \u75c5\u6bd2RNA \u9032\u884c\u6838\u9178\u64f4\u589e\u3002\u6240\u6709 qPCR \u6aa2\u6e2c\u5747\u5728 CFX96 Touch \u5be6\u6642 PCR \u6aa2\u6e2c\u7cfb\u7d71 (Bio-Rad\uff0c\u7f8e\u570b\u52a0\u5dde\u8d6b\u62c9\u514b\u52d2\u65af) \u4e2d\u9032\u884c\u3002\u70ba\u4e86\u6e1b\u5c11\u6aa2\u6e2c\u9593\u5dee\u7570\uff0c\u8403\u53d6\u7684\u6838\u9178\u6a23\u672c\u4f7f\u7528\u53e6\u4e00\u500b OT-2 \u6a21\u7d44\u81ea\u52d5\u5206\u914d\u5230 qPCR \u8a66\u7d19\u4e2d\u3002<\/p>\n\n\n\n

        \u7d71\u8a08\u5206\u6790<\/h3>\n\n\n\n

        \u6210\u5c0d\u6bd4\u8f03\u4e86\u9019\u4e09\u7a2e\u65b9\u6848\u3002\u4f7f\u7528 McNemar \u6aa2\u5b9a\u4f86\u6bd4\u8f03\u5b83\u5011\u5c07\u6a23\u672c\u5206\u914d\u70ba\u967d\u6027\u6216\u9670\u6027\u7684\u8868\u73fe\u3002 Ct \u503c\u4e0d\u5448\u5e38\u614b\u5206\u4f48\uff0c\u56e0\u6b64\u4f7f\u7528 Wilcoxon \u7b26\u865f\u79e9\u6aa2\u5b9a\u4f86\u6bd4\u8f03\u6bcf\u500b\u76ee\u6a19\u7684 Ct \u503c\u3002\u5c0d\u65bc\u6bcf\u500b\u76ee\u6a19\uff0c\u50c5\u8003\u616e\u900f\u904e\u9019\u4e09\u7a2e\u65b9\u6cd5\u64f4\u589e\u7684\u6a23\u672c\u3002\u4f7f\u7528 bootstrap \u65b9\u6cd5\u8a08\u7b97\u4e2d\u4f4d\u7f6e\u4fe1\u5340\u9593\u3002<\/p>\n\n\n\n

        \u6240\u6709\u7d71\u8a08\u6aa2\u5b9a\u5747\u63a1\u7528 IBM SPSS Statistics 24.0.0.0 \u8edf\u9ad4\u5957\u4ef6\uff08SPSS Inc.\uff0c\u7f8e\u570b\u4f0a\u5229\u8afe\u5dde\u829d\u52a0\u54e5\uff09\u57f7\u884c\u3002<\/p>\n\n\n\n

        \u7d50\u679c<\/h2>\n\n\n\n

        \u8403\u53d6\u65b9\u6848\u7684\u6548\u7387<\/h3>\n\n\n\n

        \u900f\u904e\u63d0\u53d6\u6a21\u64ec\u4e0d\u540c\u75c5\u6bd2\u91cf\u7684 SARS-CoV-2 \u967d\u6027\u5c0d\u7167\u88fd\u5099\u7684\u6a21\u64ec\u6a23\u672c\uff0c\u5c07\u5169\u7a2e\u5167\u90e8\u65b9\u6848\u7684\u6838\u9178\u8403\u53d6\u6548\u7387\u8207 MM\u8a66\u5291\u76d2\u65b9\u6848\u9032\u884c\u4e86\u6bd4\u8f03\u3002 MM<\/sub>\u8a66\u5291\u76d2<\/sub>\u548cOT-2\u5167\u90e8<\/sub>\u65b9\u6848\u6210\u529f\u64f4\u589e\u4e86\u6240\u6709\u5177\u6709\u6a19\u6e96\u75c5\u6bd2\u91cf\u7684\u6a21\u64ec\u6a23\u672c\u4e2d\u7684orf1ab<\/em> \u3001S \u548cN \u6a19\u9776\u3002\u5c0d\u65bc\u9019\u4e9b\u6a23\u672c\uff0c\u6240\u6709\u91cd\u8907\u548c\u57fa\u56e0\u7684\u5e73\u5747\u8403\u53d6\u6548\u7387\u70baMM\u8a66\u5291\u76d233.9%\uff08SD\uff1a13.8\uff09\uff0cOT-2<\/sub>\u5167\u90e8<\/sub>\u65b9\u684819.6%\uff08SD\uff1a2.2\uff09 \uff0cMM\u5167\u90e8\u65b9\u6848<\/sub>16.0%\uff08SD\uff1a5.03\uff09 \u3002<\/p>\n\n\n\n

        \u5728\u4f4e\u75c5\u6bd2\u91cf\u6a21\u64ec\u6a23\u672c\u4e2d\uff0cMM\u8a66\u5291\u76d2<\/sub>\u7121\u6cd5\u64f4\u589e\u6240\u6709\u6a23\u672c\u4e2d\u7684S \u9776\u6a19\uff0c\u4e5f\u7121\u6cd5\u64f4\u589e\u5176\u4e2d\u4e00\u500b\u91cd\u8907\u6a23\u672c\u4e2d\u7684N \u9776\u6a19\uff0c\u800cOT-2\u5167\u90e8<\/sub>\u548cMM\u5167\u90e8<\/sub>\u6aa2\u6e2c\u51fa\u4e86\u6240\u6709\u57fa\u56e0\u3002\u6700\u5f8c\uff0cMM\u8a66\u5291\u76d2<\/sub>\u548cOT-2\u5167\u90e8<\/sub>\u65b9\u6848\u7121\u6cd5\u64f4\u589e\u6975\u4f4e\u75c5\u6bd2\u91cf\u6a23\u672c\uff0c\u9664\u4e86\u4e00\u500b\u91cd\u8907\u6a23\u672c\uff0c\u5176\u4e2dN \u57fa\u56e0\u53ef\u4ee5\u900f\u904eOT-2\u5167\u90e8<\/sub>\u65b9\u6848\u64f4\u589e\uff0c\u4f46MM\u5167\u90e8<\/sub>\u53ef\u4ee5\u6aa2\u6e2c\u5230\u6240\u6709\u6a23\u672c\u4e2d\u7684\u967d\u6027\u5c0d\u7167\uff0c\u5176\u4e2d\u4e00\u500b\u6a23\u672c\u542b\u6709\u4e09\u500b\u9776\u6a19\uff0c\u4e00\u500b\u6a23\u672c\u542b\u6709orf1ab\u548c<\/em> N\u6a19\u9776\uff0c\u6700\u5f8c\u4e00\u500b\u6a23\u672c\u542b\u6709orf1ab<\/em>\u6a19\u9776\uff08S1 \u8868\uff09\u3002<\/p>\n\n\n\n

        \u81e8\u5e8a\u547c\u5438\u9053\u6a23\u672c\u7684\u6838\u9178\u8403\u53d6<\/h3>\n\n\n\n

        \u6536\u96c6\u7684141 \u500b\u967d\u6027\u81e8\u5e8a\u6a23\u672c\u540c\u6642\u4f7f\u7528MM\u8a66\u5291\u76d2<\/sub>\u3001OT-2\u5167\u90e8<\/sub>\u65b9\u6cd5\u548cMM\u5167\u90e8<\/sub>\u65b9\u6cd5\u9032\u884c\u6838\u9178\u8403\u53d6\uff0c\u4e26\u900f\u904eqPCR\u6aa2\u6e2c\u6d17\u812b\u7684SARS-CoV-2 RNA\u3002\u8a18\u9304\u6bcf\u500b\u76ee\u6a19\u7684\u967d\u6027\/\u9670\u6027\u7d50\u679c\u548c\u64f4\u589e\u5faa\u74b0\u95be\u503c (Ct)\u3002\u6839\u64da\u88fd\u9020\u5546\u7684\u8aaa\u660e\uff0c\u7576\u4e09\u500b\u76ee\u6a19\u5340\u57df\u4e2d\u81f3\u5c11\u4e00\u500b\u64f4\u589e\u4e14 Ct<40 \u6642\uff0c\u6a23\u672c\u88ab\u8996\u70ba\u967d\u6027\u3002\u6240\u6709\u904b\u884c\u4e2d\u5305\u542b\u7684\u9670\u6027\u5c0d\u7167\u5728\u6240\u6709\u60c5\u6cc1\u4e0b\u5747\u7522\u751f\u9670\u6027 PCR \u7d50\u679c\u3002<\/p>\n\n\n\n

        141 \u500b\u6a23\u672c\u4e2d\uff0c123 \u500b\u6a23\u672c\u900f\u904e\u81f3\u5c11\u4e00\u7a2e\u8403\u53d6\u65b9\u6cd5\u6aa2\u6e2c\u51fa SARS-CoV-2 \u967d\u6027\uff0c\u800c 18 \u500b\u6a23\u672c\u900f\u904e\u4e09\u7a2e\u65b9\u6cd5\u6aa2\u6e2c\u5747\u70ba\u9670\u6027\u3002\u9019\u53ef\u80fd\u662f\u7531\u65bc\u6a23\u672c\u5728\u63a1\u96c6\u671f\u9593\u964d\u89e3\u6240\u81f4\uff0c\u56e0\u70ba\u5b83\u5011\u5df2\u5728 4\u00b0C \u4e0b\u5132\u5b58\u4e86\u7d04 48 \u5c0f\u6642 [9]\u3002\u4f9d\u65b9\u6cd5\u5206\u985e\uff0c114 \u500b\u6a23\u672c\u4f7f\u7528MM\u8a66\u5291\u76d2\u6aa2\u6e2c\u5448\u967d\u6027\uff0c111 \u500b\u6a23\u672c\u4f7f\u7528\u5167\u90e8<\/sub>OT-2 \u6aa2\u6e2c\u5448\u967d\u6027\uff0c118 \u500b\u6a23\u672c\u4f7f\u7528\u5167\u90e8<\/sub>MM \u6aa2\u6e2c\u5448\u967d\u6027\u3002\u6210\u5c0d\u6bd4\u8f03\u767c\u73fe\u5b83\u5011\u6aa2\u6e2cSARS-CoV-2 \u7684\u6027\u80fd\u6c92\u6709\u986f\u8457\u5dee\u7570\uff08MM\u8a66\u5291\u76d2<\/sub>Vs OT-2\u5167\u90e8<\/sub>\uff0cP =<\/em> 0.5465 \uff1bMM\u8a66\u5291\u76d2<\/sub>Vs MM\u5167\u90e8<\/sub>\uff0cP =<\/em> 0.3865\uff1bOT-2\u5167\u90e8<\/sub>Vs MM\u5167\u90e8<\/sub>\uff0cP =<\/em> 0.0961\uff09\u3002 18 \u500b\u6a23\u672c\u900f\u904e\u4e09\u7a2e\u65b9\u6cd5\u4e2d\u7684\u4e00\u4e9b\u65b9\u6cd5\u6aa2\u6e2c\u5448\u9670\u6027\u3002\u9019\u4e9b\u5177\u6709\u8f03\u9ad8\u7684 Ct \u503c\uff0c orf1ab<\/em>\u548c N \u6a19\u9776\u7684\u4e2d\u4f4d\u6578\u5206\u5225\u70ba 38.68 \u548c 38.19\uff08\u5728\u9019\u4e9b\u6a23\u672c\u4e2d\uff0cS \u6a19\u9776\u672a\u900f\u904e\u4efb\u4f55\u65b9\u6cd5\u64f4\u589e\uff09\u3002\u6bd4\u8f03\u6240\u6709\u65b9\u6cd5\u548c\u6a19\u9776\u7684\u914d\u5c0d\u7dda\u6027\u8ff4\u6b78\u548c\u76f8\u95dc\u6027\u5206\u6790\u986f\u793aR 2<\/sup>\u503c\u57280.85 \u548c0.95 \u4e4b\u9593\uff0cBland-Altman \u5206\u6790\u986f\u793a\u5728\u6574\u500bCt \u7bc4\u570d\u5167\u4e00\u81f4\u6027\u662f\u4e00\u81f4\u7684\uff08S1 \u5716\uff09\u3002<\/p>\n\n\n\n

        \u5728\u4f7f\u7528MM\u8a66\u5291\u76d2<\/sub>\u8403\u53d6\u7684\u6a23\u672c\u4e2d\uff0c43% \u53ef\u5075\u6e2c\u5230\u4e09\u500b\u6a19\u9776\uff08orf1ab \u3001 N \u548cS \uff09\uff0c\u5728\u4f7f\u7528<\/em>\u5167\u90e8<\/sub> OT-2 \u63d0\u53d6\u7684\u6a23\u672c\u4e2d\uff0c49%\u53ef\u6aa2\u6e2c\u5230\uff0c\u5728\u4f7f\u7528\u5167\u90e8<\/sub>MM \u63d0\u53d6\u7684\u6a23\u672c\u4e2d\uff0c49% \u53ef\u6aa2\u6e2c\u5230\uff08\u57161\uff09\u3002\u5728 34%\u300139% \u548c 30% \u7684\u6a23\u672c\u4e2d\u6aa2\u6e2c\u5230\u4e86orf1ab<\/em>\u548c N \u9776\u6a19\uff0c\u5728 19%\u300110% \u548c 17% \u7684\u6a23\u672c\u4e2d\u50c5\u64f4\u589e\u4e86 N \u9776\u6a19\u3002\u53ea\u6709\u6975\u5c11\u6578\u6a23\u672c\u56e0\u55ae\u7368\u64f4\u589eorf1ab<\/em>\u6a19\u9776\uff086\uff09\u3001orf1ab<\/em> + S\uff081\uff09\u6216N + S\uff085\uff09\u6a19\u9776\u800c\u88ab\u8996\u70ba\u967d\u6027\uff0c\u4e14\u6c92\u6709\u6a23\u672c\u4ee5S \u57fa\u56e0\u4f5c\u70ba\u552f\u4e00\u7684\u967d\u6027\u6a19\u8a18\u3002\u4e8b\u5be6\u4e0a\uff0c\u8a72\u6aa2\u6e2c\u4e2d\u7684 S \u6a19\u9776\u660e\u986f\u7f3a\u4e4f\u9748\u654f\u5ea6\uff0c\u4e14\u8207\u8a3a\u65b7\u6c7a\u7b56\u7121\u95dc\u3002\u4f7f\u7528OT-2\u5167\u90e8<\/sub>\u5354\u8b70\u6aa2\u6e2c\u5230\u5169\u500b\u6216\u4e09\u500b\u76ee\u6a19\u7684\u6a23\u672c\u4f5487%\uff0897 \u500b\u6a23\u672c\uff09\uff0c\u4f7f\u7528MM\u5167\u90e8\u5354\u8b70\u6aa2\u6e2c\u5230\u5169\u500b\u6216\u4e09\u500b\u76ee\u6a19\u7684\u6a23\u672c\u4f5482%\uff0895 \u500b\u6a23\u672c\uff09\uff0c\u4f7f\u7528MM\u8a66\u5291\u76d2<\/sub>\u5075\u6e2c\u5230\u5169\u500b\u6216\u4e09\u500b\u76ee\u6a19\u7684\u6a23\u672c\u4f5479% \uff0890 \u500b\u6a23\u672c\uff09\u3002<\/p>\n\n\n\n

        \"\u6837\u672c\u7684\u767e\u5206\u6bd4\"
        \u5716 1.\u4f7f\u7528 MM\u8a66\u5291\u76d2\u3001OT-2\u5167\u90e8\u65b9\u6cd5\u548c MM\u5167\u90e8\u65b9\u6cd5\u5c0dorf1ab\u3001S \u548c N \u6a19\u9776\u5448\u967d\u6027\u7684 SARS-CoV-2 \u6a23\u672c\u7684\u767e\u5206\u6bd4\u3002\u9577\u689d\u5716\u4e0a\u7684\u6578\u5b57\u8868\u793a\u6bcf\u500b\u76ee\u6a19\u7684\u967d\u6027\u6a23\u672c\u767e\u5206\u6bd4\u3002\u6574\u9ad4\u800c\u8a00\uff0c114 \u500b (81%) \u6a23\u672c\u4f7f\u7528 MM\u8a66\u5291\u76d2\u6aa2\u6e2c\u5448\u967d\u6027\uff0c111 \u500b (79%) \u4f7f\u7528\u5167\u90e8OT-2 \u6aa2\u6e2c\u5448\u967d\u6027\uff0c118 \u500b (84%) \u4f7f\u7528\u5167\u90e8MM \u6aa2\u6e2c\u5448\u967d\u6027\u3002<\/figcaption><\/figure>\n\n\n\n

        \u8003\u616e\u914d\u5c0d\u6a23\u672c\u6642\uff0c\u5728orf1ab ( P = 0.437)\u3001N ( P = 0.686) \u548cS ( P = 0.794) \u6a19\u9776\u7684Ct \u503c\u4e2d\uff0cMM \u8a66\u5291\u76d2\u548cOT-2 \u5167\u90e8\u65b9\u6848\u4e4b\u9593\u7684Cts \u6c92\u6709\u986f\u8457\u5dee\u7570( \u57162 )\u3002\u8207 MM \u8a66\u5291\u76d2 ( orf1ab<\/em> ; P<\/em> < 0.00001<\/em> , N<\/em> ; P<\/sub> < 0.00001<\/sub> , S ; P = 0.00148 )<\/em>\u548cOT<\/em> - 2<\/em>\u5167\u90e8\u65b9\u6848(<\/sub> orf1ab ;<\/em> P <<\/em> 0.00001, N; P =<\/em> 0.00008, S; P =<\/em> 0.00252)\u76f8\u6bd4\uff0cMM\u5167\u90e8\u65b9\u6848\u5728\u6240\u6709\u6a19\u9776\u4e2d\u7684Cts \u78ba\u5be6\u986f<\/sub>\u8457\u8f03\u4f4e\u3002 <\/em><\/p>\n\n\n\n

        \"\u7f29\u7565\u56fe\"<\/a><\/figure>\n\n\n\n

        \u57162.\u7bb1\u7dda\u5716\u986f\u793a\u4f7f\u7528MM\u8a66\u5291\u76d2<\/sub>\u3001OT-2\u5167\u90e8<\/sub>\u65b9\u6cd5\u548cMM\u5167\u90e8\u65b9\u6cd5\u7372\u5f97\u7684<\/sub>orf1ab \u3001S \u548cN \u76ee\u6a19\u7684Ct \u503c\u5206\u4f48\u3002 y \u8ef8\u986f\u793a\u64f4\u589e\u5faa\u74b0 (Ct)\u3002\u986f\u793a\u6bcf\u500b\u8cc7\u6599\u96c6\u4e0a\u7684\u8cc7\u6599\u9ede\u6578\u91cf\u3002<\/em><\/p>\n\n\n\n

        \u4f7f\u7528MM\u8a66\u5291\u76d2<\/sub>\u3001OT-2\u5167\u90e8\u8a66\u5291\u76d2<\/sub>\u548cMM\u5167\u90e8\u8a66\u5291\u76d2\u5c0d<\/sub>orf1ab<\/em>\u6a19\u9776\u7684\u4e2d\u4f4d\u64f4\u589e\u5faa\u74b0(CI:95%)\u5206\u5225\u70ba35.53 (33.82\u201336.36)\u300135.58 (34.33\u201336.21) \u53ca34.8 (33.71\u201335.29)\u3002\u5c0d\u65bcS \u57fa\u56e0\uff0c\u4e2d\u4f4d\u64f4\u589e\u5faa\u74b0(CI:95%) \u70ba30.16 (28.56\u201333.08)\u300131.32 (29.22\u201332.88) \u548c31.07 (29.36\u201332.90)\uff1b\u5c0d\u65bcN \u9776\u6a19\uff0c\u4e2d\u4f4d\u64f4\u589e\u5faa\u74b0(CI:95 %) \u70ba34.83 (33.93\u201335.97)\u300134.64 (33.42\u201335.29) \u548c 34.28 (33.52\u201335.10)\u3002<\/p>\n\n\n\n

        \u958b\u63a1\u6210\u672c\u548c\u5be6\u969b\u64cd\u4f5c\u6642\u9593<\/h3>\n\n\n\n

        \u4f7f\u7528OT-2\u5167\u90e8<\/sub>\u5354\u8b70\u8403\u53d696 \u500b\u6a23\u672c\u7684\u6838\u9178\uff0c\u7e3d\u6210\u672c\u70ba107 \u6b50\u5143\uff08\u8a66\u529137 \u6b50\u5143\u548c\u5be6\u9a57\u5ba4\u5668\u517770 \u6b50\u5143\uff09\uff0c\u5be6\u969b\u64cd\u4f5c\u6642\u9593\u70ba10 \u5206\u9418\uff0c\u8403\u53d6\u6642\u9593\u70ba3 \u500b\u534a\u5c0f\u6642\uff08\u8173\u672c\u6bcf\u6b21\u904b\u884c\u8655\u740648 \u500b\u6a23\u672c\uff0c\u56e0\u6b64\u5fc5\u9808\u5b8c\u6210\u5169\u6b21\uff09\u3002 MM\u5167\u90e8<\/sub>\u6210\u672c\u70ba66 \u6b50\u5143\uff08\u8a66\u529137 \u6b50\u5143\u548c\u5be6\u9a57\u5ba4\u5668\u517729 \u6b50\u5143\uff09\uff0cMM \u8a66\u5291\u76d2<\/sub>\u6210\u672c\u70ba472 \u6b50\u5143\uff08\u8a66\u5291443 \u6b50\u5143\u548c\u5be6\u9a57\u5ba4\u5668\u517729 \u6b50\u5143\uff09 \u3002\u5169\u7a2e\u5354\u8b70\u7684\u5be6\u969b\u64cd\u4f5c\u6642\u9593\u5747\u70ba 40 \u5206\u9418\uff0c\u63d0\u53d6\u6642\u9593\u5747\u70ba 30 \u5206\u9418\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u96d6\u713696 \u500b\u6a23\u672c\u7684OT-2 \u5354\u8b70\u6bd4MM\u5167\u90e8<\/sub>\u5354\u8b70\u8cb440 \u6b50\u5143\uff0c\u4f46\u6240\u9700\u7684\u521d\u59cb\u6295\u8cc7\u660e\u986f\u8f03\u4f4e\uff0cOT-2 \u7cfb\u7d71\uff08\u5305\u62ec\u6a21\u7d44\u548c\u79fb\u6db2\u5668\uff09\u7d0410,000 \u6b50\u5143\uff0cMagMAX TM<\/sup>\u7cfb\u7d71\u7d04\u70ba50,000 \u6b50\u5143\u3002<\/p>\n\n\n\n

        \u8a0e\u8ad6<\/h2>\n\n\n\n

        \u672c\u6587\u4ecb\u7d39\u4e86\u5169\u7a2e\u4f4e\u6210\u672c\u75c5\u6bd2 RNA \u8403\u53d6\u548c\u81e8\u5e8a\u6a23\u672c SARS-CoV-2 \u6aa2\u6e2c\u65b9\u6cd5\uff0c\u5176\u6027\u80fd\u8207\u5546\u7528\u8a66\u5291\u76d2\u76f8\u7576\u3002\u5118\u7ba1\u5167\u90e8\u65b9\u6848\u8403\u53d6 DNA \u5c0d\u7167\u7684\u6548\u7387\u4f4e\u65bc\u5546\u7528\u8a66\u5291\u76d2\uff0c\u4f46\u5728\u81e8\u5e8a\u6a23\u672c\u4e2d\uff0c\u6574\u9ad4\u9748\u654f\u5ea6\u4e26\u672a\u53d7\u5230\u5f71\u97ff\uff0c\u9019\u53ef\u80fd\u662f\u56e0\u70ba\u5167\u90e8\u65b9\u6848\u4f7f\u7528\u66f4\u5927\u7684\u6a23\u672c\u8d77\u59cb\u91cf\u4f86\u5f4c\u88dc\u8f03\u4f4e\u7684\u6548\u7387\u3002<\/p>\n\n\n\n

        \u8a2d\u5b9a OT-2 \u7cfb\u7d71\u9032\u884c RNA \u8403\u53d6\u9700\u8981\u5c0d\u6c92\u6709\u7d93\u9a57\u7684\u5718\u968a\u9032\u884c\u5bc6\u96c6\u7de8\u7a0b\uff0c\u4f46\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7d93\u6fdf\u9ad8\u6548\u7684\u66ff\u4ee3\u65b9\u6848\uff0c\u80fd\u5920\u5728\u4e00\u6b21\u904b\u884c\u4e2d\u63d0\u53d6 48 \u500b\u6a23\u672c\u3002\u9019\u9805\u5de5\u4f5c\u4e2d\u4f7f\u7528\u7684\u8173\u672c\u5df2\u4e0a\u50b3\u5230\u958b\u653e\u5b58\u53d6\u8edf\u9ad4\u5132\u5b58\u5eab GitHub\uff0c\u5728\u90a3\u88e1\u53ef\u4ee5\u627e\u5230\u8a31\u591a\u7528\u65bc\u9019\u4e9b\u548c\u985e\u4f3c\u61c9\u7528\u7a0b\u5f0f\u7684\u5354\u5b9a\u3002\u9019\u61c9\u8a72\u6709\u52a9\u65bc\u5176\u4ed6\u5de5\u4f5c\u4eba\u54e1\u907f\u514d\u6216\u6e1b\u5c11\u7a0b\u5f0f\u6b65\u9a5f\u3002\u8a72\u5354\u8b70\u5e7e\u4e4e\u4e0d\u9700\u8981\u52d5\u624b\u6642\u9593\uff0c\u4e26\u4e14\u4f7f\u7528\u5bb9\u6613\u7372\u5f97\u7684\u8a66\u5291\u3002\u6b64\u5916\uff0c\u8207\u5176\u4ed6\u63d0\u53d6\u7cfb\u7d71\u76f8\u6bd4\uff0c\u8a72\u8a2d\u5099\u6240\u9700\u7684\u6295\u8cc7\u8f03\u4f4e\uff0c\u4f7f\u5176\u9069\u7528\u65bc\u4e2d\u4f4e\u8cc7\u6e90\u8a2d\u65bd\u3002 MagMAX TM<\/sup> Express-96 \u662f\u4e00\u7a2e\u5feb\u901f\u3001\u534a\u81ea\u52d5\u8a2d\u5099\uff0c\u6bcf\u6b21\u904b\u884c\u80fd\u5920\u63d0\u53d6 96 \u500b\u6a23\u672c\u3002 MM\u8a66\u5291\u76d2<\/sub>\u63d0\u4f9b\u7528\u65bc\u6ec5\u6d3b\u3001\u88c2\u89e3\u3001\u6d17\u6ecc\u548c\u6d17\u812b\u7684\u8a66\u5291\uff0c\u53ef\u4ee5\u4ee5\u5177\u6709\u7af6\u722d\u529b\u7684\u6210\u672c\u7528\u65bc\u4e0d\u540c\u7684\u57fa\u8cea\u548c\u6a23\u54c1\u985e\u578b\u3002\u53e6\u4e00\u65b9\u9762\uff0cMM\u5167\u90e8<\/sub>\u5354\u8b70\u5229\u7528\u8a72\u7cfb\u7d71\u7684\u534a\u958b\u653e\u65b9\u6cd5\uff0c\u4ee5\u5176\u4ed6\u5316\u5b78\u54c1\u53d6\u4ee3\u5546\u696d\u89e3\u6c7a\u65b9\u6848\uff0c\u4f7f\u5176\u66f4\u5177\u6210\u672c\u6548\u76ca\u3002\u5728\u9019\u5169\u7a2e\u60c5\u6cc1\u4e0b\uff0c\u90fd\u5fc5\u9808\u624b\u52d5\u6e96\u5099\u6df1\u5b54\u677f\u3001\u7de9\u885d\u6db2\u548c\u6a23\u54c1\uff0c\u9019\u6703\u589e\u52a0\u7e3d\u63d0\u53d6\u6642\u9593\u548c\u64cd\u4f5c\u932f\u8aa4\u98a8\u96aa\u3002\u9019\u5169\u7a2e\u5167\u90e8\u5354\u8b70\u7684\u4e3b\u8981\u7f3a\u9ede\u662f\uff0c\u7576\u8655\u7406\u9ecf\u7a20\u6a23\u672c\u6642\uff0c\u9ecf\u6db2\u3001\u9ad8\u9ecf\u6027\u591a\u91a3\u3001\u767d\u8840\u7403\u3001\u7d05\u8840\u7403\u3001\u8840\u7d05\u86cb\u767d\u3001\u86cb\u767d\u9176\u548c\u542b\u6709\u5927\u91cf\u7d30\u80de\u6838\u9178\u7684\u7d30\u80de\u788e\u5c51\u6703\u963b\u7919RNA \u8403\u53d6\u6216\u6291\u5236PCR \u53cd\u61c9[ 10- 12 ] \u3002\u70ba\u4e86\u90e8\u5206\u514b\u670d\u9019\u500b\u554f\u984c\uff0c\u53ef\u4ee5\u5728\u8403\u53d6\u524d\u5c0d\u6a23\u54c1\u9032\u884c\u52a0\u71b1\u548c\u6e26\u65cb\u6216\u96e2\u5fc3\uff0c\u4f46\u9019\u6703\u589e\u52a0\u52d5\u624b\u6642\u9593\u548c\u53cd\u61c9\u6642\u9593\u3002\u7576\u4f7f\u7528\u5167\u90e8<\/sub>MM\u6642\uff0c\u6d17\u812b\u6a23\u672c\u5728\u67d0\u4e9b\u60c5\u6cc1\u4e0b\u53ef\u80fd\u542b\u6709\u5fae\u91cf\u7684\u78c1\u73e0\uff0c\u4f46\u9019\u4e0d\u6703\u5f71\u97ff RT-PCR \u53cd\u61c9\u7684\u8868\u73fe\u3002<\/p>\n\n\n\n

        \u5728\u9019\u4e09\u500b\u7cfb\u7d71\u4e2d\uff0c\u9670\u6027\u5c0d\u7167\u5728\u6240\u6709\u60c5\u6cc1\u4e0b\u5747\u70ba\u9670\u6027\uff0c\u986f\u793a\u5728\u9019\u4e9b\u7cfb\u7d71\u4e2d\u8655\u7406\u539f\u59cb\u6a23\u672c\u6642\u4e0d\u5b58\u5728\u4ea4\u53c9\u6c61\u67d3\u554f\u984c\u3002\u4e0d\u904e\uff0c\u8207\u975e\u81ea\u52d5\u5316\u7cfb\u7d71\u4e00\u6a23\uff0c\u61c9\u63a1\u53d6\u9810\u9632\u63aa\u65bd\uff0c\u5c07 PCR \u524d\u548c PCR \u5f8c\u64cd\u4f5c\u5206\u958b\u3002\u5982\u679c\u4f7f\u7528 OT-2 \u6a21\u7d44\u8a2d\u5b9a PCR \u5f8c\u53cd\u61c9\uff08\u4f8b\u5982\u5b9a\u5e8f\uff09\uff0c\u5247\u4e0d\u61c9\u4f7f\u7528\u5b83\u5f9e\u6a23\u672c\u4e2d\u63d0\u53d6\u6838\u9178\uff0c\u9664\u975e\u5fb9\u5e95\u6de8\u5316\u3002<\/p>\n\n\n\n

        \u7814\u7a76\u512a\u9ede\u548c\u5c40\u9650\u6027<\/h3>\n\n\n\n

        \u9019\u9805\u7814\u7a76\u7684\u4e3b\u8981\u512a\u52e2\u5728\u65bc\uff0c\u9019\u4e9b\u65b9\u6cd5\u90fd\u662f\u5728\u81e8\u5e8a\u5fae\u751f\u7269\u5be6\u9a57\u5ba4\u4e2d\u4ee5\u771f\u5be6\u6a23\u672c\u9032\u884c\u6e2c\u8a66\u7684\u3002\u65b9\u6cd5\u4e4b\u9593\u7684\u6bd4\u8f03\u4e26\u4e0d\u7e6b\u7d71\uff0c\u9019\u662f\u4e00\u500b\u91cd\u8981\u7684\u9650\u5236\u3002\u4e8b\u5be6\u4e0a\uff0c\u8a2d\u8a08\u7684\u76ee\u7684\u662f\u512a\u5316\u6bcf\u500b\u7cfb\u7d71\u7684\u7d50\u679c\uff0c\u56e0\u6b64\u8f38\u5165\u662f\u4e0d\u540c\u7684\u3002\u7cfb\u7d71\u5730\u63a2\u7d22\u8f38\u5165\u6a23\u672c\u91cf\u4ee5\u53ca\u5176\u4ed6\u8a66\u5291\u91cf\u5c07\u662f\u6709\u76ca\u548c\u6709\u76ca\u7684\uff0c\u4f46\u9019\u8d85\u51fa\u4e86\u9019\u9805\u7814\u7a76\u7684\u76ee\u7684\uff0c\u5373\u6bd4\u8f03\u81e8\u5e8a\u5be6\u9a57\u5ba4\u74b0\u5883\u4e2d\u7cfb\u7d71\u8207\u771f\u5be6\u6a23\u672c\u7684\u6027\u80fd\u3002<\/p>\n\n\n\n

        \u7d50\u8ad6<\/h2>\n\n\n\n

        \u7e3d\u4e4b\uff0c\u9019\u88e1\u4ecb\u7d39\u7684\u5169\u7a2e\u5167\u90e8<\/em>\u6838\u9178\u8403\u53d6\u65b9\u6cd5\u53ef\u6709\u6548\u5be6\u73fe SARS-CoV-2 \u7684\u9ad8\u901a\u91cf\u8a3a\u65b7\uff0c\u4e14\u6210\u672c\u50c5\u70ba\u5176\u4ed6\u5546\u696d\u8a66\u5291\u76d2\u7684\u4e00\u5c0f\u90e8\u5206\uff0c\u4e14\u4e0d\u640d\u5931\u9748\u654f\u5ea6\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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