{"id":3206,"date":"2024-07-09T11:00:32","date_gmt":"2024-07-09T03:00:32","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&#038;p=3206"},"modified":"2024-09-02T18:27:18","modified_gmt":"2024-09-02T10:27:18","slug":"tigaoyiyezhunquedu","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/zh-TW\/news\/tigaoyiyezhunquedu\/","title":{"rendered":"\u5982\u4f55\u63d0\u9ad8\u79fb\u6db2\u6e96\u78ba\u5ea6\uff1f"},"content":{"rendered":"<!-- wp:paragraph 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\/wp:paragraph -->\n\n<!-- wp:image {\"id\":3209,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-54.png\" alt=\"\u7cfb\u7edf\u8bef\u5dee\u548c\u968f\u673a\u8bef\u5dee\u4e4b\u95f4\u7684\u5173\u7cfb\" class=\"wp-image-3209\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u79fb\u6db2\u7cbe\u5ea6\u79fb\u6db2\u7cbe\u5ea6\u8861\u91cf\u79fb\u6db2\u5668\u4f7f\u7528\u8005\u63d0\u4f9b\u7b49\u65bc\u6216\u63a5\u8fd1\u9078\u5b9a\u9ad4\u7a4d\u7684\u6db2\u9ad4\u5206\u914d\u91cf\u7684\u80fd\u529b\u3002\u5206\u914d\u9ad4\u7a4d\u7684\u7cbe\u5ea6\u53d6\u6c7a\u65bc\u4ee5\u4e0b\u56e0\u7d20\uff1a<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:list -->\n<ul><!-- wp:list-item -->\n<li>\u8207\u79fb\u6db2\u5668\u5100\u5668\u548c\u5438\u982d\u76f8\u95dc\u7684\u8aa4\u5dee<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u6a23\u54c1\u4e2d\u7684\u6db2\u9ad4\u985e\u578b\uff08\u7269\u7406\u548c\/\u6216\u5316\u5b78\u6027\u8cea\uff09<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u4f7f\u7528\u8005\u6280\u8853<\/li>\n<!-- \/wp:list-item -->\n\n<!-- wp:list-item -->\n<li>\u73af\u5883\u5f71\u54cd\uff0c\u4f8b\u5982\u73af\u5883\u6e29\u5ea6\u548c\u6c14\u538b<br>\u4e0e\u79fb\u6db2\u5668\u4eea\u5668\u548c\u5438\u5934\u76f8\u5173\u7684\u8bef\u5dee<br>\u4e24\u79cd\u7c7b\u578b\u7684\u8bef\u5dee\u4f1a\u5f71\u54cd\u5355\u4e2a\u5206\u914d\u4f53\u79ef\u7684\u8d28\u91cf\uff1a<br>\u7cfb\u7edf\u8bef\u5dee<br>\u5728\u79fb\u6db2\u672f\u8bed\u4e2d\uff0c\u7cfb\u7edf\u8bef\u5dee (SE) \u8861\u91cf\u5206\u914d\u4f53\u79ef\u4e0e\u9884\u671f\u4f53\u79ef\u7684\u63a5\u8fd1\u7a0b\u5ea6\u3002<br>\u7cfb\u7edf\u8bef\u5dee\u6765\u81ea\u4eea\u5668\u672c\u8eab\uff08\u79fb\u6db2\u5668\uff09\uff0c\u5e76\u4e14\u5bf9\u4e8e\u6bcf\u4e2a\u5206\u914d\u4f53\u79ef\u90fd\u4fdd\u6301\u76f8\u540c\u7684\u503c\u6216\u6bd4\u4f8b\u3002\u7cfb\u7edf\u8bef\u5dee\u7684\u793a\u4f8b\u5305\u62ec\u4f7f\u7528\u672a\u6821\u51c6\u7684\u79fb\u6db2\u5668\u6216\u4f7f\u7528\u975e\u6807\u51c6\u5438\u5934\uff08\u4f8b\u5982\uff0c\u52a0\u957f\uff09\u3002\u4e00\u822c\u800c\u8a00\uff0c\u7cfb\u7edf\u8bef\u5dee\u662f\u53ef\u9884\u6d4b\u7684\uff0c\u56e0\u6b64\u53ef\u4ee5\u51cf\u5c11\uff08\u4f8b\u5982\uff0c\u4f7f\u7528\u6821\u51c6\u7684\u79fb\u6db2\u5668\uff09\u3002\u7cfb\u7edf\u8bef\u5dee\u53ef\u4ee5\u5b9a\u6027\u5730\u63cf\u8ff0\u4e3a\u201c\u771f\u5b9e\u5ea6\u201d\u3002<br>\u968f\u673a\u8bef\u5dee<br>\u968f\u673a\u8bef\u5dee (RE) \u662f\u5206\u914d\u4f53\u79ef\u7684\u53ef\u91cd\u590d\u6027\u3002\u5728\u79fb\u6db2\u672f\u8bed\u4e2d\uff0c\u968f\u673a\u8bef\u5dee\u8861\u91cf\u5728\u4fdd\u6301\u76f8\u540c\u5b9e\u9a8c\u6761\u4ef6\u4e0b\uff0c\u76f8\u540c\u6570\u91cf\u7684\u5206\u914d\u4f53\u79ef\u5f7c\u6b64\u4e4b\u95f4\u7684\u63a5\u8fd1\u7a0b\u5ea6\u3002\u987e\u540d\u601d\u4e49\uff0c\u968f\u673a\u8bef\u5dee\u662f\u4e0d\u53ef\u9884\u6d4b\u7684\uff0c\u5e76\u4e14\u5bf9\u4e8e\u6bcf\u4e2a\u5206\u914d\u4f53\u79ef\u800c\u8a00\u90fd\u4e0d\u76f8\u540c\u3002<br>\u968f\u673a\u8bef\u5dee\u7684\u4f8b\u5b50\u5305\u62ec\u6e29\u5ea6\u6ce2\u52a8\u548c\u79fb\u6db2\u6280\u672f\u3002\u968f\u673a\u8bef\u5dee\u53ef\u4ee5\u5b9a\u6027\u5730\u63cf\u8ff0\u4e3a\u201c\u7cbe\u5ea6\u201d\u3002<br>\u7cfb\u7edf\u8bef\u5dee\u548c\u968f\u673a\u8bef\u5dee\u5982\u4f55\u5f71\u54cd\u6574\u4f53\u79fb\u6db2\u7cbe\u5ea6\uff1f\u7cbe\u5ea6\u662f\u7cfb\u7edf\u8bef\u5dee\u548c\u968f\u673a\u8bef\u5dee\u7684\u7ec4\u5408\uff08\u56fe 1\uff09\u3002<\/li>\n<!-- \/wp:list-item --><\/ul>\n<!-- \/wp:list -->\n\n<!-- wp:paragraph -->\n<p>\u55ae\u6b21\u5206\u914d\u9ad4\u7a4d\u7684\u4e0d\u78ba\u5b9a\u6027\u539f\u5247\u4e0a\uff0c\u79fb\u6db2\u5668\u4f7f\u7528\u8005\u61c9\u6821\u6b63\u5df2\u77e5\u7cfb\u7d71\u8aa4\u5dee\u7684\u6e2c\u91cf\u503c\u3002\u5c0d\u65bc\u79fb\u6db2\u800c\u8a00\uff0c\u9019\u610f\u5473\u8457\u9700\u8981\u5f9e\u6bcf\u500b\u5206\u914d\u9ad4\u7a4d\u4e2d\u6e1b\u53bb\u7cfb\u7d71\u8aa4\u5dee\u3002\u6b64\u6b65\u9a5f\u4e0d\u592a\u5be6\u7528\u3002\u76f8\u53cd\uff0c\u300c\u55ae\u6b21\u5206\u914d\u9ad4\u7a4d\u7684\u4e0d\u78ba\u5b9a\u6027\u300d\u8003\u616e\u4e86\u7cfb\u7d71\u8aa4\u5dee\u548c\u96a8\u6a5f\u8aa4\u5dee\u3002\u5b83\u4f7f\u7528\u4ee5\u4e0b\u8fd1\u4f3c\u65b9\u7a0b\u5f0f\u8a08\u7b97\uff0c\u6b64\u5f8c\u7e2e\u5beb\u70ba\u5206\u914d\u9ad4\u7a4d\u7684\u4e0d\u78ba\u5b9a\u6027\uff1a\u5206\u914d\u9ad4\u7a4d\u7684\u4e0d\u78ba\u5b9a\u6027(\u03bcL) = | \u7cfb\u7d71\u8aa4\u5dee| + k * \u96a8\u6a5f\u8aa4\u5dee\u6b64\u65b9\u7a0b\u5f0f\u7528\u65bc\u4f30\u8a08\u55ae\u6b21\u5206\u914d\u9ad4\u7a4d\u7684\u4e0d\u78ba\u5b9a\u6027\uff0c\u7576\u79fb\u6db2\u5668\u4f7f\u7528\u8005\u9700\u8981\u9a57\u8b49\u5176\u79fb\u6db2\u5668\u7684\u6027\u80fd\u6642\u5f88\u6709\u7528\u3002\u5728\u6b64\u65b9\u7a0b\u5f0f\u4e2d\uff0ck \u662f\u64f4\u5c55\u56e0\u5b50\u3002\u64f4\u5c55\u56e0\u5b50\u4e5f\u7a31\u70ba\u8986\u84cb\u56e0\u5b50\uff0c\u6839\u64da\u5b9a\u7fa9\u7684\u6a19\u6e96\u504f\u5dee\u503c\u78ba\u5b9a\u5206\u914d\u9ad4\u7a4d\u7684\u7f6e\u4fe1\u5ea6\u3002\u4f8b\u5982\uff0c\u5982\u679c k = 1\uff0c\u5247\u5206\u914d\u9ad4\u7a4d\u7684\u7f6e\u4fe1\u5ea6\u70ba 68.3%\u3002\u5982\u679c k = 2\uff0c\u5247\u4fe1\u8cf4\u5ea6\u70ba 95.5%\uff0c\u5982\u679c k = 3\uff0c\u5247\u4fe1\u8cf4\u5ea6\u70ba 99.7%\u3002\u5be6\u969b\u4e0a\uff0c68% \u7684\u7f6e\u4fe1\u5ea6\u5f9e\u672a\u88ab\u63a8\u85a6\uff0c\u56e0\u70ba\u5b83\u5141\u8a31\u300c\u592a\u591a\u300d\u932f\u8aa4\u6e2c\u91cf\uff08\u5373\u5206\u914d\u91cf\u904e\u4f4e\u6216\u904e\u9ad8\uff09\u3002\u53e6\u4e00\u65b9\u9762\uff0c99% \u7684\u7f6e\u4fe1\u5ea6\u592a\u56b4\u683c\uff0c\u5e7e\u4e4e\u6c92\u6709\u5931\u6557\u7684\u9918\u5730\u3002\u5728\u79d1\u5b78\u5354\u8b70\u4e2d\uff0c\u901a\u5e38\u63a5\u53d7 95% \u7684\u7f6e\u4fe1\u5ea6\u3002\u5728\u79fb\u6db2\u4e2d\uff0c95% \u7684\u7f6e\u4fe1\u5ea6\u610f\u5473\u8457\uff0c\u5982\u679c\u79fb\u6db2\u5668\u4f7f\u7528\u8005\u5fc5\u9808\u5728\u76f8\u540c\u689d\u4ef6\u4e0b\uff08\u79fb\u6db2\u5668\u3001\u5438\u982d\u3001\u6280\u8853\u548c\u6a23\u54c1\uff09\u5206\u914d\u76f8\u540c\u9ad4\u7a4d20 \u6b21\uff0c\u524719 \u6b21\u5206\u914d\u5c07\u5728\u53ef\u63a5\u53d7\u7684\u4e0d\u78ba\u5b9a\u6027\uff08\u7f6e\u4fe1\u7bc4\u570d\uff09\u5167\uff0c\u4e14\u53ea\u6709\u4e00\u500b\u5206\u914d\u9ad4\u7a4d\u6703\u88ab\u62d2\u7d55\uff08\u5373\u5206\u914d\u91cf\u904e\u4f4e\u6216\u904e\u9ad8\uff09\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3208,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-53.png\" alt=\"\u5b9e\u9a8c\u6570\u636e\u6ce2\u52a8\u56fe\" class=\"wp-image-3208\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u89e3\u91cb\u79fb\u6db2\u4e2d\u7684\u4e0d\u78ba\u5b9a\u5ea6\u5982\u679c\u79fb\u6db2\u5668\u7684\u4e0d\u78ba\u5b9a\u5ea6\u70ba 20 \u00b1 2 \u00b5L\uff0c\u5247\u8868\u793a\u79fb\u6db2\u5668\u4f7f\u7528\u8005\u78ba\u4fe1\u5be6\u969b\u5206\u914d\u7684\u9ad4\u7a4d\u572818 \u81f3 22 \u00b5L \u4e4b\u9593\u3002\u89e3\u91cb\u79fb\u6db2\u4e0d\u78ba\u5b9a\u5ea6\u7684\u4e00\u7a2e\u65b9\u6cd5\u662f\u4f7f\u7528\u8005\u5c0d\u9810\u671f\u9ad4\u7a4d\u8207\u5206\u914d\u9ad4\u7a4d\u7684\u63a5\u8fd1\u7a0b\u5ea6\u7684\u6700\u4f73\u4f30\u8a08\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u6839\u64da\u5148\u524d\u7684\u4fe1\u606f\uff0c\u53ef\u4ee5\u78ba\u5b9a\u8986\u84cb\u56e0\u5b50\u662f\u7f6e\u4fe1\u6c34\u6e96\u7684\u51fd\u6578\u3002\u4ee5\u4e0b\u7bc4\u4f8b\u8aaa\u660e\u4e86\u7f6e\u4fe1\u6c34\u6e96\u548c\u91cd\u8907\u6b21\u6578\u5728\u4f30\u8a08\u5e73\u5747\u503c\u3001\u6a19\u6e96\u5dee\u548c\u4e0d\u78ba\u5b9a\u5ea6\u65b9\u9762\u7684\u5f71\u97ff\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph -->\n<p>\u79fb\u6db2\u7cbe\u5ea6\u7bc4\u4f8b\u4e00\u65e6\u78ba\u5b9a\u4e86\u7cfb\u7d71\u8aa4\u5dee\u548c\u96a8\u6a5f\u8aa4\u5dee\uff0c\u8a08\u7b97\u79fb\u6db2\u4e2d\u7684\u4e0d\u78ba\u5b9a\u5ea6\u5c31\u76f8\u5c0d\u7c21\u55ae\u4e14\u6613\u65bc\u7406\u89e3\u3002\u6848\u4f8b1\uff1a\u6a19\u7a31\u91cf\u7a0b\u5c0d\u79fb\u6db2\u7cbe\u5ea6\u7684\u5f71\u97ff\u5047\u8a2d\u4f7f\u7528\u8005\u60f3\u8981\u4f30\u8a08\u7d93\u904e\u6821\u6e96\u7684Rainin XLS+ L-20 \u79fb\u6db2\u5668\u7684\u4e0d\u78ba\u5b9a\u5ea6\uff0c\u8a72\u79fb\u6db2\u5668\u4f7f\u752810 \u6b21\u91cd\u8907\uff08\u5373\u91cd\u8907\u7684\u55ae\u6b21\u5206\u914d\u91cf\uff09\u8f38\u900120\u300110\u548c2 \u00b5L DI \u6c34\u3002\u9075\u5faa\u88fd\u9020\u5546\u91dd\u5c0d\u6b64\u7279\u5b9a 20 \u00b5L \u79fb\u6db2\u5668\u767c\u5e03\u7684\u898f\u683c\uff1a<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:image {\"id\":3207,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} -->\n<figure class=\"wp-block-image size-full\"><img src=\"https:\/\/opentrons.com.cn\/wp-content\/uploads\/2024\/07\/image-52.png\" alt=\"\u5b9e\u9a8c\u7ed3\u679c\u6570\u636e\" class=\"wp-image-3207\"><\/figure>\n<!-- \/wp:image -->\n\n<!-- wp:paragraph -->\n<p>\u4f7f\u7528\u4e0d\u78ba\u5b9a\u5ea6\u65b9\u7a0b\u5f0f\u4e0d\u78ba\u5b9a\u5ea6(\u03bcL) = | \u7cfb\u7d71\u8aa4\u5dee(\u03bcL) | + k* \u96a8\u6a5f\u8aa4\u5dee(\u03bcL)\u7136\u5f8c\uff1a\u5982\u679cV = 20 \u03bcL\uff0c\u5247\u4e0d\u78ba\u5b9a\u5ea6= | 0.20 (\u03bcL) | + k* 0.06 (\u03bcL)\u5982\u679cV = 10 \u03bcL\uff0c\u5247\u4e0d\u78ba\u5b9a\u5ea6= | 0.15 (\u03bcL) | + k* 0.05 (\u03bcL)\u5982\u679cV = 2 \u03bcL\uff0c\u5247\u4e0d\u78ba\u5b9a\u5ea6= | 0.15 (\u03bcL) | + k* 0.04 (\u03bcL)\u5982\u679c\u6240\u9700\u7684\u4fe1\u5fc3\u6c34\u6e96\u70ba 95%\uff0c\u5247\u5206\u914d\u9ad4\u7a4d\u6578\u70ba 10 (n = 10)\uff0c\u5247\u8986\u84cb\u7387\u56e0\u5b50 k = 2\u3002\u4e0d\u78ba\u5b9a\u5ea6(20 \u03bcL) = | 0.20 (\u03bcL) | + 2* 0.06 (\u03bcL) = 0.32 \u03bcL (1.6%)\u4e0d\u78ba\u5b9a\u5ea6(10 \u03bcL) = | 0.15 (\u03bcL) | + 2* 0.05 (\u03bcL) = 0.25 \u03bcL (2.5%)\u4e0d\u78ba\u5b9a\u6027(2 \u03bcL) = | 0.15 (\u03bcL) | + 2* 0.04 (\u03bcL) = 0.23 \u03bcL (11.5%)\u9019\u4e9b\u7d50\u679c\u8868\u660e\uff0c\u4e0d\u78ba\u5b9a\u6027\u8207\u6a19\u7a31\u5bb9\u91cf\u6709\u95dc\u3002\u7576\u6211\u5011\u5c07\u79fb\u6db2\u5668\u64cd\u4f5c\u5f97\u66f4\u63a5\u8fd1\u5176\u6700\u5927\u6a19\u7a31\u5bb9\u91cf (100% = 20 \u00b5L) \u6642\uff0c\u5373\u4f7f\u5047\u8a2d\u7f6e\u4fe1\u5ea6\u8f03\u9ad8\uff0c\u76f8\u5c0d\u4e0d\u78ba\u5b9a\u6027\u4e5f\u6703\u8b8a\u5f97\u66f4\u5c0f\u3002\u5728\u6240\u7d66\u51fa\u7684\u7bc4\u4f8b\u4e2d\uff0c\u8207\u79fb\u6db2\u5668\u572810% \u6a19\u7a31\u5bb9\u91cf\u9644\u8fd1\u64cd\u4f5c\u6642\u7684\u4e0d\u78ba\u5b9a\u6027\u76f8\u6bd4\uff0c\u7f6e\u4fe1\u5ea6\u70ba95.45% \u768420 \u00b5L\uff08100% \u6a19\u7a31\u5bb9\u91cf\uff09\u7684\u4e0d\u78ba\u5b9a\u6027\u8981\u5c0f\u4e03\u500d\u3002<\/p>\n<!-- \/wp:paragraph -->\n\n<!-- wp:paragraph 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