{"id":3361,"date":"2024-07-25T18:22:27","date_gmt":"2024-07-25T10:22:27","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3361"},"modified":"2024-09-02T18:23:37","modified_gmt":"2024-09-02T10:23:37","slug":"rna-tiqugongzuozhan-2","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/zh-TW\/news\/rna-tiqugongzuozhan-2\/","title":{"rendered":"\u8403\u53d6RNA\u7684\u6b65\u9a5f"},"content":{"rendered":"\n

\u5728\u751f\u7269\u79d1\u5b78\u7684\u6d69\u701a\u63a2\u7d22\u4e2d\uff0cRNA\u7684\u63d0\u53d6\u7121\u7591\u662f\u89e3\u9396\u751f\u547d\u5967\u79d8\u7684\u95dc\u9375\u4e00\u6b65\uff0c\u6bcf\u500b\u74b0\u7bc0\u90fd\u9700\u8981\u7cbe\u5fc3\u7684\u7b56\u5283\u8207\u57f7\u884c\u3002\u800c\u70ba\u4e86\u5f9e\u7d30\u80de\u3001\u7d44\u7e54\u6216\u5176\u4ed6\u751f\u7269\u6a23\u672c\u4e2d\u9ad8\u6548\u4e14\u7d14\u6de8\u5730\u5206\u96e2\u51fa\u5bf6\u8cb4\u7684RNA\u5206\u5b50\uff0c\u6211\u5011\u5fc5\u9808\u9075\u5faa\u4ee5\u4e0b\u4e00\u7cfb\u5217\u56b4\u8b39\u800c\u7d30\u7dfb\u7684\u64cd\u4f5c\u6d41\u7a0b\uff1a<\/p>\n\n\n\n

\"\u63d0\u53d6RNA\"<\/figure>\n\n\n\n

\u4e00\u3001\u5be6\u9a57\u6e96\u5099<\/p>\n\n\n\n

1.\u6e96\u5099\u8a66\u5291\u548c\u5668\u6750\uff1a\u6e96\u5099\u5be6\u9a57\u6240\u9700\u7684\u6240\u6709\u8a66\u5291\u548c\u5668\u6750\uff0c\u5982\u96e2\u5fc3\u7ba1\u3001\u79fb\u6db2\u7ba1\u3001\u7d30\u80de\u522e\u5200\u3001\u7814\u7f3d\u3001\u6db2\u614b\u6c2e\u3001RNA\u4fdd\u8b77\u5291\u3001Trizol\u8a66\u5291\u3001\u6c2f\u4eff\u3001\u7570\u4e19\u9187\u300175%\u4e59\u9187\u3001\u7121RNase\u7684\u6c34\u6216\u7de9\u885d\u6db2\u7b49\u3002<\/p>\n\n\n\n

2.\u5b89\u5168\u9632\u8b77\uff1a\u7a7f\u6234\u597d\u5be6\u9a57\u670d\u3001\u9632\u8b77\u773c\u93e1\u7b49\u5fc5\u8981\u7684\u9632\u8b77\u63aa\u65bd\uff0c\u78ba\u4fdd\u5be6\u9a57\u5b89\u5168\u3002<\/p>\n\n\n\n

3.\u95b1\u8b80\u5be6\u9a57\u6b65\u9a5f\uff1a\u4ed4\u7d30\u95b1\u8b80\u5be6\u9a57\u6b65\u9a5f\uff0c\u6e96\u5099\u8a18\u9304\u672c\uff0c\u4ee5\u4fbf\u8a18\u9304\u5be6\u9a57\u904e\u7a0b\u8207\u7d50\u679c\u3002<\/p>\n\n\n\n

\u4e8c\u3001\u6a23\u672c\u63a1\u96c6\u53ca\u8655\u7406<\/p>\n\n\n\n

1.\u9078\u64c7\u6a23\u54c1\uff1a\u4f9d\u5be6\u9a57\u8981\u6c42\u9078\u64c7\u5408\u9069\u7684\u6a23\u54c1\uff0c\u5982\u7d30\u80de\u3001\u7d44\u7e54\u3001\u8840\u6e05\u3001\u5c3f\u6db2\u7b49\u3002\u78ba\u4fdd\u6a23\u672c\u5177\u6709\u4ee3\u8868\u6027\uff0c\u4e26\u7acb\u5373\u6dfb\u52a0RNA\u4fdd\u8b77\u5291\u4ee5\u9632\u6b62RNA\u964d\u89e3\u3002<\/p>\n\n\n\n

2\u3001\u6a23\u672c\u4fdd\u5b58\uff1a\u5c07\u63a1\u96c6\u7684\u6a23\u672c\u5728\u4f4e\u6eab\u689d\u4ef6\u4e0b\uff08\u5982\u6db2\u614b\u6c2e\u6216-80\u00b0C\u51b0\u7bb1\uff09\u4fdd\u5b58\uff0c\u4ee5\u4fdd\u6301RNA\u7684\u5b8c\u6574\u6027\u3002<\/p>\n\n\n\n

\u4e09\u3001\u7d30\u80de\u7834\u788e<\/p>\n\n\n\n

1.\u6a5f\u68b0\u7834\u788e\uff1a\u4f7f\u7528\u6a5f\u68b0\u65b9\u6cd5\u5982\u9ad8\u901f\u96e2\u5fc3\u6a5f\u3001\u8d85\u97f3\u6ce2\u5668\u6216\u6db2\u614b\u6c2e\u51b7\u51cd\u7b49\u65b9\u6cd5\u5c07\u7d30\u80de\u7834\u788e\u3002\u5c0d\u65bc\u7d44\u7e54\u6a23\u54c1\uff0c\u53ef\u4ee5\u4f7f\u7528\u9810\u51b7\u7684\u7814\u7f3d\u5c07\u6a23\u54c1\u7814\u78e8\u6210\u7c89\u672b\u72c0\u3002<\/p>\n\n\n\n

2.\u5316\u5b78\u7834\u788e\uff1a\u5728\u7d30\u80de\u6216\u7d44\u7e54\u6a23\u672c\u4e2d\u52a0\u5165\u9069\u91cf\u7684\u7d30\u80de\u88c2\u89e3\u6db2\uff08\u5982Trizol\u8a66\u5291\uff09\uff0c\u4e26\u7528\u7d30\u80de\u522e\u5200\u53cd\u8986\u5439\u6253\u6a23\u54c1\uff0c\u76f4\u5230\u7d30\u80de\u5b8c\u5168\u88c2\u89e3\u3002<\/p>\n\n\n\n

\u56db\u3001RNA\u8403\u53d6<\/p>\n\n\n\n

1.\u5206\u5c64\uff1a\u5728\u7d30\u80de\u88c2\u89e3\u6db2\u4e2d\u52a0\u5165\u7b49\u9ad4\u7a4d\u7684\u6c2f\u4eff\uff0c\u5145\u5206\u6df7\u52fb\u5f8c\u96e2\u5fc3\u3002\u96e2\u5fc3\u5f8c\uff0c\u6a23\u672c\u6703\u5206\u6210\u4e09\u5c64\uff1a\u4e0a\u5c64\u70ba\u7121\u8272\u7684\u6c34\u76f8\uff08\u4e3b\u8981\u542b\u6709RNA\uff09\uff0c\u4e2d\u9593\u5c64\u70ba\u542b\u6709DNA\u7684\u5c64\uff0c\u5e95\u5c64\u70ba\u6709\u6a5f\u5c64\u3002<\/p>\n\n\n\n

2.\u8f49\u79fb\u6c34\u76f8\uff1a\u5c0f\u5fc3\u5730\u5c07\u4e0a\u5c64\u6c34\u76f8\u8f49\u79fb\u5230\u65b0\u7684\u96e2\u5fc3\u7ba1\u4e2d\uff0c\u907f\u514d\u5438\u5165\u4e2d\u9593\u5c64\u548c\u6709\u6a5f\u5c64\u3002<\/p>\n\n\n\n

3.\u6c89\u6fb1RNA\uff1a\u5728\u6c34\u76f8\u4e2d\u52a0\u5165\u7b49\u9ad4\u7a4d\u7684\u7570\u4e19\u9187\uff0c\u5145\u5206\u6df7\u52fb\u5f8c\u96e2\u5fc3\uff0c\u4f7fRNA\u6c89\u6fb1\u81f3\u96e2\u5fc3\u7ba1\u5e95\u90e8\u3002<\/p>\n\n\n\n

4.\u6d17\u6eccRNA\uff1a\u5c0f\u5fc3\u5012\u51fa\u4e0a\u6e05\u6db2\uff0c\u752875%\u4e59\u9187\u6d17\u6eccRNA\u6c89\u6fb1\uff0c\u4ee5\u53bb\u9664\u7570\u4e19\u9187\u548c\u5176\u4ed6\u96dc\u8cea\u3002<\/p>\n\n\n\n

5.\u4e7e\u71e5RNA\uff1a\u5c07\u96e2\u5fc3\u7ba1\u5728\u901a\u98a8\u8655\u667e\u4e7e\u6216\u7528\u5438\u6c34\u7d19\u5438\u4e7e\u6b98\u9918\u7684\u4e59\u9187\uff0c\u6ce8\u610f\u4e0d\u8981\u4f7fRNA\u5b8c\u5168\u4e7e\u71e5\uff0c\u4ee5\u514d\u96e3\u4ee5\u6eb6\u89e3\u3002<\/p>\n\n\n\n

\u4e94\u3001\u6eb6\u89e3RNA<\/p>\n\n\n\n

1.\u6eb6\u89e3RNA\uff1a\u5728\u96e2\u5fc3\u7ba1\u4e2d\u52a0\u5165\u9069\u91cf\u7684\u7121RNase\u6c34\u6216\u7de9\u885d\u6db2\uff0c\u8f15\u8f15\u5438\u6253\u4f7fRNA\u6c89\u6fb1\u6eb6\u89e3\u3002<\/p>\n\n\n\n

2.\u52a0\u71b1\u6eb6\u89e3\uff1a\u5c07\u96e2\u5fc3\u7ba1\u7f6e\u65bc70\u00b0C\u6c34\u6d74\u4e2d\u52a0\u71b1\u7247\u523b\uff0c\u4ee5\u4fbf\u5b8c\u5168\u6eb6\u89e3RNA\u3002<\/p>\n\n\n\n

\u516d\u3001RNA\u5b9a\u91cf\u548c\u4fdd\u5b58<\/p>\n\n\n\n

1.RNA\u5b9a\u91cf\uff1a\u4f7f\u7528\u7d2b\u5916\u7dda\u5438\u6536\u5149\u8b5c\u6216\u87a2\u5149\u5206\u6790\u5100\u7b49\u65b9\u6cd5\u6e2c\u5b9aRNA\u7684\u6fc3\u5ea6\u8207\u7d14\u5ea6\u3002<\/p>\n\n\n\n

2.RNA\u4fdd\u5b58\uff1a\u5c07RNA\u4fdd\u5b58\u5728-80\u00b0C\u7684\u4f4e\u6eab\u689d\u4ef6\u4e0b\uff0c\u6216\u52a0\u5165RNase\u6291\u5236\u5291\u7b49\u7269\u8cea\uff0c\u4ee5\u907f\u514dRNA\u7684\u964d\u89e3\u8207\u6c61\u67d3\u3002<\/p>\n\n\n\n

\u4e03\u3001\u6ce8\u610f\u4e8b\u9805<\/p>\n\n\n\n

1.\u9632\u6b62RNase\u6c61\u67d3\uff1aRNA\u9176\uff08RNase\uff09\u5ee3\u6cdb\u5b58\u5728\u65bc\u74b0\u5883\u4e2d\uff0c\u4e14\u80fd\u964d\u89e3RNA\u3002\u56e0\u6b64\uff0c\u5728\u8403\u53d6RNA\u7684\u6574\u500b\u904e\u7a0b\u4e2d\uff0c\u61c9\u4f7f\u7528\u7121RNase\u7684\u5851\u81a0\u88fd\u54c1\u548c\u73bb\u7483\u5668\u76bf\uff0c\u4e26\u7d93\u5e38\u66f4\u63db\u65b0\u624b\u5957\u4ee5\u9632\u6b62RNase\u6c61\u67d3\u3002<\/p>\n\n\n\n

2.\u63a7\u5236\u5be6\u9a57\u689d\u4ef6\uff1aRNA\u5728\u9e7c\u6027\u689d\u4ef6\u4e0b\u5bb9\u6613\u964d\u89e3\uff0c\u56e0\u6b64\u8403\u53d6RNA\u6642\u61c9\u5728pH\u503c\u4f4e\u65bc7\u7684\u689d\u4ef6\u4e0b\u9032\u884c\u3002\u540c\u6642\uff0c\u5be6\u9a57\u904e\u7a0b\u4e2d\u61c9\u6ce8\u610f\u4fdd\u6301\u4f4e\u6eab\u689d\u4ef6\u4ee5\u9632\u6b62RNA\u964d\u89e3\u3002<\/p>\n\n\n\n

3.\u6ce8\u610f\u64cd\u4f5c\u7d30\u7bc0\uff1a\u5728\u8f49\u79fb\u6c34\u76f8\u3001\u6c89\u6fb1RNA\u548c\u6d17\u6eccRNA\u7b49\u6b65\u9a5f\u4e2d\uff0c\u61c9\u5c0f\u5fc3\u64cd\u4f5c\u4ee5\u907f\u514d\u6d41\u5931RNA\u6216\u5f15\u5165\u96dc\u8cea\u3002<\/p>\n\n\n\n

\u672c\u6587\u50c5\u63d0\u4f9b\u8403\u53d6RNA\u904e\u7a0b\u7684\u4e00\u500b\u57fa\u672c\u64cd\u4f5c\u3002\u66f4\u5c08\u696d\u3001\u9ad8\u6548\u4e14\u7b26\u5408\u7279\u5b9a\u5be6\u9a57\u9700\u6c42\u7684RNA\u63d0\u53d6\uff0c\u5efa\u8b70\u5be6\u9a57\u64cd\u4f5c\u8005\u53c3\u8003\u6b0a\u5a01\u7684\u5c08\u696d\u66f8\u7c4d\u3001\u6700\u65b0\u7814\u7a76\u8ad6\u6587\u6216\u5b98\u65b9\u6280\u8853\u8cc7\u6599\u3002\u56e0\u70ba\u4e0d\u540c\u5be6\u9a57\u7684\u5177\u9ad4\u76ee\u6a19\u6703\u76f4\u63a5\u5c0e\u81f4\u8abf\u6574\u8a66\u5291\u7684\u4f7f\u7528\u91cf\u3001\u8403\u53d6\u6eab\u5ea6\u3001\u67d0\u4e9b\u6b65\u9a5f\u5ef6\u9577\u6216\u7e2e\u77ed\u7684\u6642\u9593\u7b49\u7684\u4e0d\u540c\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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