{"id":3508,"date":"2024-07-26T18:04:57","date_gmt":"2024-07-26T10:04:57","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3508"},"modified":"2024-09-02T18:22:35","modified_gmt":"2024-09-02T10:22:35","slug":"ngs-jianku","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/zh-TW\/news\/ngs-jianku\/","title":{"rendered":"\u4ec0\u9ebc\u662fngs\u5efa\u5eab"},"content":{"rendered":"\n

NGS\u5efa\u5eab\u662fNext-Generation Sequencing\uff08\u4e0b\u4e00\u4ee3\u5b9a\u5e8f\u6280\u8853\uff0c\u7c21\u7a31NGS\uff09\u6280\u8853\u6d41\u7a0b\u7684\u7b2c\u4e00\u6b65\uff0c\u4e5f\u662f\u81f3\u95dc\u91cd\u8981\u7684\u4e00\u74b0\u3002\u5b83\u6d89\u53ca\u5c07\u6a23\u672c\u4e2d\u7684DNA\u6216RNA\u5206\u5b50\u7d93\u904e\u4e00\u7cfb\u5217\u8655\u7406\u6b65\u9a5f\uff0c\u8f49\u5316\u70ba\u53ef\u4ee5\u900f\u904e\u9ad8\u901a\u91cf\u5b9a\u5e8f\u5100\u9032\u884c\u5b9a\u5e8f\u7684\u6587\u5eab\u7684\u904e\u7a0b\u3002\u672c\u6587\u5c07\u5c0dNGS\u5efa\u5eab\u9032\u884c\u8a73\u7d30\u89e3\u91cb\uff1a<\/p>\n\n\n\n

\"ngs\u5efa\u5e93\"<\/figure>\n\n\n\n

\u4e00\u3001NGS\u5efa\u5eab\u7684\u5b9a\u7fa9<\/p>\n\n\n\n

NGS\u5efa\u5eab\u662f\u6307\u5c07\u751f\u7269\u6a23\u672c\uff08\u5982\u8840\u6db2\u3001\u7d44\u7e54\u7b49\uff09\u4e2d\u7684DNA\u6216RNA\u63d0\u53d6\u51fa\u4f86\uff0c\u4e26\u900f\u904e\u4e00\u7cfb\u5217\u751f\u7269\u5316\u5b78\u624b\u6bb5\u5c07\u5176\u8f49\u5316\u70ba\u9069\u5408\u9ad8\u901a\u91cf\u5b9a\u5e8f\u5100\u8b80\u53d6\u7684\u6587\u5eab\u7684\u904e\u7a0b\u3002\u9019\u500b\u6587\u5eab\u5305\u542b\u4e86\u5927\u91cf\u7684DNA\u6216RNA\u7247\u6bb5\uff0c\u6bcf\u500b\u7247\u6bb5\u90fd\u5e36\u6709\u7279\u5b9a\u7684\u5e8f\u5217\u6a19\u7c64\uff0c\u4ee5\u4fbf\u5728\u5b9a\u5e8f\u904e\u7a0b\u4e2d\u88ab\u8b58\u5225\u548c\u5340\u5206\u3002<\/p>\n\n\n\n

\u4e8c\u3001NGS\u5efa\u5eab\u7684\u6b65\u9a5f<\/p>\n\n\n\n

NGS\u5efa\u5eab\u901a\u5e38\u5305\u62ec\u4ee5\u4e0b\u5e7e\u500b\u95dc\u9375\u6b65\u9a5f\uff1a<\/p>\n\n\n\n

1.\u6a23\u672c\u6e96\u5099\uff1a\u9996\u5148\uff0c\u9700\u8981\u5f9e\u751f\u7269\u6a23\u672c\u4e2d\u8403\u53d6DNA\u6216RNA\u3002\u9019\u4e00\u6b65\u9a5f\u901a\u5e38\u5305\u62ec\u6a23\u672c\u7684\u63a1\u96c6\u3001\u8655\u7406\u548c\u7d14\u5316\uff0c\u4ee5\u78ba\u4fdd\u63d0\u53d6\u51fa\u7684\u6838\u9178\u54c1\u8cea\u548c\u6578\u91cf\u7b26\u5408\u5f8c\u7e8c\u5be6\u9a57\u7684\u8981\u6c42\u3002<\/p>\n\n\n\n

2\u3001DNA\/RNA\u8403\u53d6\uff1a\u4f7f\u7528\u9069\u7576\u7684\u8403\u53d6\u65b9\u6cd5\uff08\u5982\u5316\u5b78\u6cd5\u3001\u7269\u7406\u6cd5\u7b49\uff09\u5f9e\u6a23\u672c\u4e2d\u5206\u96e2\u51faDNA\u6216RNA\u3002\u9019\u4e00\u6b65\u9a5f\u7684\u95dc\u9375\u662f\u78ba\u4fdd\u6838\u9178\u7684\u5b8c\u6574\u6027\u548c\u7d14\u5ea6\u3002<\/p>\n\n\n\n

3\u3001\u6587\u5eab\u5efa\u7f6e\uff1a<\/p>\n\n\n\n

1>\u7247\u6bb5\u5316\uff1a\u5c07DNA\u6216RNA\u6a23\u672c\u526a\u5207\u6210\u77ed\u7247\u6bb5\u3002\u9019\u53ef\u4ee5\u900f\u904e\u5316\u5b78\u526a\u5207\u3001\u9176\u5207\u6216\u8d85\u97f3\u6ce2\u526a\u5207\u7b49\u65b9\u6cd5\u5be6\u73fe\uff0c\u7247\u6bb5\u9577\u5ea6\u901a\u5e38\u5728100-1000bp\u4e4b\u9593\uff0c\u5177\u9ad4\u53d6\u6c7a\u65bc\u5efa\u5eab\u7684\u76ee\u7684\u548c\u5b9a\u5e8f\u5100\u7684\u8981\u6c42\u3002<\/p>\n\n\n\n

2>\u672b\u7aef\u4fee\u5fa9\u548c\u9023\u63a5\uff1a\u5c0dDNA\u6216RNA\u7247\u6bb5\u7684\u672b\u7aef\u9032\u884c\u4fee\u5fa9\uff0c\u4e26\u52a0\u5165\u9069\u7576\u7684\u9023\u7d50\u5668\uff08\u4e00\u7a2e\u77edDNA\u5e8f\u5217\uff09\uff0c\u5c07DNA\u7247\u6bb5\u9023\u63a5\u5230\u6587\u5eab\u3002\u9019\u4e00\u6b65\u662f\u70ba\u4e86\u78ba\u4fddDNA\u7247\u6bb5\u5728\u5f8c\u7e8c\u7684\u64f4\u589e\u548c\u5b9a\u5e8f\u904e\u7a0b\u4e2d\u80fd\u5920\u7a69\u5b9a\u5730\u5b58\u5728\u3002<\/p>\n\n\n\n

3>\u64f4\u589e\u548c\u7d14\u5316\uff1a\u900f\u904ePCR\u7b49\u65b9\u6cd5\u64f4\u589e\u6587\u5eab\u4e2d\u7684DNA\u7247\u6bb5\u6578\u91cf\uff0c\u4e26\u900f\u904e\u7d14\u5316\u6b65\u9a5f\u53bb\u9664\u96dc\u8cea\u548c\u672a\u9023\u63a5\u7684DNA\u7247\u6bb5\uff0c\u4ee5\u63d0\u9ad8\u6587\u5eab\u7684\u54c1\u8cea\u548c\u7d14\u5ea6\u3002<\/p>\n\n\n\n

4.\u6587\u5eab\u54c1\u7ba1\uff1a\u5c0d\u5efa\u7f6e\u597d\u7684\u6587\u5eab\u9032\u884c\u54c1\u8cea\u63a7\u5236\uff0c\u4ee5\u78ba\u4fdd\u5176\u7b26\u5408\u5b9a\u5e8f\u8981\u6c42\u3002\u5e38\u7528\u7684\u6587\u5eab\u54c1\u7ba1\u65b9\u6cd5\u5305\u62ec\u805a\u4e19\u70ef\u91af\u80fa\u51dd\u81a0\u96fb\u6cf3\u3001\u6bd4\u8272\u6cd5\u548c\u87a2\u5149\u5b9a\u91cf\u7b49\u3002<\/p>\n\n\n\n

\u4e09\u3001NGS\u5efa\u5eab\u7684\u61c9\u7528<\/p>\n\n\n\n

NGS\u5efa\u5eab\u6280\u8853\u5ee3\u6cdb\u61c9\u7528\u65bc\u57fa\u56e0\u7d44\u5b78\u3001\u8f49\u9304\u7d44\u5b78\u548c\u8868\u89c0\u57fa\u56e0\u7d44\u5b78\u7b49\u9818\u57df\u7684\u7814\u7a76\u3002\u900f\u904e\u6a23\u672c\u4e2d\u7684DNA\u6216RNA\u9032\u884c\u5efa\u5eab\u4e26\u9032\u884c\u9ad8\u901a\u91cf\u5b9a\u5e8f\uff0c\u53ef\u4ee5\u7372\u5f97\u5927\u91cf\u7684\u57fa\u56e0\u7d44\u3001\u8f49\u9304\u7d44\u548c\u8868\u89c0\u57fa\u56e0\u7d44\u8a0a\u606f\uff0c\u5e6b\u52a9\u6211\u5011\u6df1\u5165\u7406\u89e3\u751f\u547d\u7684\u5967\u79d8\u548c\u75be\u75c5\u7684\u767c\u751f\u6a5f\u5236\u3002<\/p>\n\n\n\n

\u4f8b\u5982\uff0c\u5728\u816b\u7624\u76f8\u95dc\u7814\u7a76\u4e2d\uff0cNGS\u5efa\u5eab\u6280\u8853\u53ef\u4ee5\u5e6b\u52a9\u91ab\u751f\u5feb\u901f\u4e14\u6e96\u78ba\u5730\u4e86\u89e3\u60a3\u8005\u816b\u7624\u7d30\u80deDNA\u7a81\u8b8a\u7684\u8a73\u7d30\u60c5\u6cc1\uff0c\u5f9e\u800c\u8f14\u52a9\u6cbb\u7642\u548c\u6307\u5c0e\u7528\u85e5\u60c5\u6cc1\u3002\u5728\u751f\u6b96\u907a\u50b3\u9818\u57df\uff0cNGS\u5efa\u5eab\u6280\u8853\u5247\u53ef\u900f\u904e\u975e\u4fb5\u5165\u6027\u7522\u524d\u8a3a\u65b7\u6280\u8853\u5be6\u73fe\u80ce\u5152\u907a\u50b3\u75be\u75c5\u7684\u7be9\u6aa2\u548c\u8a3a\u65b7\u3002<\/p>\n\n\n\n

\u56db\u3001\u7e3d\u7d50<\/p>\n\n\n\n

NGS\u5efa\u5eab\u662fNGS\u6280\u8853\u7684\u95dc\u9375\u6b65\u9a5f\u4e4b\u4e00\uff0c\u5b83\u900f\u904e\u5c07DNA\u6216RNA\u6a23\u672c\u8f49\u5316\u70ba\u9069\u5408\u9ad8\u901a\u91cf\u5b9a\u5e8f\u5100\u8b80\u53d6\u7684\u6587\u5eab\uff0c\u70ba\u5be6\u73fe\u5927\u898f\u6a21\u3001\u9ad8\u901a\u91cf\u7684\u57fa\u56e0\u7d44\u5b9a\u5e8f\u548c\u8f49\u9304\u7d44\u5b9a\u5e8f\u63d0\u4f9b\u4e86\u53ef\u80fd\u3002\u96a8\u8457NGS\u6280\u8853\u7684\u4e0d\u65b7\u767c\u5c55\u548c\u5b8c\u5584\uff0cNGS\u5efa\u5eab\u6280\u8853\u5c07\u5728\u66f4\u591a\u9818\u57df\u7684\u7814\u7a76\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

NGS\u5efa\u5eab\u662fNext-Generation Sequencing\uff08\u4e0b\u4e00\u4ee3\u5b9a\u5e8f\u6280\u8853\uff0c\u7c21\u7a31NGS\uff09\u6280\u8853\u6d41\u7a0b\u7684\u7b2c\u4e00\u6b65 […]<\/p>\n","protected":false},"author":5,"featured_media":3510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3508","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"\n\u4ec0\u4e48\u662fngs\u5efa\u5e93 - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/opentrons.com.cn\/zh-TW\/news\/ngs-jianku\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u4ec0\u4e48\u662fngs\u5efa\u5e93 - 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