{"id":3519,"date":"2024-07-27T17:28:54","date_gmt":"2024-07-27T09:28:54","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3519"},"modified":"2024-09-02T18:22:16","modified_gmt":"2024-09-02T10:22:16","slug":"ngs-gongzuozhanliucheng","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/zh-TW\/news\/ngs-gongzuozhanliucheng\/","title":{"rendered":"NGS\u5efa\u5eab\u5de5\u4f5c\u7ad9\u7684\u64cd\u4f5c\u6d41\u7a0b"},"content":{"rendered":"\n
NGS\uff08Next Generation 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Generation Sequencing\uff0c\u6b21\u4e16\u4ee3\u5b9a\u5e8f\uff09\u5efa\u5eab\u5de5\u4f5c\u7ad9\u7684\u64cd\u4f5c\u6b65\u9a5f\u901a\u5e38\u6d89\u53ca\u591a\u500b\u8907\u96dc […]<\/p>\n","protected":false},"author":5,"featured_media":3521,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3519","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"\nNGS\u5efa\u5e93\u5de5\u4f5c\u7ad9\u7684\u64cd\u4f5c\u6d41\u7a0b - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n\n\n\n\n\n\n\n\n\n\n\n\t\n\t\n\t\n\n\n\n\t\n\t\n\t\n
NGS\u5efa\u5eab\u5de5\u4f5c\u7ad9\u64cd\u4f5c\u6b65\u9a5f\u6982\u8ff0<\/p>\n\n\n\n
1\u3001\u6a23\u672c\u6e96\u5099<\/p>\n\n\n\n
1>\u53d6\u5f97\u6a23\u672c\uff1a\u5f9e\u751f\u7269\u9ad4\uff08\u5982\u7d30\u80de\u3001\u7d44\u7e54\u3001\u8840\u6db2\u7b49\uff09\u4e2d\u8403\u53d6DNA\u6216RNA\u3002<\/p>\n\n\n\n
2>\u54c1\u8cea\u63a7\u5236\uff1a\u8a55\u4f30\u6a23\u672c\u7684\u54c1\u8cea\u548c\u6578\u91cf\uff0c\u78ba\u4fdd\u6eff\u8db3\u5efa\u5eab\u8981\u6c42\u3002<\/p>\n\n\n\n
2\u3001\u6838\u9178\u7247\u6bb5\u5316<\/p>\n\n\n\n
1>DNA\u7247\u6bb5\u5316\uff1a\u5c0d\u65bcDNA\u5efa\u5eab\uff0c\u901a\u5e38\u4f7f\u7528\u7269\u7406\u65b9\u6cd5\uff08\u5982\u8d85\u97f3\u6ce2\u3001\u9175\u7d20\u5207\uff09\u5c07DNA\u6253\u6210\u4e00\u5b9a\u9577\u5ea6\u7684\u5c0f\u7247\u6bb5\uff08\u5982200-500bp\uff09\u3002<\/p>\n\n\n\n
2>RNA\u7247\u6bb5\u5316\uff1a\u5c0d\u65bcRNA\u5efa\u5eab\uff0c\u7279\u5225\u662fmRNA\u5efa\u5eab\uff0c\u9700\u8981\u5148\u9032\u884cmRNA\u7d14\u5316\uff0c\u7136\u5f8c\u518d\u9032\u884c\u7247\u6bb5\u5316\u8655\u7406\uff08\u5982\u4f7f\u7528\u91d1\u5c6c\u96e2\u5b50\u3001\u9175\u7d20\u8655\u7406\u7b49\uff09\u3002<\/p>\n\n\n\n
3.\u672b\u7aef\u4fee\u5fa9\u8207\u52a0\u5c3e<\/p>\n\n\n\n
1>DNA\u672b\u7aef\u4fee\u5fa9\uff1a\u4f7f\u7528\u672b\u7aef\u4fee\u5fa9\u9175\u7d20\u5c0d\u7247\u6bb5\u5316\u7684DNA\u9032\u884c5\u2019\u7aef\u78f7\u9178\u5316\u548c3\u2019\u7aef\u52a0A\u8655\u7406\uff0c\u4ee5\u4fbf\u65bc\u5f8c\u7e8c\u63a5\u982d\u7684\u9023\u63a5\u3002<\/p>\n\n\n\n
2>RNA\u53cd\u8f49\u9304\uff1a\u5c0d\u65bcRNA\u5efa\u5eab\uff0c\u9700\u8981\u5c07\u7247\u6bb5\u5316\u7684RNA\u53cd\u8f49\u9304\u6210cDNA\uff0c\u7136\u5f8c\u9032\u884c\u985e\u4f3cDNA\u7684\u672b\u7aef\u4fee\u5fa9\u548c\u52a0\u5c3e\u8655\u7406\u3002<\/p>\n\n\n\n
4.\u63a5\u982d\u9023\u63a5<\/p>\n\n\n\n
1>\u63a5\u982d\u8a2d\u8a08\uff1a\u4f9d\u7167\u5b9a\u5e8f\u5e73\u53f0\u7684\u8981\u6c42\u8a2d\u8a08\u5408\u9069\u7684\u63a5\u982d\u5e8f\u5217\u3002<\/p>\n\n\n\n
2>\u63a5\u982d\u9023\u63a5\uff1a\u4f7f\u7528DNA\u9023\u63a5\u9176\u5c07\u63a5\u982d\u9023\u63a5\u5230\u8655\u7406\u597d\u7684DNA\u6216cDNA\u7247\u6bb5\u7684\u5169\u7aef\u3002<\/p>\n\n\n\n
5.\u6587\u5eab\u64f4\u589e<\/p>\n\n\n\n
1>PCR\u64f4\u589e\uff1a\u900f\u904ePCR\u53cd\u61c9\u64f4\u589e\u9023\u63a5\u4e86\u63a5\u982d\u7684DNA\u6216cDNA\u7247\u6bb5\uff0c\u4ee5\u589e\u52a0\u6587\u5eab\u4e2d\u7684\u76ee\u6a19\u5e8f\u5217\u6fc3\u5ea6\u3002<\/p>\n\n\n\n
2>\u54c1\u8cea\u63a7\u5236\uff1a\u5c0d\u64f4\u589e\u5f8c\u7684\u6587\u5eab\u9032\u884c\u54c1\u8cea\u63a7\u5236\uff0c\u8a55\u4f30\u5176\u6fc3\u5ea6\u3001\u7d14\u5ea6\u548c\u7247\u6bb5\u5927\u5c0f\u5206\u4f48\u3002<\/p>\n\n\n\n
6.\u6587\u5eab\u7d14\u5316<\/p>\n\n\n\n
1>\u78c1\u73e0\u7d14\u5316\uff1a\u4f7f\u7528\u78c1\u73e0\u7b49\u65b9\u6cd5\u53bb\u9664\u6587\u5eab\u4e2d\u7684\u96dc\u8cea\uff08\u5982\u5f15\u5b50\u3001\u9e7d\u96e2\u5b50\u7b49\uff09\uff0c\u63d0\u9ad8\u6587\u5eab\u7684\u7d14\u5ea6\u3002<\/p>\n\n\n\n
2>\u5b9a\u91cf\u6aa2\u6e2c\uff1a\u4f7f\u7528qPCR\u7b49\u65b9\u6cd5\u5c0d\u7d14\u5316\u5f8c\u7684\u6587\u5eab\u9032\u884c\u5b9a\u91cf\u6aa2\u6e2c\uff0c\u4ee5\u4fbf\u65bc\u5f8c\u7e8c\u5b9a\u5e8f\u6642\u7684\u6a23\u672c\u5206\u914d\u3002<\/p>\n\n\n\n
7.\u6587\u5eab\u8cea\u6aa2\u8207\u5b58\u5132<\/p>\n\n\n\n
1>\u8cea\u6aa2\uff1a\u5c0d\u6700\u7d42\u6587\u5eab\u9032\u884c\u54c1\u8cea\u6aa2\u67e5\uff0c\u78ba\u4fdd\u5176\u7b26\u5408\u5b9a\u5e8f\u8981\u6c42\u3002<\/p>\n\n\n\n
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