{"id":3730,"date":"2024-07-31T17:21:02","date_gmt":"2024-07-31T09:21:02","guid":{"rendered":"https:\/\/opentrons.com.cn\/?post_type=knowledge2&p=3730"},"modified":"2024-09-02T18:20:56","modified_gmt":"2024-09-02T10:20:56","slug":"pcr-kuozeng","status":"publish","type":"post","link":"https:\/\/opentrons.com.cn\/zh-TW\/news\/pcr-kuozeng\/","title":{"rendered":"PCR\u64f4\u589e"},"content":{"rendered":"\n

\u805a\u5408\u9176\u934a\u5f0f\u53cd\u61c9\uff08PCR\uff09\u64f4\u589e\u662f\u4e00\u7a2e\u91cd\u8981\u7684\u5206\u5b50\u751f\u7269\u5b78\u6280\u8853\uff0c\u88ab\u5ee3\u6cdb\u61c9\u7528\u65bc\u57fa\u56e0\u7814\u7a76\u3001\u91ab\u5b78\u8a3a\u65b7\u4ee5\u53ca\u6cd5\u91ab\u5b78\u9451\u5b9a\u3002\u672c\u6587\u5c07\u8a73\u7d30\u4ecb\u7d39PCR\u64f4\u589e\u7684\u7279\u6027\u3001\u512a\u7f3a\u9ede\u3001\u539f\u7406\u53ca\u6d41\u7a0b\uff1a<\/p>\n\n\n\n

\"PCR\u6269\u589e\"<\/figure>\n\n\n\n

\u4e00\u3001PCR\u64f4\u589e\u7684\u7279\u9ede<\/p>\n\n\n\n

PCR\u64f4\u589e\u6280\u8853\u80fd\u5920\u5feb\u901f\u4e14\u6e96\u78ba\u5730\u8907\u88fd\u7279\u5b9a\u7684DNA\u5e8f\u5217\uff0c\u662f\u73fe\u4ee3\u751f\u7269\u5b78\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u5de5\u5177\u3002\u5176\u4e3b\u8981\u7279\u9ede\u5305\u62ec\u9ad8\u7279\u7570\u6027\u3001\u9ad8\u9748\u654f\u5ea6\u548c\u5feb\u901f\u6027\u3002\u900f\u904ePCR\u64f4\u589e\uff0c\u7814\u7a76\u4eba\u54e1\u5f97\u4ee5\u5728\u6578\u5c0f\u6642\u5167\u5c07\u76ee\u6a19DNA\u7247\u6bb5\u64f4\u589e\u6578\u767e\u842c\u500d\uff0c\u4f7f\u5f97\u5fae\u91cf\u7684DNA\u6a23\u672c\u4e5f\u80fd\u5145\u5206\u5206\u6790\u3002<\/p>\n\n\n\n

\u4e8c\u3001PCR\u64f4\u589e\u7684\u539f\u7406<\/p>\n\n\n\n

PCR\u64f4\u589e\u7684\u6838\u5fc3\u539f\u7406\u662f\u5229\u7528DNA\u805a\u5408\u9176\u5728\u7279\u5b9a\u689d\u4ef6\u4e0b\u5408\u6210DNA\u3002\u5176\u904e\u7a0b\u4e3b\u8981\u5305\u62ec\u4e09\u500b\u6b65\u9a5f\uff1a\u8b8a\u6027\u3001\u9000\u706b\u548c\u5ef6\u4f38\u3002<\/p>\n\n\n\n

1.\u8b8a\u6027\uff0cDNA\u96d9\u80a1\u5728\u9ad8\u6eab\u4e0b\u5206\u96e2\u6210\u55ae\u80a1\uff1b<\/p>\n\n\n\n

2\u3001\u9000\u706b\uff0c\u7279\u5b9a\u7684\u5f15\u5b50\u8207\u55ae\u80a1DNA\u7d50\u5408\uff1b<\/p>\n\n\n\n

3.\u5ef6\u4f38\uff0cDNA\u805a\u5408\u9176\u6cbf\u8457\u5f15\u5b50\u65b9\u5411\u5ef6\u4f38\u5408\u6210\u65b0\u7684DNA\u93c8\u3002<\/p>\n\n\n\n

\u9019\u4e09\u500b\u6b65\u9a5f\u53cd\u8986\u5faa\u74b0\uff0c\u6700\u7d42\u5be6\u73fe\u76ee\u6a19DNA\u5e8f\u5217\u7684\u6307\u6578\u7d1a\u64f4\u589e\u3002<\/p>\n\n\n\n

\u4e09\u3001PCR\u64f4\u589e\u7684\u6d41\u7a0b<\/p>\n\n\n\n

1.\u6a23\u672c\u6e96\u5099\uff1a\u5f9e\u751f\u7269\u6a23\u672c\u4e2d\u8403\u53d6DNA\uff0c\u4e26\u78ba\u5b9a\u5f85\u64f4\u589e\u7684\u76ee\u6a19\u5e8f\u5217\u3002<\/p>\n\n\n\n

2.\u53cd\u61c9\u9ad4\u7cfb\u6e96\u5099\uff1a\u5728PCR\u53cd\u61c9\u7ba1\u4e2d\u52a0\u5165\u6a21\u677fDNA\u3001\u5f15\u5b50\u3001dNTPs\uff08\u812b\u6c27\u6838\u82f7\u9178\u4e09\u78f7\u9178\uff09\u3001DNA\u805a\u5408\u9176\u548c\u7de9\u885d\u6db2\u7b49\u53cd\u61c9\u7d44\u5206\u3002<\/p>\n\n\n\n

3.\u8b8a\u6027\uff1a\u5c07\u53cd\u61c9\u9ad4\u7cfb\u52a0\u71b1\u81f394-98\u2103\uff0c\u4f7f\u96d9\u80a1DNA\u5206\u96e2\u6210\u55ae\u80a1\u3002<\/p>\n\n\n\n

4.\u9000\u706b\uff1a\u5c07\u6eab\u5ea6\u964d\u4f4e\u81f350-65\u2103\uff0c\u5f15\u5b50\u8207\u55ae\u80a1DNA\u7d50\u5408\u3002<\/p>\n\n\n\n

5.\u5ef6\u4f38\uff1a\u5c07\u6eab\u5ea6\u5347\u81f372\u2103\uff0cDNA\u805a\u5408\u9176\u6cbf\u5f15\u5b50\u65b9\u5411\u5408\u6210\u65b0\u7684DNA\u93c8\u3002<\/p>\n\n\n\n

6.\u5faa\u74b0\uff1a\u91cd\u8907\u4e0a\u8ff0\u8b8a\u6027\u3001\u9000\u706b\u548c\u5ef6\u4f38\u6b65\u9a5f30-40\u6b21\uff0c\u6700\u7d42\u7372\u5f97\u5927\u91cf\u76ee\u6a19DNA\u7247\u6bb5\u3002<\/p>\n\n\n\n

7.\u6aa2\u6e2c\uff1a\u4ee5\u96fb\u6cf3\u7b49\u65b9\u6cd5\u6aa2\u6e2cPCR\u64f4\u589e\u7522\u7269\uff0c\u78ba\u8a8d\u64f4\u589e\u7d50\u679c\u3002<\/p>\n\n\n\n

\u56db\u3001PCR\u64f4\u589e\u7684\u512a\u9ede<\/p>\n\n\n\n

1.\u9ad8\u7279\u7570\u6027\uff1a\u900f\u904e\u8a2d\u8a08\u7279\u7570\u6027\u5f15\u5b50\uff0cPCR\u64f4\u589e\u80fd\u5920\u6e96\u78ba\u5730\u8907\u88fd\u76ee\u6a19DNA\u5e8f\u5217\uff0c\u907f\u514d\u975e\u7279\u7570\u6027\u64f4\u589e\u3002<\/p>\n\n\n\n

2.\u9ad8\u9748\u654f\u5ea6\uff1aPCR\u64f4\u589e\u80fd\u5920\u5f9e\u6975\u5c11\u91cf\u7684DNA\u6a23\u672c\u64f4\u589e\u51fa\u8db3\u5920\u7684\u76ee\u6a19\u7247\u6bb5\uff0c\u9069\u7528\u65bc\u5fae\u91cf\u6a23\u672c\u7684\u5206\u6790\u3002<\/p>\n\n\n\n

3.\u5feb\u901f\u6027\uff1a\u6574\u500bPCR\u64f4\u589e\u904e\u7a0b\u901a\u5e38\u5728\u5e7e\u500b\u5c0f\u6642\u5167\u5373\u53ef\u5b8c\u6210\uff0c\u5927\u5927\u63d0\u9ad8\u4e86\u5be6\u9a57\u6548\u7387\u3002<\/p>\n\n\n\n

\u5ee3\u6cdb\u61c9\u7528\uff1aPCR\u64f4\u589e\u6280\u8853\u5728\u57fa\u56e0\u514b\u9686\u3001\u7a81\u8b8a\u6aa2\u6e2c\u3001\u75c5\u539f\u9ad4\u6aa2\u6e2c\u3001\u89aa\u5b50\u9451\u5b9a\u7b49\u9818\u57df\u6709\u5ee3\u6cdb\u61c9\u7528\u3002<\/p>\n\n\n\n

\u4e94\u3001PCR\u64f4\u589e\u7684\u7f3a\u9ede<\/p>\n\n\n\n

1.\u6613\u53d7\u6c61\u67d3\u5f71\u97ff\uff1a\u7531\u65bcPCR\u64f4\u589e\u7684\u9ad8\u9748\u654f\u5ea6\uff0c\u5916\u6e90\u6027DNA\u6c61\u67d3\u53ef\u80fd\u5c0e\u81f4\u507d\u967d\u6027\u7d50\u679c\u3002\u56e0\u6b64\uff0c\u5be6\u9a57\u5ba4\u74b0\u5883\u548c\u64cd\u4f5c\u4eba\u54e1\u9700\u56b4\u683c\u9075\u5b88\u7121\u83cc\u64cd\u4f5c\u898f\u7a0b\u3002<\/p>\n\n\n\n

2.\u5f15\u5b50\u8a2d\u8a08\u8981\u6c42\u9ad8\uff1a\u5f15\u5b50\u7684\u8a2d\u8a08\u5c0dPCR\u64f4\u589e\u7684\u7279\u7570\u6027\u548c\u6548\u7387\u81f3\u95dc\u91cd\u8981\uff0c\u4e0d\u7576\u7684\u5f15\u5b50\u8a2d\u8a08\u53ef\u80fd\u5c0e\u81f4\u975e\u7279\u7570\u6027\u64f4\u589e\u6216\u64f4\u589e\u6548\u7387\u4f4e\u3002<\/p>\n\n\n\n

3.\u64f4\u589e\u7247\u6bb5\u9577\u5ea6\u6709\u9650\uff1a\u50b3\u7d71PCR\u64f4\u589e\u9069\u7528\u65bc1-3kb\u7684DNA\u7247\u6bb5\uff0c\u8f03\u9577\u7247\u6bb5\u7684\u64f4\u589e\u6548\u7387\u8f03\u4f4e\uff0c\u4e14\u9700\u8981\u7279\u6b8a\u7684\u64f4\u589e\u7cfb\u7d71\u3002<\/p>\n\n\n\n

PCR\u64f4\u589e\u4f5c\u70ba\u6838\u5fc3\u6280\u8853\uff0c\u56e0\u5176\u9ad8\u7279\u7570\u6027\u3001\u9ad8\u9748\u654f\u5ea6\u548c\u5feb\u901f\u6027\u800c\u88ab\u5ee3\u6cdb\u61c9\u7528\u65bc\u5404\u500b\u7814\u7a76\u9818\u57df\u3002\u96d6\u7136\u5b58\u5728\u6613\u53d7\u6c61\u67d3\u5f71\u97ff\u3001\u5f15\u5b50\u8a2d\u8a08\u8981\u6c42\u9ad8\u7b49\u7f3a\u9ede\uff0c\u4f46\u900f\u904e\u56b4\u683c\u7684\u5be6\u9a57\u64cd\u4f5c\u548c\u512a\u5316\u53cd\u61c9\u689d\u4ef6\uff0c\u9019\u4e9b\u554f\u984c\u53ef\u4ee5\u6709\u6548\u63a7\u5236\u3002\u672a\u4f86\uff0c\u96a8\u8457\u79d1\u6280\u7684\u4e0d\u65b7\u767c\u5c55\uff0cPCR\u64f4\u589e\u5fc5\u5c07\u5728\u66f4\u591a\u9818\u57df\u767c\u63ee\u66f4\u91cd\u8981\u7684\u4f5c\u7528\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u805a\u5408\u9176\u934a\u5f0f\u53cd\u61c9\uff08PCR\uff09\u64f4\u589e\u662f\u4e00\u7a2e\u91cd\u8981\u7684\u5206\u5b50\u751f\u7269\u5b78\u6280\u8853\uff0c\u88ab\u5ee3\u6cdb\u61c9\u7528\u65bc\u57fa\u56e0\u7814\u7a76\u3001\u91ab\u5b78\u8a3a\u65b7\u4ee5\u53ca\u6cd5\u91ab\u5b78\u9451\u5b9a\u3002\u672c\u6587\u5c07\u8a73 […]<\/p>\n","protected":false},"author":5,"featured_media":3732,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[],"tags":[],"class_list":["post-3730","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"yoast_head":"\nPCR\u6269\u589e - Opentrons\u4e2d\u56fd\u5b98\u7f51<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/opentrons.com.cn\/zh-TW\/news\/pcr-kuozeng\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR\u6269\u589e - 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