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1.qPCR\u5100\u5668\uff1a\u9019\u662f\u6838\u5fc3\u8a2d\u5099\uff0c\u914d\u5099\u87a2\u5149\u6aa2\u6e2c\u7cfb\u7d71\uff0c\u7528\u65bc\u76e3\u63a7PCR\u53cd\u61c9\u904e\u7a0b\u4e2d\u7684\u87a2\u5149\u8a0a\u865f\u8b8a\u5316\u3002<\/p>\n\n\n\n
2.\u71b1\u5faa\u74b0\u5100\uff1a\u63a7\u5236\u53cd\u61c9\u9ad4\u7cfb\u7684\u6eab\u5ea6\u8b8a\u5316\uff0c\u5be6\u73feDNA\u7684\u8b8a\u6027\u3001\u9000\u706b\u8207\u5ef6\u4f38\u3002<\/p>\n\n\n\n
3.\u5149\u5b78\u6aa2\u6e2c\u7cfb\u7d71\uff1a\u7528\u65bc\u6aa2\u6e2c\u53cd\u61c9\u7ba1\u5167\u7684\u87a2\u5149\u8a0a\u865f\uff0c\u4e26\u5c07\u5176\u8f49\u63db\u70ba\u96fb\u8a0a\u865f\u9032\u884c\u5206\u6790\u3002<\/p>\n\n\n\n
4.\u8edf\u9ad4\uff1a\u7528\u65bc\u8cc7\u6599\u7684\u63a1\u96c6\u3001\u8655\u7406\u8207\u5206\u6790\uff0c\u7522\u751f\u64f4\u589e\u66f2\u7dda\u53ca\u6a19\u6e96\u66f2\u7dda\u7b49\u7d50\u679c\u3002<\/p>\n\n\n\n
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1.\u6a23\u672c\u6e96\u5099\uff1a\u8403\u53d6\u6a23\u672c\u4e2d\u7684DNA\u6216RNA\uff0c\u4e26\u900f\u904e\u9006\u8f49\u9304\u9176\u5c07RNA\u8f49\u5316\u70bacDNA\u3002<\/p>\n\n\n\n
2.\u53cd\u61c9\u9ad4\u7cfb\u914d\u88fd\uff1a\u5305\u62ec\u5f15\u5b50\u3001\u63a2\u91dd\u6216\u67d3\u6599\u3001dNTP\u3001Taq\u9176\u3001\u7de9\u885d\u6db2\u53ca\u6a21\u677f\u6838\u9178\u7b49\u6210\u5206\u7684\u6df7\u5408\u3002<\/p>\n\n\n\n
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