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OT-2

Putting laboratory automation within reach

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Easy lab automation

The OT-2 benchtop pipetting robot is flexible and compatible and is suitable for a variety of common laboratory automation processes.

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Evaluate

  • OT-2 is really easy to use and we feel much more relaxed.

    – Maripavan, Boston university

  • These days we transfer 800 samples using the Opentrons automated pipetting platform. I could never process so many samples manually.

    – KA jaw Ask, gen cove

  • In the past, I had to spend a whole day on an ELISA experiment. Now the automatic pipetting robot frees my hands.

    – Annabel shang, mill IPO hot sigma

  • We are discussing with finance and plan to purchase two more OT-2s. We like this platform very much.

    – Adam Ericson, Emory university

The most time-consuming processes in automated laboratories

  • NGS library preparation
  • PCR settings
  • protein purification
  • Nucleic acid extraction and purification
  • Routine reagent packaging

NGS library preparation

NGS library preparation is one of the most common automated applications for the OT-2 automated pipetting platform. It automates not only NGS library preparation, but also other steps of the pre-sequencing workflow, including reagent dispensing, bead cleanup, sample concentration normalization, and pooling. You can use your own protocol, or choose from our online protocol library a protocol that has been validated by the Opentrons scientific team in similar applications or a protocol provided by a major reagent brand currently on the market.

Compact model, powerful function

Only half the size of a standard laboratory bench

  • Metric (width, depth, height) 63 mammoth 57 mammoth 66 smart
  • Imperial (width, depth, height) 25 in small 22.5 in small 26 in

Performance comparable to similar devices costing 10 times more

  • Accuracy 15% at 1 μL
  • Accuracy 5% at 1 μL

View complete specifications

Start your laboratory automation

We provide you with the professional technical support you need for laboratory automation

  • Online demo

    Provide OT-2 online demonstration to answer your application questions

    預(yù)約演示

  • Order OT-2

    Select OT-2, pipettes, related modules and labware

    預(yù)約演示

  • OT-2 Receiving and unpacking

    We provide unpacking instructions to help you complete the unpacking process

    預(yù)約演示

  • Customization and customer service support

    On-site installation, development of customized protocols, etc.

    預(yù)約演示

Use what you use to increase what you need

Hardware and pipettes

  • The OT-2 features 11 deck slots, a waste slot, and a pipetting arm that can be equipped with two interchangeable single-channel and 8-channel pipettes.
  • You can add magnetic bead purification module, temperature control module, thermal cycle module, thermal shaker and HEPA module according to your needs

Works with existing labware and reagents

  • No restrictions on reagents and consumables
  • Compatible with any ANSI/SLAS compliant lab consumables

How to find the protocol creation method that works best for you

A protocol is the code used to run the instrument through your experiment. We've established different ways to create protocols, so whether you're new to automation or an experienced Python programmer, there's something for you.

Pipetting arm that can be equipped with 2 pipettes

Precise liquid transfers from 1–1000 μL are possible with our interchangeable 1- and 8-channel pipettes

  • 1 channel
    • P20
    • P300
    • P1000
  • 8 channels
    • P20
    • P300

Support and service

Provide you with the support services you need at any time

  • Online customer service

    Online customer service provides consulting services through chat

  • knowledge hub

    Visit the Knowledge Center to browse more in-depth support articles and videos

  • Customized Agreement

    A dedicated team of application scientists can develop custom protocols for you

  • Warranty service

    Support live chat and provide technical support

Technical parameters

  • size

    63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (width, depth, height)

    Occupies half of a standard laboratory bench

  • weight

    48kg or 105.8lb

  • Pipette configuration

    Single and 8-channel pipetting

    2 pipette mounts for configuring 1 or 2 single or 8-channel pipettes. Pipettes are easily interchangeable.

  • Pipette capacity and specifications
  • deck

    11 deck slots allow for countless configurations

    Deck slots are compatible with standard SBS dimensions.

    The deck also includes a removable trash can.

  • Connectivity

    WiFi 2.4 GHz IEEE 802.11, USB 2.0

    learn more
  • video

    Integrated camera (included for future or custom functions)

  • Audio

    Integrated audio system (included for future or custom features)

  • power supply

    Robot power input: 36 VDC, 6.1 A

    Power adapter input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC

    For additional power requirement information, check out this support article

  • frame

    Rigid steel and CNC aluminum design

  • Operating temperature and relative humidity

    Recommended 20-24 °C

    Recommended 40-60% RH

    learn more
  • 96-well plate filling time
    Pipette Distribution
    One Tip    		 Transfer
    Same Tip    		 Transfer
    New Tip
    P20 8 channels 0:22 1:35 4:19
    P300 8 channels 0:26 1:38 4:24
    P20 single channel 3:12 13:10 36:01
    P300 single channel 4:01 13:39 41:31
    P1000 5:54 15:21 44:50
  • Single channel accuracy and precision
    Pipette Measuring range Random
    error    		 System
    Error
    %D μl %CV μl
    P20 1 10 20 ±15%
    ±2%
    ±1.5%    		 0.15μl
    0.2μl
    0.3μl    		 ±5%
    ±1%
    ±0.8%    		 0.05μl
    0.1μl
    0.16μl
    P300 20 150 300 ±4%
    ±1%
    ±0.6%    		 0.8μl
    1.5μl
    1.8μl    		 ±2.5%
    ±0.4%
    ±0.3%    		 0.05μl
    0.6μl
    0.9μ
    P1000 100 500 1000 ±2%
    ±1%
    ±0.7%    		 2.0μl
    5.0μl
    7.0μl    		 ±1%
    ±0.2%
    ±0.15%    		 1μl
    1μl
    1.5μl
  • 8-channel accuracy and precision
    Pipette Measuring range Random
    error    		 System
    Error
    %D μl %CV μl
    P20 1 10 20 ±20%
    ±3%
    ±2.2%    		 0.2μl
    0.3μl
    0.44μl    		 ±10%
    ±2%
    ±1.5%    		 0.1μl
    0.2μl
    0.3μl
    P300 20 150 300 ±10%
    ±2.5%
    ±1.5%    		 2.0μl
    3.75μl
    4.5μl    		 ±4%
    ±0.8%
    ±0.5%    		 0.8μl
    1.2μl
    1.5μl
  • Sterility

    Full polycarbonate shell designed to limit exposure. OT-2 is not a sterile environment

    Add Opentrons HEPA module or add your own UV lamp

  • Viscosity

    For best practices in automated viscous liquid handling, check out our application notes

    Viscous liquid handling automation with Opentrons OT-2

  • API compatibility

    Interface with any product that has an API

    Opentrons software is open source and users can adapt the software as needed to interface with other products. While all integrations are possible, they all require custom code work, i.e. most customers will need to write custom libraries and install packages that don't come with the robot.

    learn more
  • operating system

    Windows 10, macOS 10.10 or higher, Ubuntu 12.04 or higher.

    open TR ONS app
  • Tip compatibility

    USE Opentrons tips and Opentrons filter tips

    Opentrons guarantees the performance of Opentrons tips designed specifically for the OT-2. The OT-2 is designed to accommodate all universal hole tip types, but tip quality and size can vary widely, so Opentrons cannot guarantee third-party tip performance.

  • Labware Compatibility

    Use common labware already in our labware library, or add your own. If you have labware that is suitable for the OT-2 but is not in the Labware Library, you can add it using our Labware Creator or our team.

    learn more OT-2 Manual
  • Warranty

    One-year warranty against manufacturer defects

    Stress tested to last at least 3 years of full-time use


  • Certification

    Certified (CE, FCC, NRTL, CB, ISO 9001)

    Not certified/validated (IVD, GMP)

  • Command example
    Tip handling

    Pick up tips

    Put down the suction head

    Return tips

    New suction tip

    More…

    Liquid control

    suck out

    distribute

    Extraction speed

    Distribution speed

    blow empty

    Touch the tip

    mix

    air gap

    Utility tools

    Move to

    Delay

    user specified pause

    Return to zero

    Comment

    Transmission

    Basic transfer

    Volume > Pipette Volume

    distribution

    merge

    Variable settings

    speed

    volume

    count

    Other

    throw away

  • Packing list

    OT-2 robot

    calibration module

    Removable module

    trash can

    Quick start guide

    Power and zone IEC cables

    Ethernet cable

    Ethernet to USB Adapter

    tool kit

Easy lab automation

The OT-2 benchtop pipetting robot is flexible and compatible and is suitable for a variety of common laboratory automation processes.

Watch Video

Resource

FAQ

Frequently Asked Questions about Opentrons Flex and OT-2 Products，Please click here.

  • What kind of labware is the OT-2 compatible with?

    Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS-compliant orifice plate, as well as a wide variety of EP tubes and screw-cap centrifuge tubes, including centrifuge tubes with volumes up to 50 mL. You can view all supported labware in our labware library.

  • Do I need to know programming knowledge to use OT-2?

    No programming knowledge is required to use the Opentrons platform. You can use Opentrons' concise and easy to understand protocol design tools to create protocols. In addition, our application engineering technology team can also Create a custom agreement for you.

  • What does the OT-2 protocol look like?

    You can find our community's OT-2 protocol set in our protocol library. You can search to find protocols that match your laboratory workflow! If you cannot find a protocol that matches your laboratory process, we offer several different methods to create your protocol. In addition, you can also contact us to have our engineering team develop custom protocols for you.

  • Can the OT-2 be integrated with third-party lab equipment?

    Due to the open-source advantage of OT-2, many users have successfully integrated OT-2 with Third-party laboratory equipment.

  • What certifications does the OT-2 have?

    OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB. Please note that this OT-2 is not IVD or GMP certified/validated.

  • Can OT-2 be used in a biological safety cabinet?

    Labs within the Opentrons community have installed OT-2 automated pipetting platforms in biosafety cabinets, including Labconco’s Logic Vue Class II closure device and Baker’s AeroPROTECT 360 closure device. We recommend that you evaluate the size of the OT-2 by comparing it with the size of the biological safety cabinet you choose.

Learn More

Welcome to make an appointment for an online demonstration to discuss your experimental needs with a team of professional application scientists.

Book a demo
Contact Us

The experienced service team and strong production support team provide customers with worry-free order services.

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